Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              20     509
NP_001091680                                                           20     509
NP_001091679                                                           20     509
XP_006713048                                                           20     509
XP_006713047                                                           20     509
XP_006713046                                                           20     509
XP_005264943                                                           20     509
NP_000375                                                              18    1935
NP_075252                                                              17    2528
NP_998812                                                              17    2528
NP_002002                                                              17    2528
XP_005265895                                                           17    2528
XP_005265894                                                           17    2528
NP_060764                                                              16    5631
NP_005088                                                              16    7355
NP_056163                                                              16    7355
NP_064450                                                              16    7355
XP_006717822                                                           16    7355
XP_005263969                                                           16    7355
XP_005263968                                                           16    7355
XP_005263967                                                           16    7355
XP_005263966                                                           16    7355
XP_005273257                                                           16    7355
NP_009225                                                              15    9605
NP_009228                                                              15    9605
NP_009231                                                              15    9605
NP_001431                                                              15    9605
XP_006722099                                                           15    9605
XP_006722098                                                           15    9605
XP_006722097                                                           15    9605
XP_006722096                                                           15    9605
XP_006722095                                                           15    9605
XP_006722094                                                           15    9605
XP_006722093                                                           15    9605
XP_006722092                                                           15    9605
XP_006716365                                                           15    9605
>NP_001895
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 132 LKHAVVNLI 140
>NP_001091680
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 132 LKHAVVNLI 140
>NP_001091679
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 132 LKHAVVNLI 140
>XP_006713048
          Length = 720

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 132 LKHAVVNLI 140
>XP_006713047
          Length = 774

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 125 LKHAVVNLI 133
>XP_006713046
          Length = 774

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 125 LKHAVVNLI 133
>XP_005264943
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           LKH VVNLI
Sbjct: 132 LKHAVVNLI 140
>NP_000375
          Length = 4563

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LKHVVVNLI 9
            +KH V+NLI
Sbjct: 2317 VKHFVINLI 2325
>NP_075252
          Length = 762

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LKHVVVN 7
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>NP_998812
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LKHVVVN 7
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>NP_002002
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LKHVVVN 7
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>XP_005265895
          Length = 802

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LKHVVVN 7
           LKH+V+N
Sbjct: 284 LKHIVIN 290
>XP_005265894
          Length = 834

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   LKHVVVN 7
           LKH+V+N
Sbjct: 316 LKHIVIN 322
>NP_060764
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KHVVVNL 8
           KH+VVN+
Sbjct: 339 KHLVVNI 345
>NP_005088
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KHVVVNL 8
           KHV+V+L
Sbjct: 549 KHVIVDL 555
>NP_056163
          Length = 1883

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KHVVVNLI 9
           +H+++NLI
Sbjct: 922 RHLILNLI 929
>NP_064450
          Length = 1722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    LKHVVVNL 8
            ++H VVNL
Sbjct: 1382 IQHAVVNL 1389
>XP_006717822
          Length = 810

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   LKHVVVNLI 9
           +KH +VN++
Sbjct: 779 VKHQIVNVV 787
>XP_005263969
          Length = 1844

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KHVVVNLI 9
           +H+++NLI
Sbjct: 883 RHLILNLI 890
>XP_005263968
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KHVVVNLI 9
           +H+++NLI
Sbjct: 900 RHLILNLI 907
>XP_005263967
          Length = 1899

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KHVVVNLI 9
           +H+++NLI
Sbjct: 939 RHLILNLI 946
>XP_005263966
          Length = 1900

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KHVVVNLI 9
           +H+++NLI
Sbjct: 939 RHLILNLI 946
>XP_005273257
          Length = 1726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    LKHVVVNL 8
            ++H VVNL
Sbjct: 1386 IQHAVVNL 1393
>NP_009225
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>NP_009228
          Length = 1816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 427 LSHVTENLI 435
>NP_009231
          Length = 1884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>NP_001431
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   HVVVNL 8
           HV++NL
Sbjct: 273 HVIINL 278
>XP_006722099
          Length = 1736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 347 LSHVTENLI 355
>XP_006722098
          Length = 1793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 404 LSHVTENLI 412
>XP_006722097
          Length = 1862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>XP_006722096
          Length = 1862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>XP_006722095
          Length = 1862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 473 LSHVTENLI 481
>XP_006722094
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>XP_006722093
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>XP_006722092
          Length = 1863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKHVVVNLI 9
           L HV  NLI
Sbjct: 474 LSHVTENLI 482
>XP_006716365
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   HVVVNL 8
           HV++NL
Sbjct: 273 HVIINL 278
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.151    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 538,943
Number of extensions: 819
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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