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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001276866                                                           17    2528
NP_004439                                                              17    2528
NP_001276865                                                           17    2528
NP_001005862                                                           17    2528
XP_005257197                                                           17    2528
XP_006721828                                                           17    2528
NP_001013660                                                           15    9605
NP_689619                                                              15    9605
NP_001139809                                                           15    9605
NP_001003760                                                           15    9605
XP_005249164                                                           15    9605
XP_005268430                                                           15    9605
XP_006713469                                                           15    9605

>NP_001276866
          Length = 1055

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTSIISAAV 9
           LTSIISA V
Sbjct: 651 LTSIISAVV 659



>NP_004439
          Length = 1255

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTSIISAAV 9
           LTSIISA V
Sbjct: 651 LTSIISAVV 659



>NP_001276865
          Length = 1240

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTSIISAAV 9
           LTSIISA V
Sbjct: 636 LTSIISAVV 644



>NP_001005862
          Length = 1225

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTSIISAAV 9
           LTSIISA V
Sbjct: 621 LTSIISAVV 629



>XP_005257197
          Length = 1225

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTSIISAAV 9
           LTSIISA V
Sbjct: 621 LTSIISAVV 629



>XP_006721828
          Length = 1336

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LTSIISAAV 9
           LTSIISA V
Sbjct: 732 LTSIISAVV 740



>NP_001013660
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TSIISAAV 9
           TS+ISA V
Sbjct: 319 TSVISAGV 326



>NP_689619
          Length = 768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LTSIISA 7
           LTSI+SA
Sbjct: 530 LTSIVSA 536



>NP_001139809
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LTSIISA 7
           LTSI+SA
Sbjct: 530 LTSIVSA 536



>NP_001003760
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SIISAAV 9
           SIISAAV
Sbjct: 142 SIISAAV 148



>XP_005249164
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SIISAAV 9
           SIISAAV
Sbjct: 142 SIISAAV 148



>XP_005268430
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LTSIISA 7
           LTSI+SA
Sbjct: 497 LTSIVSA 503



>XP_006713469
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  TSIISAA 8
          TSI+SAA
Sbjct: 33 TSIVSAA 39



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.123    0.264 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 209,412
Number of extensions: 100
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)