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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443200                                                              18    1935
NP_001160070                                                           18    1935
XP_006718824                                                           18    1935
NP_004594                                                              17    4312
NP_001159375                                                           17    4312
XP_005250609                                                           17    4312
NP_996888                                                              16    5631
NP_009053                                                              16    5631
NP_001263302                                                           16    5631
NP_001274745                                                           15    9605
NP_001001998                                                           15    9605
NP_443106                                                              15    9605
XP_005262351                                                           15    9605
XP_006719367                                                           15    9605
XP_005245729                                                           15    9605
XP_005245726                                                           15    9605
XP_006711745                                                           15    9605
XP_005263532                                                           15    9605
NP_002676                                                              15    9605

>NP_443200
          Length = 366

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  LSAYSATQS 9
          LSA+SATQ+
Sbjct: 15 LSAFSATQA 23



>NP_001160070
          Length = 366

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  LSAYSATQS 9
          LSA+SATQ+
Sbjct: 15 LSAFSATQA 23



>XP_006718824
          Length = 366

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  LSAYSATQS 9
          LSA+SATQ+
Sbjct: 15 LSAFSATQA 23



>NP_004594
          Length = 288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSAYSATQS 9
           +S Y+ATQS
Sbjct: 131 MSEYNATQS 139



>NP_001159375
          Length = 251

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSAYSATQS 9
           +S Y+ATQS
Sbjct: 131 MSEYNATQS 139



>XP_005250609
          Length = 280

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSAYSATQS 9
           +S Y+ATQS
Sbjct: 131 MSEYNATQS 139



>NP_996888
          Length = 251

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LSAYSATQS 9
          +S Y ATQS
Sbjct: 22 ISGYPATQS 30



>NP_009053
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LSAYSATQS 9
          +S Y ATQS
Sbjct: 81 ISGYPATQS 89



>NP_001263302
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LSAYSATQS 9
          +S Y ATQS
Sbjct: 81 ISGYPATQS 89



>NP_001274745
          Length = 1078

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAYSATQS 9
            S YS TQS
Sbjct: 995  SVYSKTQS 1002



>NP_001001998
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  LSAYSATQS 9
          LSA SAT+S
Sbjct: 12 LSATSATKS 20



>NP_443106
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSAYSATQS 9
           ++ Y+ATQS
Sbjct: 130 MTEYNATQS 138



>XP_005262351
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LSAYSAT 7
           L AYSAT
Sbjct: 389 LQAYSAT 395



>XP_006719367
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSAYSATQS 9
           LSA+S T+S
Sbjct: 857 LSAHSETES 865



>XP_005245729
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SAYSATQS 9
           S YS TQS
Sbjct: 984 SVYSKTQS 991



>XP_005245726
          Length = 1095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAYSATQS 9
            S YS TQS
Sbjct: 995  SVYSKTQS 1002



>XP_006711745
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SAYSATQS 9
            S YS TQS
Sbjct: 995  SVYSKTQS 1002



>XP_005263532
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  LSAYSATQS 9
          LSA SAT+S
Sbjct: 36 LSATSATKS 44



>NP_002676
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  LSAYSATQS 9
          LSA SAT+S
Sbjct: 12 LSATSATKS 20



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.291   0.0950    0.214 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 341,234
Number of extensions: 463
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)