Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              17    4312
XP_006718908                                                           17    4312
XP_005271621                                                           17    4312
XP_006718907                                                           17    4312
XP_006718906                                                           17    4312
XP_005271619                                                           17    4312
XP_005271618                                                           17    4312
NP_065390                                                              16    7355
NP_056505                                                              16    7355
NP_001230242                                                           16    7355
NP_001008392                                                           16    7355
XP_006712484                                                           16    7355
XP_006712483                                                           16    7355
XP_005246521                                                           16    7355
NP_008862                                                              15    9605
NP_002824                                                              15    9605
NP_775876                                                              15    9605
NP_443120                                                              15    9605
NP_001107875                                                           15    9605
XP_006716284                                                           15    9605
XP_006716282                                                           15    9605
>NP_000042
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LLVSLLLKE 9
            L VSLLLK+
Sbjct: 1444 LFVSLLLKD 1452
>XP_006718908
          Length = 1708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LLVSLLLKE 9
           L VSLLLK+
Sbjct: 96  LFVSLLLKD 104
>XP_005271621
          Length = 2708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LLVSLLLKE 9
            L VSLLLK+
Sbjct: 1096 LFVSLLLKD 1104
>XP_006718907
          Length = 3050

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LLVSLLLKE 9
            L VSLLLK+
Sbjct: 1438 LFVSLLLKD 1446
>XP_006718906
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LLVSLLLKE 9
            L VSLLLK+
Sbjct: 1444 LFVSLLLKD 1452
>XP_005271619
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LLVSLLLKE 9
            L VSLLLK+
Sbjct: 1444 LFVSLLLKD 1452
>XP_005271618
          Length = 3056

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LLVSLLLKE 9
            L VSLLLK+
Sbjct: 1444 LFVSLLLKD 1452
>NP_065390
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LVSLLLK 8
           LVSLLLK
Sbjct: 232 LVSLLLK 238
>NP_056505
          Length = 1315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LVSLLLKE 9
           LVSL+LK+
Sbjct: 208 LVSLILKD 215
>NP_001230242
          Length = 1294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LVSLLLKE 9
           LVSL+LK+
Sbjct: 208 LVSLILKD 215
>NP_001008392
          Length = 1079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VSLLLKE 9
           +SLLLKE
Sbjct: 928 ISLLLKE 934
>XP_006712484
          Length = 851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LVSLLLKE 9
           LVSL+LK+
Sbjct: 208 LVSLILKD 215
>XP_006712483
          Length = 1306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LVSLLLKE 9
           LVSL+LK+
Sbjct: 208 LVSLILKD 215
>XP_005246521
          Length = 1315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LVSLLLKE 9
           LVSL+LK+
Sbjct: 208 LVSLILKD 215
>NP_008862
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLVSLLLKE 9
           + VSLLLK+
Sbjct: 349 MTVSLLLKD 357
>NP_002824
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LVSLLLKE 9
           ++SLLLK+
Sbjct: 201 IISLLLKD 208
>NP_775876
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LLVSLLLKE 9
           LL+  LLKE
Sbjct: 213 LLIDTLLKE 221
>NP_443120
          Length = 947

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LVSLLLKE 9
          L+SLL+KE
Sbjct: 17 LLSLLVKE 24
>NP_001107875
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LLVSLLL 7
          LLVSLLL
Sbjct: 20 LLVSLLL 26
>XP_006716284
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LLVSLLL 7
          LLVSLLL
Sbjct: 20 LLVSLLL 26
>XP_006716282
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  LLVSLLL 7
          LLVSLLL
Sbjct: 55 LLVSLLL 61
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.149    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 383,931
Number of extensions: 468
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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