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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1135
XP_006718908                                                           18    1135
XP_005271621                                                           18    1135
XP_006718907                                                           18    1135
XP_006718906                                                           18    1135
XP_005271619                                                           18    1135
XP_005271618                                                           18    1135
NP_775774                                                              17    4312
NP_001138405                                                           17    4312
NP_115646                                                              16    5631
XP_006711023                                                           16    5631
NP_001269961                                                           16    7355
NP_001269960                                                           16    7355
NP_001269962                                                           16    7355
NP_057190                                                              16    7355
NP_878255                                                              16    7355
NP_001244305                                                           16    7355
XP_005267526                                                           16    7355
NP_000581                                                              15    9605
NP_573566                                                              15    9605
XP_006711978                                                           15    9605

>NP_000042
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKSILEIIS 9
            LKSILEI+S
Sbjct: 1401 LKSILEILS 1409



>XP_006718908
          Length = 1708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  LKSILEIIS 9
          LKSILEI+S
Sbjct: 53 LKSILEILS 61



>XP_005271621
          Length = 2708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKSILEIIS 9
            LKSILEI+S
Sbjct: 1053 LKSILEILS 1061



>XP_006718907
          Length = 3050

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKSILEIIS 9
            LKSILEI+S
Sbjct: 1395 LKSILEILS 1403



>XP_006718906
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKSILEIIS 9
            LKSILEI+S
Sbjct: 1401 LKSILEILS 1409



>XP_005271619
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKSILEIIS 9
            LKSILEI+S
Sbjct: 1401 LKSILEILS 1409



>XP_005271618
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKSILEIIS 9
            LKSILEI+S
Sbjct: 1401 LKSILEILS 1409



>NP_775774
          Length = 438

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KSI+EI+S
Sbjct: 156 KSIIEIVS 163



>NP_001138405
          Length = 490

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KSI+EI+S
Sbjct: 208 KSIIEIVS 215



>NP_115646
          Length = 803

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSILEIIS 9
           LKSI EI+S
Sbjct: 624 LKSIEEIVS 632



>XP_006711023
          Length = 808

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSILEIIS 9
           LKSI EI+S
Sbjct: 629 LKSIEEIVS 637



>NP_001269961
          Length = 583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 377 KSLLDIIS 384



>NP_001269960
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 251 KSLLDIIS 258



>NP_001269962
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 251 KSLLDIIS 258



>NP_057190
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 436 KSLLDIIS 443



>NP_878255
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 369 KSLLDIIS 376



>NP_001244305
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 344 KSLLDIIS 351



>XP_005267526
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KSILEIIS 9
           KS+L+IIS
Sbjct: 411 KSLLDIIS 418



>NP_000581
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSILEI 7
           LKS+LEI
Sbjct: 125 LKSLLEI 131



>NP_573566
          Length = 1394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LKSILEII 8
            +KS+LE+I
Sbjct: 1304 VKSVLELI 1311



>XP_006711978
          Length = 1368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LKSILEII 8
            +KS+LE+I
Sbjct: 1278 VKSVLELI 1285



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.143    0.312 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,759
Number of extensions: 389
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)