Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              20     390
NP_848566                                                              18    1935
NP_001119580                                                           16    5631
NP_000330                                                              16    5631
NP_001119579                                                           16    5631
XP_005256176                                                           16    5631
NP_006022                                                              16    7355
NP_000951                                                              16    7355
NP_055030                                                              16    7355
XP_005261186                                                           16    7355
XP_005261185                                                           16    7355
XP_005261184                                                           16    7355
XP_005261183                                                           16    7355
XP_005261182                                                           16    7355
XP_005261181                                                           16    7355
XP_005259152                                                           16    7355
XP_005259150                                                           16    7355
NP_068567                                                              16    7355
NP_001121121                                                           15    9605
NP_060399                                                              15    9605
NP_056207                                                              15    9605
NP_054828                                                              15    9605
NP_078788                                                              15    9605
NP_005348                                                              15    9605
XP_005258998                                                           15    9605
XP_006723281                                                           15    9605
XP_005258997                                                           15    9605
XP_005258996                                                           15    9605
XP_005258995                                                           15    9605
XP_005258994                                                           15    9605
XP_006719412                                                           15    9605
NP_683762                                                              15    9605
>NP_005350
          Length = 552

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LCILRMRFV 9
           LCILRM FV
Sbjct: 498 LCILRMSFV 506
>NP_848566
          Length = 335

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  LCILRMRFV 9
          LC LRM FV
Sbjct: 77 LCSLRMAFV 85
>NP_001119580
          Length = 1021

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   LCILRMR 7
           +C++RMR
Sbjct: 771 VCVMRMR 777
>NP_000330
          Length = 1030

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   LCILRMR 7
           +C++RMR
Sbjct: 771 VCVMRMR 777
>NP_001119579
          Length = 1029

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   LCILRMR 7
           +C++RMR
Sbjct: 770 VCVMRMR 776
>XP_005256176
          Length = 1020

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   LCILRMR 7
           +C++RMR
Sbjct: 770 VCVMRMR 776
>NP_006022
          Length = 3336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3204 IAILRLRFL 3212
>NP_000951
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CILRMRF 8
           C LRMR+
Sbjct: 170 CFLRMRW 176
>NP_055030
          Length = 1297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   LCILRMRF 8
           LC+ R+R+
Sbjct: 863 LCVSRLRY 870
>XP_005261186
          Length = 3139

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3007 IAILRLRFL 3015
>XP_005261185
          Length = 3229

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3097 IAILRLRFL 3105
>XP_005261184
          Length = 3258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3215 IAILRLRFL 3223
>XP_005261183
          Length = 3268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3136 IAILRLRFL 3144
>XP_005261182
          Length = 3346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3214 IAILRLRFL 3222
>XP_005261181
          Length = 3347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LCILRMRFV 9
            + ILR+RF+
Sbjct: 3215 IAILRLRFL 3223
>XP_005259152
          Length = 257

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CILRMRF 8
           C LRMR+
Sbjct: 170 CFLRMRW 176
>XP_005259150
          Length = 342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CILRMRF 8
           C LRMR+
Sbjct: 170 CFLRMRW 176
>NP_068567
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           +C L +RFV
Sbjct: 388 VCFLLLRFV 396
>NP_001121121
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LCILRMRF 8
           LC+L +RF
Sbjct: 262 LCLLVVRF 269
>NP_060399
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LCILRMRF 8
           LC+L +RF
Sbjct: 262 LCLLVVRF 269
>NP_056207
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  LCILRMR 7
          LC+ RMR
Sbjct: 77 LCVPRMR 83
>NP_054828
          Length = 1390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    LCILRM 6
            +C+LRM
Sbjct: 1291 MCVLRM 1296
>NP_078788
          Length = 371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   LCILRM 6
           +C+LRM
Sbjct: 231 ICVLRM 236
>NP_005348
          Length = 1076

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LCILRMRFV 9
            LC+ R+R V
Sbjct: 1042 LCVERIRLV 1050
>XP_005258998
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           LC+ R+R V
Sbjct: 741 LCVERIRLV 749
>XP_006723281
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           LC+ R+R V
Sbjct: 741 LCVERIRLV 749
>XP_005258997
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           LC+ R+R V
Sbjct: 741 LCVERIRLV 749
>XP_005258996
          Length = 786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           LC+ R+R V
Sbjct: 752 LCVERIRLV 760
>XP_005258995
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           LC+ R+R V
Sbjct: 787 LCVERIRLV 795
>XP_005258994
          Length = 1000

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LCILRMRFV 9
           LC+ R+R V
Sbjct: 966 LCVERIRLV 974
>XP_006719412
          Length = 724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 7/8 (87%)

Query: 2   CILRMRFV 9
           C++R +F+
Sbjct: 704 CVVRTKFI 711
>NP_683762
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  LCILRMR 7
          +C+LR+R
Sbjct: 29 VCVLRVR 35
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.369    0.167    0.577 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 453,793
Number of extensions: 549
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.5 bits)
S2: 28 (15.4 bits)

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