Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1482
XP_005271621                                                           18    1482
XP_006718907                                                           18    1482
XP_006718906                                                           18    1482
XP_005271619                                                           18    1482
XP_005271618                                                           18    1482
NP_002372                                                              16    5631
NP_036325                                                              16    5631
NP_998773                                                              16    5631
NP_002371                                                              16    5631
NP_085072                                                              16    5631
XP_005250977                                                           16    5631
NP_055568                                                              16    7355
NP_085095                                                              16    7355
NP_085080                                                              16    7355
NP_003824                                                              16    7355
XP_005260654                                                           16    7355
XP_005271906                                                           16    7355
XP_006718144                                                           16    7355
XP_005271905                                                           16    7355
XP_005271903                                                           16    7355
NP_003715                                                              15    9605
NP_001070995                                                           15    9605
NP_001266281                                                           15    9605
NP_006322                                                              15    9605
NP_219499                                                              15    9605
XP_006716742                                                           15    9605
XP_006716741                                                           15    9605
XP_006716740                                                           15    9605
XP_005250691                                                           15    9605
>NP_000042
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           LLMLIDSS
Sbjct: 927 LLMLIDSS 934
>XP_005271621
          Length = 2708

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           LLMLIDSS
Sbjct: 579 LLMLIDSS 586
>XP_006718907
          Length = 3050

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           LLMLIDSS
Sbjct: 927 LLMLIDSS 934
>XP_006718906
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           LLMLIDSS
Sbjct: 927 LLMLIDSS 934
>XP_005271619
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           LLMLIDSS
Sbjct: 927 LLMLIDSS 934
>XP_005271618
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           LLMLIDSS
Sbjct: 927 LLMLIDSS 934
>NP_002372
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 84 LVFIIDSSR 92
>NP_036325
          Length = 537

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LLMLIDSSR 9
           L  LIDSSR
Sbjct: 239 LTFLIDSSR 247
>NP_998773
          Length = 117

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 MLIDSSR 9
         M+IDSSR
Sbjct: 1 MIIDSSR 7
>NP_002371
          Length = 956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 58 LVFIIDSSR 66
>NP_085072
          Length = 937

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 58 LVFIIDSSR 66
>XP_005250977
          Length = 818

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 58 LVFIIDSSR 66
>NP_055568
          Length = 1282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           +LMLID+S
Sbjct: 150 VLMLIDAS 157
>NP_085095
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 35 LVFVIDSSR 43
>NP_085080
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 35 LVFVIDSSR 43
>NP_003824
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 35 LVFVIDSSR 43
>XP_005260654
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLMLIDSSR 9
          L+ +IDSSR
Sbjct: 35 LVFVIDSSR 43
>XP_005271906
          Length = 879

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           +LMLID+S
Sbjct: 150 VLMLIDAS 157
>XP_006718144
          Length = 886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           +LMLID+S
Sbjct: 150 VLMLIDAS 157
>XP_005271905
          Length = 1219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           +LMLID+S
Sbjct: 150 VLMLIDAS 157
>XP_005271903
          Length = 1282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLMLIDSS 8
           +LMLID+S
Sbjct: 150 VLMLIDAS 157
>NP_003715
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMLIDSSR 9
           ++L+DSSR
Sbjct: 295 VLLLDSSR 302
>NP_001070995
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMLIDSSR 9
           ++L+DSSR
Sbjct: 295 VLLLDSSR 302
>NP_001266281
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMLIDSSR 9
           ++L+DSSR
Sbjct: 295 VLLLDSSR 302
>NP_006322
          Length = 244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LLMLIDS 7
          LLML+DS
Sbjct: 92 LLMLMDS 98
>NP_219499
          Length = 1663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLMLIDSSR 9
           L ++IDSSR
Sbjct: 563 LKVIIDSSR 571
>XP_006716742
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMLIDSSR 9
           ++L+DSSR
Sbjct: 295 VLLLDSSR 302
>XP_006716741
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMLIDSSR 9
           ++L+DSSR
Sbjct: 295 VLLLDSSR 302
>XP_006716740
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LMLIDSSR 9
           ++L+DSSR
Sbjct: 295 VLLLDSSR 302
>XP_005250691
          Length = 1645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLMLIDSSR 9
           L ++IDSSR
Sbjct: 545 LKVIIDSSR 553
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.145    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 279,245
Number of extensions: 195
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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