Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              19     869
XP_006713721                                                           19     869
NP_116191                                                              17    2528
NP_003165                                                              17    2528
NP_068506                                                              17    2528
XP_005254781                                                           17    2528
XP_005254780                                                           17    2528
XP_005254779                                                           17    2528
XP_006717559                                                           17    2528
XP_006717558                                                           17    2528
XP_006717557                                                           17    2528
XP_006717556                                                           17    2528
XP_005252630                                                           17    2528
XP_005252629                                                           17    2528
XP_005252628                                                           17    2528
XP_005252627                                                           17    2528
XP_005252626                                                           17    2528
XP_005252625                                                           17    2528
XP_005252624                                                           17    2528
XP_005252623                                                           17    2528
XP_005252622                                                           17    2528
XP_006717555                                                           17    2528
XP_005252621                                                           17    2528
NP_056364                                                              17    4312
XP_005267570                                                           17    4312
XP_006720171                                                           17    4312
XP_005267569                                                           17    4312
XP_005267568                                                           17    4312
XP_006720170                                                           17    4312
XP_006720169                                                           17    4312
XP_006722597                                                           16    5631
XP_006722596                                                           16    5631
NP_002546                                                              15    9605
NP_899056                                                              15    9605
NP_001243275                                                           15    9605
NP_689558                                                              15    9605
NP_001026872                                                           15    9605
NP_001243277                                                           15    9605
NP_001243276                                                           15    9605
XP_006725191                                                           15    9605
XP_006725190                                                           15    9605
XP_005257977                                                           15    9605
XP_005253010                                                           15    9605
XP_006718306                                                           15    9605
XP_005253009                                                           15    9605
>NP_006209
          Length = 1068

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LKAISTRD 8
           LKAISTRD
Sbjct: 531 LKAISTRD 538
>XP_006713721
          Length = 1068

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   LKAISTRD 8
           LKAISTRD
Sbjct: 531 LKAISTRD 538
>NP_116191
          Length = 520

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LKAISTRDR 9
           LKA++T DR
Sbjct: 198 LKAVTTEDR 206
>NP_003165
          Length = 1788

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 405 KAISTRNR 412
>NP_068506
          Length = 2214

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 831 KAISTRNR 838
>XP_005254781
          Length = 452

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LKAISTRDR 9
           LKA++T DR
Sbjct: 198 LKAVTTEDR 206
>XP_005254780
          Length = 470

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LKAISTRDR 9
           LKA++T DR
Sbjct: 198 LKAVTTEDR 206
>XP_005254779
          Length = 538

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LKAISTRDR 9
           LKA++T DR
Sbjct: 198 LKAVTTEDR 206
>XP_006717559
          Length = 1866

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 483 KAISTRNR 490
>XP_006717558
          Length = 1908

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 483 KAISTRNR 490
>XP_006717557
          Length = 1940

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 515 KAISTRNR 522
>XP_006717556
          Length = 2024

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 599 KAISTRNR 606
>XP_005252630
          Length = 2256

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 831 KAISTRNR 838
>XP_005252629
          Length = 2256

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 831 KAISTRNR 838
>XP_005252628
          Length = 2256

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 831 KAISTRNR 838
>XP_005252627
          Length = 2256

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 831 KAISTRNR 838
>XP_005252626
          Length = 2260

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 877 KAISTRNR 884
>XP_005252625
          Length = 2277

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 852 KAISTRNR 859
>XP_005252624
          Length = 2292

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 909 KAISTRNR 916
>XP_005252623
          Length = 2302

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 877 KAISTRNR 884
>XP_005252622
          Length = 2308

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 909 KAISTRNR 916
>XP_006717555
          Length = 2328

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 903 KAISTRNR 910
>XP_005252621
          Length = 2334

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KAISTRDR 9
           KAISTR+R
Sbjct: 909 KAISTRNR 916
>NP_056364
          Length = 1163

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 652 ALSTRDR 658
>XP_005267570
          Length = 1219

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 708 ALSTRDR 714
>XP_006720171
          Length = 1240

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 729 ALSTRDR 735
>XP_005267569
          Length = 1340

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 829 ALSTRDR 835
>XP_005267568
          Length = 1340

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 829 ALSTRDR 835
>XP_006720170
          Length = 1354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 843 ALSTRDR 849
>XP_006720169
          Length = 1361

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AISTRDR 9
           A+STRDR
Sbjct: 850 ALSTRDR 856
>XP_006722597
          Length = 714

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LKAISTRDR 9
          L  +STRDR
Sbjct: 32 LSLLSTRDR 40
>XP_006722596
          Length = 718

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LKAISTRDR 9
          L  +STRDR
Sbjct: 32 LSLLSTRDR 40
>NP_002546
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 354 IKAVSTSD 361
>NP_899056
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 354 IKAVSTSD 361
>NP_001243275
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKAISTRDR 9
           LKA+ST  R
Sbjct: 499 LKALSTASR 507
>NP_689558
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KAISTRD 8
          +A+STRD
Sbjct: 87 EAVSTRD 93
>NP_001026872
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKAISTRDR 9
           LKA+ST  R
Sbjct: 499 LKALSTASR 507
>NP_001243277
          Length = 1057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKAISTRDR 9
           LKA+ST  R
Sbjct: 441 LKALSTASR 449
>NP_001243276
          Length = 1205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKAISTRDR 9
           LKA+ST  R
Sbjct: 553 LKALSTASR 561
>XP_006725191
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 211 IKAVSTSD 218
>XP_006725190
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 484 IKAVSTSD 491
>XP_005257977
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KAISTRD 8
          +A+STRD
Sbjct: 87 EAVSTRD 93
>XP_005253010
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 211 IKAVSTSD 218
>XP_006718306
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 354 IKAVSTSD 361
>XP_005253009
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKAISTRD 8
           +KA+ST D
Sbjct: 439 IKAVSTSD 446
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.130    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 332,499
Number of extensions: 411
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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