Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           19     869
NP_055903                                                              19     869
XP_006723678                                                           17    2528
NP_003236                                                              17    2528
NP_001269399                                                           17    3301
NP_055434                                                              17    3301
NP_002079                                                              17    4312
XP_006723233                                                           17    4312
XP_006723232                                                           17    4312
XP_005258879                                                           17    4312
XP_005258878                                                           17    4312
NP_056138                                                              16    7355
NP_004733                                                              16    7355
NP_001028229                                                           16    7355
NP_056335                                                              16    7355
NP_061889                                                              16    7355
NP_998823                                                              16    7355
NP_998822                                                              16    7355
NP_055532                                                              16    7355
NP_015567                                                              16    7355
XP_006723575                                                           16    7355
XP_005259502                                                           16    7355
XP_006721802                                                           16    7355
XP_005253417                                                           16    7355
XP_005265631                                                           16    7355
XP_006713477                                                           16    7355
XP_006713476                                                           16    7355
XP_005265628                                                           16    7355
XP_005265627                                                           16    7355
XP_006713475                                                           16    7355
XP_006713474                                                           16    7355
XP_005265625                                                           16    7355
XP_006713473                                                           16    7355
XP_005265623                                                           16    7355
XP_005265622                                                           16    7355
XP_005265621                                                           16    7355
XP_005265620                                                           16    7355
XP_006713472                                                           16    7355
XP_006713471                                                           16    7355
XP_006713470                                                           16    7355
XP_005245090                                                           16    7355
XP_005245089                                                           16    7355
XP_006711303                                                           16    7355
XP_005245088                                                           16    7355
NP_002250                                                              16    7355
NP_056018                                                              16    7355
NP_005949                                                              16    7355
NP_001813                                                              15    9605
NP_001020372                                                           15    9605
NP_001193531                                                           15    9605
NP_004058                                                              15    9605
NP_001035025                                                           15    9605
NP_079146                                                              15    9605
NP_001034                                                              15    9605
NP_001165972                                                           15    9605
NP_001165973                                                           15    9605
NP_001127891                                                           15    9605
NP_001193987                                                           15    9605
NP_059115                                                              15    9605
NP_001631                                                              15    9605
NP_060423                                                              15    9605
NP_112207                                                              15    9605
NP_001165975                                                           15    9605
NP_852100                                                              15    9605
XP_006721326                                                           15    9605
XP_006720771                                                           15    9605
XP_005249659                                                           15    9605
XP_005249656                                                           15    9605
XP_005265155                                                           15    9605
XP_005265154                                                           15    9605
XP_005271301                                                           15    9605
XP_005271298                                                           15    9605
XP_006710766                                                           15    9605
NP_001127892                                                           15    9605
NP_003494                                                              15    9605
NP_001012780                                                           15    9605
NP_001012777                                                           15    9605
>NP_001155973
          Length = 1833

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           MENAGV FV
Sbjct: 350 MENAGVNFV 358
>NP_055903
          Length = 1723

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           MENAGV FV
Sbjct: 350 MENAGVNFV 358
>XP_006723678
          Length = 718

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ENAGVIFV 9
           E+AG+IFV
Sbjct: 183 EDAGIIFV 190
>NP_003236
          Length = 693

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ENAGVIFV 9
           E+AG+IFV
Sbjct: 158 EDAGIIFV 165
>NP_001269399
          Length = 956

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IFV
Sbjct: 802 MENIGGIFV 810
>NP_055434
          Length = 956

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IFV
Sbjct: 802 MENIGGIFV 810
>NP_002079
          Length = 980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IF+
Sbjct: 801 MENIGGIFI 809
>XP_006723233
          Length = 912

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IF+
Sbjct: 733 MENIGGIFI 741
>XP_006723232
          Length = 981

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IF+
Sbjct: 801 MENIGGIFI 809
>XP_005258879
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IF+
Sbjct: 763 MENIGGIFI 771
>XP_005258878
          Length = 980

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   MENAGVIFV 9
           MEN G IF+
Sbjct: 801 MENIGGIFI 809
>NP_056138
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           ++N GVIF
Sbjct: 171 LDNRGVIF 178
>NP_004733
          Length = 1256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>NP_001028229
          Length = 1462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>NP_056335
          Length = 1287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>NP_061889
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           +ENAG+I
Sbjct: 211 VENAGII 217
>NP_998823
          Length = 233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MENAGVIF 8
         M+N GVI+
Sbjct: 1 MDNQGVIY 8
>NP_998822
          Length = 215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MENAGVIF 8
         M+N GVI+
Sbjct: 1 MDNQGVIY 8
>NP_055532
          Length = 1300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           + NAGV+F
Sbjct: 552 LANAGVLF 559
>NP_015567
          Length = 215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MENAGVIF 8
         M+N GVI+
Sbjct: 1 MDNQGVIY 8
>XP_006723575
          Length = 1302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           + NAGV+F
Sbjct: 552 LANAGVLF 559
>XP_005259502
          Length = 1313

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           + NAGV+F
Sbjct: 565 LANAGVLF 572
>XP_006721802
          Length = 427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           +ENAG+I
Sbjct: 113 VENAGII 119
>XP_005253417
          Length = 228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MENAGVIF 8
         M+N GVI+
Sbjct: 1 MDNQGVIY 8
>XP_005265631
          Length = 1288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713477
          Length = 1290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713476
          Length = 1375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 110 MQNAGIV 116
>XP_005265628
          Length = 1395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005265627
          Length = 1425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713475
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713474
          Length = 1465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005265625
          Length = 1478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713473
          Length = 1479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005265623
          Length = 1479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005265622
          Length = 1481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005265621
          Length = 1491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005265620
          Length = 1492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713472
          Length = 1492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713471
          Length = 1492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_006713470
          Length = 1493

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   MENAGVI 7
           M+NAG++
Sbjct: 228 MQNAGIV 234
>XP_005245090
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           +ENAG+ +
Sbjct: 271 LENAGIFY 278
>XP_005245089
          Length = 934

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           +ENAG+ +
Sbjct: 245 LENAGIFY 252
>XP_006711303
          Length = 960

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           +ENAG+ +
Sbjct: 271 LENAGIFY 278
>XP_005245088
          Length = 1435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           +ENAG+ +
Sbjct: 245 LENAGIFY 252
>NP_002250
          Length = 233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 MENAGVIF 8
         M+N GVI+
Sbjct: 1 MDNQGVIY 8
>NP_056018
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   MENAGVIF 8
           +ENAG+ +
Sbjct: 271 LENAGIFY 278
>NP_005949
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  MENAGVIFV 9
          + NAG IFV
Sbjct: 58 LRNAGNIFV 66
>NP_001813
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 416 ENLGIVF 422
>NP_001020372
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 390 ENLGIVF 396
>NP_001193531
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 291 ENLGIVF 297
>NP_004058
          Length = 468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 425 ENLGIVF 431
>NP_001035025
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 289 ENLGIVF 295
>NP_079146
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    NAGVIF 8
            NAGV+F
Sbjct: 1313 NAGVLF 1318
>NP_001034
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           E AG++F+
Sbjct: 382 EGAGLVFI 389
>NP_001165972
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           E AG++F+
Sbjct: 382 EGAGLVFI 389
>NP_001165973
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           E AG++F+
Sbjct: 277 EGAGLVFI 284
>NP_001127891
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           M +AGVIF+
Sbjct: 397 MWSAGVIFL 405
>NP_001193987
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  NAGVIFV 9
          N G+IF+
Sbjct: 26 NTGIIFI 32
>NP_059115
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NAGVIFV 9
           +AG+IFV
Sbjct: 250 DAGIIFV 256
>NP_001631
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NAGVIFV 9
           +AGV+FV
Sbjct: 255 DAGVVFV 261
>NP_060423
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           ENAGV+ V
Sbjct: 110 ENAGVLEV 117
>NP_112207
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   ENAGVIF 8
           +N+G+IF
Sbjct: 131 QNSGIIF 137
>NP_001165975
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           E AG++F+
Sbjct: 382 EGAGLVFI 389
>NP_852100
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   ENAGVIF 8
           +N+G+IF
Sbjct: 204 QNSGIIF 210
>XP_006721326
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           E AG++F+
Sbjct: 382 EGAGLVFI 389
>XP_006720771
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  NAGVIFV 9
          N G+IF+
Sbjct: 26 NTGIIFI 32
>XP_005249659
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 218 ENLGIVF 224
>XP_005249656
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   ENAGVIF 8
           EN G++F
Sbjct: 244 ENLGIVF 250
>XP_005265155
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NAGVIFV 9
           +AGV+FV
Sbjct: 154 DAGVVFV 160
>XP_005265154
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NAGVIFV 9
           +AGV+FV
Sbjct: 209 DAGVVFV 215
>XP_005271301
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           M +AGVIF+
Sbjct: 365 MWSAGVIFL 373
>XP_005271298
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           M +AGVIF+
Sbjct: 397 MWSAGVIFL 405
>XP_006710766
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ENAGVIFV 9
           ENAGV+ V
Sbjct: 110 ENAGVLEV 117
>NP_001127892
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           M +AGVIF+
Sbjct: 397 MWSAGVIFL 405
>NP_003494
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   MENAGVIFV 9
           M +AGVIF+
Sbjct: 397 MWSAGVIFL 405
>NP_001012780
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AGVIFV 9
           AGVIFV
Sbjct: 108 AGVIFV 113
>NP_001012777
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AGVIFV 9
           AGVIFV
Sbjct: 108 AGVIFV 113
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.145    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 473,640
Number of extensions: 865
Number of successful extensions: 77
Number of sequences better than 10000.0: 77
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 77
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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