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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001073878                                                           17    2528
NP_001275685                                                           17    3301
NP_001028721                                                           17    3301
NP_054892                                                              17    3301
NP_037393                                                              16    5631
XP_005248191                                                           16    5631
XP_005248189                                                           16    5631
XP_005248188                                                           16    5631
XP_005248187                                                           16    5631
NP_112217                                                              16    5631
NP_060542                                                              16    7355
NP_003036                                                              16    7355
XP_005266564                                                           16    7355
NP_060225                                                              15    9605
NP_954657                                                              15    9605
NP_000215                                                              15    9605
NP_001271230                                                           15    9605
NP_001271231                                                           15    9605
NP_061155                                                              15    9605
NP_775819                                                              15    9605
XP_005258688                                                           15    9605
XP_005258687                                                           15    9605
XP_005258685                                                           15    9605
XP_005256993                                                           15    9605
XP_005256992                                                           15    9605
XP_006715810                                                           15    9605
XP_006715809                                                           15    9605
NP_001180384                                                           15    9605

>NP_001073878
          Length = 864

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LLNISQKME 9
           LLNISQ +E
Sbjct: 853 LLNISQPLE 861



>NP_001275685
          Length = 329

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LNISQKM 8
           LN+SQKM
Sbjct: 121 LNVSQKM 127



>NP_001028721
          Length = 329

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LNISQKM 8
           LN+SQKM
Sbjct: 121 LNVSQKM 127



>NP_054892
          Length = 329

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LNISQKM 8
           LN+SQKM
Sbjct: 121 LNVSQKM 127



>NP_037393
          Length = 798

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLNISQKME 9
           L+NISQ+++
Sbjct: 397 LINISQELQ 405



>XP_005248191
          Length = 702

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLNISQKME 9
           L+NISQ+++
Sbjct: 402 LINISQELQ 410



>XP_005248189
          Length = 795

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLNISQKME 9
           L+NISQ+++
Sbjct: 394 LINISQELQ 402



>XP_005248188
          Length = 802

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLNISQKME 9
           L+NISQ+++
Sbjct: 401 LINISQELQ 409



>XP_005248187
          Length = 803

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLNISQKME 9
           L+NISQ+++
Sbjct: 402 LINISQELQ 410



>NP_112217
          Length = 1594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LNISQKME 9
           +NISQK E
Sbjct: 214 VNISQKQE 221



>NP_060542
          Length = 2144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LLNISQKM 8
            LLNI QK+
Sbjct: 1259 LLNICQKL 1266



>NP_003036
          Length = 629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LLNISQKM 8
          LLNI Q+M
Sbjct: 6  LLNIGQQM 13



>XP_005266564
          Length = 629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LLNISQKM 8
          LLNI Q+M
Sbjct: 6  LLNIGQQM 13



>NP_060225
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLNISQKME 9
           LLN S+KM+
Sbjct: 571 LLNNSEKMK 579



>NP_954657
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LLNISQKME 9
           LLNI  K+E
Sbjct: 366 LLNIKVKLE 374



>NP_000215
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LLNISQKME 9
           LLNI  K+E
Sbjct: 366 LLNIKVKLE 374



>NP_001271230
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LNISQKME 9
           +NI QKM+
Sbjct: 677 VNIKQKMQ 684



>NP_001271231
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LNISQKME 9
           +NI QKM+
Sbjct: 676 VNIKQKMQ 683



>NP_061155
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LNISQKME 9
           +NI QKM+
Sbjct: 714 VNIKQKMQ 721



>NP_775819
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LLNISQK 7
          LLN++QK
Sbjct: 33 LLNVTQK 39



>XP_005258688
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LLNISQK 7
          LLN++QK
Sbjct: 25 LLNVTQK 31



>XP_005258687
          Length = 153

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LLNISQK 7
          LLN++QK
Sbjct: 33 LLNVTQK 39



>XP_005258685
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LLNISQK 7
          LLN++QK
Sbjct: 25 LLNVTQK 31



>XP_005256993
          Length = 1543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LLNISQKME 9
          L  +SQKME
Sbjct: 5  LQELSQKME 13



>XP_005256992
          Length = 1543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LLNISQKME 9
          L  +SQKME
Sbjct: 5  LQELSQKME 13



>XP_006715810
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LNISQKME 9
           +NI QKM+
Sbjct: 677 VNIKQKMQ 684



>XP_006715809
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LNISQKME 9
           +NI QKM+
Sbjct: 714 VNIKQKMQ 721



>NP_001180384
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLNISQKME 9
           LLN S+KM+
Sbjct: 536 LLNNSEKMK 544



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.127    0.294 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 393,152
Number of extensions: 680
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)