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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002027                                                              16    5631
NP_001116423                                                           16    5631
NP_001020461                                                           16    5631
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
NP_689733                                                              16    7355
NP_001106869                                                           16    7355
NP_001106868                                                           16    7355
NP_001269654                                                           16    7355
XP_005246888                                                           16    7355
XP_006712788                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
XP_006712387                                                           16    7355
XP_006712386                                                           16    7355
XP_006712385                                                           16    7355
XP_006712384                                                           16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_004095                                                              15    9605
NP_060250                                                              15    9605
NP_006174                                                              15    9605
NP_003794                                                              15    9605
NP_062830                                                              15    9605
XP_006716523                                                           15    9605
XP_006716522                                                           15    9605
XP_005251324                                                           15    9605
XP_005251323                                                           15    9605
NP_001138604                                                           15    9605

>NP_002027
          Length = 336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           E+KAL+AT
Sbjct: 202 ELKALQAT 209



>NP_001116423
          Length = 338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           E+KAL+AT
Sbjct: 204 ELKALQAT 211



>NP_001020461
          Length = 116

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LEVKALEAT 9
          LE+KALE++
Sbjct: 55 LELKALESS 63



>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 18850 QVKTLEAT 18857



>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 18975 QVKTLEAT 18982



>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 26274 QVKTLEAT 26281



>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 25347 QVKTLEAT 25354



>NP_689733
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           +VKAL+AT
Sbjct: 184 KVKALDAT 191



>NP_001106869
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  EVKALEAT 9
          +VKAL+AT
Sbjct: 82 KVKALDAT 89



>NP_001106868
          Length = 395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           +VKAL+AT
Sbjct: 108 KVKALDAT 115



>NP_001269654
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  EVKALEAT 9
          +VKAL+AT
Sbjct: 82 KVKALDAT 89



>XP_005246888
          Length = 22663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 14587 QVKTLEAT 14594



>XP_006712788
          Length = 24022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 15946 QVKTLEAT 15953



>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 25902 QVKTLEAT 25909



>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 27408 QVKTLEAT 27415



>XP_006712387
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           +VKAL+AT
Sbjct: 95  KVKALDAT 102



>XP_006712386
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           +VKAL+AT
Sbjct: 95  KVKALDAT 102



>XP_006712385
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           +VKAL+AT
Sbjct: 95  KVKALDAT 102



>XP_006712384
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EVKALEAT 9
           +VKAL+AT
Sbjct: 95  KVKALDAT 102



>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 27915 QVKTLEAT 27922



>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2     EVKALEAT 9
             +VK LEAT
Sbjct: 19042 QVKTLEAT 19049



>NP_004095
          Length = 2511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LEVKALEAT 9
           LEV+ LEA+
Sbjct: 933 LEVRLLEAS 941



>NP_060250
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EVKALEA 8
            EV+ALEA
Sbjct: 1947 EVRALEA 1953



>NP_006174
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  EVKALEA 8
          E+KALEA
Sbjct: 29 EMKALEA 35



>NP_003794
          Length = 1685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LEVKALEA 8
           L+VK LEA
Sbjct: 514 LDVKCLEA 521



>NP_062830
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LEVKALEA 8
           L+VK LEA
Sbjct: 514 LDVKCLEA 521



>XP_006716523
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EVKALEA 8
           EV+ALEA
Sbjct: 862 EVRALEA 868



>XP_006716522
          Length = 2192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EVKALEA 8
            EV+ALEA
Sbjct: 1142 EVRALEA 1148



>XP_005251324
          Length = 2996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EVKALEA 8
            EV+ALEA
Sbjct: 1947 EVRALEA 1953



>XP_005251323
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    EVKALEA 8
            EV+ALEA
Sbjct: 1947 EVRALEA 1953



>NP_001138604
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LEVKALEAT 9
           +EV ALE T
Sbjct: 106 MEVSALEKT 114



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.118    0.264 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 280,484
Number of extensions: 234
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)