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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     299
NP_000706                                                              17    3301
XP_005273308                                                           17    3301
XP_005273309                                                           17    3301
NP_653267                                                              17    4312
NP_065845                                                              17    4312
XP_006710845                                                           17    4312
NP_061029                                                              16    7355
NP_057043                                                              16    7355
NP_075462                                                              16    7355
NP_001166281                                                           16    7355
NP_001137484                                                           16    7355
XP_006723289                                                           16    7355
XP_005257635                                                           16    7355
XP_005266501                                                           16    7355
XP_005266500                                                           16    7355
NP_001175                                                              15    9605
NP_001265473                                                           15    9605
NP_000057                                                              15    9605
NP_055736                                                              15    9605
NP_068817                                                              15    9605
NP_001265472                                                           15    9605
NP_001008701                                                           15    9605
NP_001239160                                                           15    9605
XP_005259876                                                           15    9605
XP_006722752                                                           15    9605
XP_005259875                                                           15    9605
XP_006722751                                                           15    9605
XP_005271911                                                           15    9605
XP_006714586                                                           15    9605
XP_005271223                                                           15    9605
XP_006710936                                                           15    9605

>NP_004351
          Length = 882

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  LNTRFKVGT 9
          L+TRFKVGT
Sbjct: 71 LDTRFKVGT 79



>NP_000706
          Length = 597

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  TRFKVGT 9
          TRFK GT
Sbjct: 69 TRFKTGT 75



>XP_005273308
          Length = 597

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  TRFKVGT 9
          TRFK GT
Sbjct: 69 TRFKTGT 75



>XP_005273309
          Length = 597

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  TRFKVGT 9
          TRFK GT
Sbjct: 69 TRFKTGT 75



>NP_653267
          Length = 4753

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LNTRFKV 7
            LN+RFKV
Sbjct: 1284 LNSRFKV 1290



>NP_065845
          Length = 1537

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTRFKVGT 9
           NTR KVG+
Sbjct: 701 NTRVKVGS 708



>XP_006710845
          Length = 1492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NTRFKVGT 9
           NTR KVG+
Sbjct: 656 NTRVKVGS 663



>NP_061029
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LNTRFKVG 8
           LNTRF  G
Sbjct: 327 LNTRFSHG 334



>NP_057043
          Length = 296

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNTRFKVGT 9
           ++TR+KV T
Sbjct: 202 VSTRYKVST 210



>NP_075462
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LNTRFKVGT 9
           L T+F VGT
Sbjct: 329 LPTKFMVGT 337



>NP_001166281
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LNTRFKVGT 9
           L T+F VGT
Sbjct: 329 LPTKFMVGT 337



>NP_001137484
          Length = 258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNTRFKVGT 9
           ++TR+KV T
Sbjct: 164 VSTRYKVST 172



>XP_006723289
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   LNTRFKVGT 9
           + TRF  GT
Sbjct: 136 MGTRFSAGT 144



>XP_005257635
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LNTRFKVGT 9
           L T+F VGT
Sbjct: 329 LPTKFMVGT 337



>XP_005266501
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LNTRFKVG 8
           LNTRF  G
Sbjct: 327 LNTRFSHG 334



>XP_005266500
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LNTRFKVG 8
           LNTRF  G
Sbjct: 327 LNTRFSHG 334



>NP_001175
          Length = 2644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LNTRFK 6
            LNTR+K
Sbjct: 1458 LNTRYK 1463



>NP_001265473
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LNTRFK 6
           LNTRF+
Sbjct: 144 LNTRFR 149



>NP_000057
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LNTRFK 6
           LNTRF+
Sbjct: 206 LNTRFR 211



>NP_055736
          Length = 1469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NTRFKVGT 9
            NTR+  GT
Sbjct: 1133 NTRYYTGT 1140



>NP_068817
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NTRFKVG 8
           NTR K+G
Sbjct: 539 NTRTKIG 545



>NP_001265472
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LNTRFK 6
           LNTRF+
Sbjct: 154 LNTRFR 159



>NP_001008701
          Length = 1474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NTRFKVGT 9
            NTR+  GT
Sbjct: 1138 NTRYYTGT 1145



>NP_001239160
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NTRFKVG 8
           NTR K+G
Sbjct: 539 NTRTKIG 545



>XP_005259876
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NTRFKVGT 9
            NTR+  GT
Sbjct: 1138 NTRYYTGT 1145



>XP_006722752
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NTRFKVGT 9
            NTR+  GT
Sbjct: 1102 NTRYYTGT 1109



>XP_005259875
          Length = 1493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NTRFKVGT 9
            NTR+  GT
Sbjct: 1133 NTRYYTGT 1140



>XP_006722751
          Length = 1498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    NTRFKVGT 9
            NTR+  GT
Sbjct: 1138 NTRYYTGT 1145



>XP_005271911
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NTRFKVG 8
           NTR K+G
Sbjct: 539 NTRTKIG 545



>XP_006714586
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NTRFKVG 8
           NTR K+G
Sbjct: 481 NTRTKIG 487



>XP_005271223
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LNTRFK 6
           LNTRF+
Sbjct: 243 LNTRFR 248



>XP_006710936
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   LNTRFK 6
           LNTRF+
Sbjct: 245 LNTRFR 250



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.139    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 386,638
Number of extensions: 519
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)