Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005301                                                              18    1135
NP_001229371                                                           18    1135
NP_001025173                                                           18    1135
NP_001229372                                                           18    1135
NP_722523                                                              16    5631
NP_065201                                                              16    5631
NP_647611                                                              16    5631
NP_647612                                                              16    5631
NP_002369                                                              16    5631
XP_005248797                                                           16    5631
XP_005248796                                                           16    5631
XP_005248795                                                           16    5631
XP_005247073                                                           16    5631
NP_066382                                                              16    7355
NP_064710                                                              16    7355
XP_005255493                                                           16    7355
XP_006721128                                                           16    7355
XP_005255492                                                           16    7355
NP_004374                                                              15    9605
NP_001120662                                                           15    9605
NP_056036                                                              15    9605
NP_001253989                                                           15    9605
NP_001226                                                              15    9605
NP_001253990                                                           15    9605
NP_001253988                                                           15    9605
NP_055563                                                              15    9605
NP_001193943                                                           15    9605
NP_940931                                                              15    9605
XP_006721943                                                           15    9605
XP_006721942                                                           15    9605
XP_005254225                                                           15    9605
XP_005254222                                                           15    9605
XP_006718792                                                           15    9605
XP_006718791                                                           15    9605
XP_006718790                                                           15    9605
XP_006717799                                                           15    9605
XP_006717798                                                           15    9605
NP_689666                                                              15    9605
>NP_005301
          Length = 532

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFLVREVQR 9
           LFLVRE QR
Sbjct: 454 LFLVRESQR 462
>NP_001229371
          Length = 555

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFLVREVQR 9
           LFLVRE QR
Sbjct: 477 LFLVRESQR 485
>NP_001025173
          Length = 532

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFLVREVQR 9
           LFLVRE QR
Sbjct: 454 LFLVRESQR 462
>NP_001229372
          Length = 532

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LFLVREVQR 9
           LFLVRE QR
Sbjct: 454 LFLVRESQR 462
>NP_722523
          Length = 946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           F+V E+QR
Sbjct: 414 FIVEEIQR 421
>NP_065201
          Length = 893

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           F+V E+QR
Sbjct: 370 FIVEEIQR 377
>NP_647611
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LFLVREVQR 9
           LFLVRE  R
Sbjct: 102 LFLVRESAR 110
>NP_647612
          Length = 507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LFLVREVQR 9
           LFLVRE  R
Sbjct: 143 LFLVRESAR 151
>NP_002369
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LFLVREVQR 9
           LFLVRE  R
Sbjct: 144 LFLVRESAR 152
>XP_005248797
          Length = 902

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           F+V E+QR
Sbjct: 370 FIVEEIQR 377
>XP_005248796
          Length = 937

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           F+V E+QR
Sbjct: 414 FIVEEIQR 421
>XP_005248795
          Length = 945

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           F+V E+QR
Sbjct: 413 FIVEEIQR 420
>XP_005247073
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LFLVREVQR 9
           LFL RE+ R
Sbjct: 667 LFLFREITR 675
>NP_066382
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LFLVREVQR 9
           LFL R++QR
Sbjct: 241 LFLDRDLQR 249
>NP_064710
          Length = 1052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LFLVREV 7
           LFLVRE+
Sbjct: 884 LFLVREL 890
>XP_005255493
          Length = 959

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LFLVREV 7
           LFLVRE+
Sbjct: 791 LFLVREL 797
>XP_006721128
          Length = 985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LFLVREV 7
           LFLVRE+
Sbjct: 817 LFLVREL 823
>XP_005255492
          Length = 1026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LFLVREV 7
           LFLVRE+
Sbjct: 858 LFLVREL 864
>NP_004374
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 103 LFLVRE 108
>NP_001120662
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 103 LFLVRE 108
>NP_056036
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           FL++ VQR
Sbjct: 918 FLIKPVQR 925
>NP_001253989
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 137 LFLVRE 142
>NP_001226
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFLVREVQ 8
           +FLVR+ Q
Sbjct: 389 IFLVRDTQ 396
>NP_001253990
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 142 LFLVRE 147
>NP_001253988
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 150 LFLVRE 155
>NP_055563
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 162 LFLVRE 167
>NP_001193943
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFLVREVQ 8
           +FLVR+ Q
Sbjct: 389 IFLVRDTQ 396
>NP_940931
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VREVQR 9
            VREVQR
Sbjct: 1317 VREVQR 1322
>XP_006721943
          Length = 1456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VREVQR 9
            VREVQR
Sbjct: 1317 VREVQR 1322
>XP_006721942
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VREVQR 9
            VREVQR
Sbjct: 1317 VREVQR 1322
>XP_005254225
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LFLVREVQR 9
           LFL REV +
Sbjct: 184 LFLEREVSK 192
>XP_005254222
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LFLVRE 6
           LFLVRE
Sbjct: 103 LFLVRE 108
>XP_006718792
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFLVREVQ 8
           +FLVR+ Q
Sbjct: 389 IFLVRDTQ 396
>XP_006718791
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFLVREVQ 8
           +FLVR+ Q
Sbjct: 389 IFLVRDTQ 396
>XP_006718790
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LFLVREVQ 8
           +FLVR+ Q
Sbjct: 389 IFLVRDTQ 396
>XP_006717799
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           FL++ VQR
Sbjct: 918 FLIKPVQR 925
>XP_006717798
          Length = 1534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FLVREVQR 9
           FL++ VQR
Sbjct: 918 FLIKPVQR 925
>NP_689666
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LFLVREVQR 9
           LFL REV +
Sbjct: 184 LFLEREVSK 192
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.158    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 428,967
Number of extensions: 694
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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