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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              17    2528
NP_001273501                                                           16    5631
NP_055586                                                              16    5631
NP_001193962                                                           16    5631
NP_001193963                                                           16    5631
NP_037514                                                              16    7355
NP_001276332                                                           15    9605
NP_001276334                                                           15    9605
NP_071757                                                              15    9605
NP_075055                                                              15    9605
XP_005260077                                                           15    9605
XP_006718007                                                           15    9605

>NP_055365
          Length = 1032

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LRSRIRAIQ 9
           L SRIRAIQ
Sbjct: 904 LGSRIRAIQ 912



>NP_001273501
          Length = 1001

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRSRIRAIQ 9
           LRSRIR ++
Sbjct: 703 LRSRIRELE 711



>NP_055586
          Length = 1001

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRSRIRAIQ 9
           LRSRIR ++
Sbjct: 703 LRSRIRELE 711



>NP_001193962
          Length = 1000

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRSRIRAIQ 9
           LRSRIR ++
Sbjct: 703 LRSRIRELE 711



>NP_001193963
          Length = 901

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRSRIRAIQ 9
           LRSRIR ++
Sbjct: 603 LRSRIRELE 611



>NP_037514
          Length = 750

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LRSRIRAI 8
           LRSRIR I
Sbjct: 470 LRSRIRFI 477



>NP_001276332
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RSRIRAIQ 9
          R+R RAIQ
Sbjct: 63 RTRARAIQ 70



>NP_001276334
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RSRIRAIQ 9
          R+R RAIQ
Sbjct: 63 RTRARAIQ 70



>NP_071757
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RSRIRAIQ 9
          R+R RAIQ
Sbjct: 63 RTRARAIQ 70



>NP_075055
          Length = 1011

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRSRIRA 7
           LR+RIRA
Sbjct: 415 LRARIRA 421



>XP_005260077
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRSRIRA 7
           LR+RIRA
Sbjct: 409 LRARIRA 415



>XP_006718007
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  RSRIRAIQ 9
          R+R RAIQ
Sbjct: 84 RTRARAIQ 91



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.146    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 298,009
Number of extensions: 353
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)