Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           20     390
NP_000240                                                              20     390
NP_001161089                                                           20     390
XP_005265218                                                           20     390
NP_004629                                                              17    3301
NP_542417                                                              17    3301
XP_006726175                                                           17    3301
XP_006726174                                                           17    3301
XP_006726087                                                           17    3301
XP_006726086                                                           17    3301
XP_006725985                                                           17    3301
XP_006725897                                                           17    3301
XP_006725896                                                           17    3301
XP_006725787                                                           17    3301
XP_006725786                                                           17    3301
XP_006725571                                                           17    3301
XP_006725570                                                           17    3301
XP_006715254                                                           17    3301
NP_057336                                                              16    5631
NP_001254513                                                           16    5631
NP_055763                                                              16    5631
NP_001252541                                                           16    5631
NP_057368                                                              16    5631
NP_955447                                                              16    5631
XP_005263859                                                           16    5631
XP_006712120                                                           16    5631
XP_005264512                                                           16    5631
XP_005264511                                                           16    5631
XP_006712119                                                           16    5631
XP_005264510                                                           16    5631
XP_006712118                                                           16    5631
XP_005264509                                                           16    5631
XP_005264508                                                           16    5631
XP_006712117                                                           16    5631
XP_005264507                                                           16    5631
XP_005264506                                                           16    5631
NP_060848                                                              16    5631
NP_751898                                                              16    7355
NP_002432                                                              16    7355
NP_751897                                                              16    7355
NP_068744                                                              16    7355
NP_001269652                                                           16    7355
NP_001121186                                                           16    7355
NP_078779                                                              16    7355
NP_689753                                                              16    7355
XP_006710872                                                           16    7355
XP_006710871                                                           16    7355
NP_078780                                                              15    9605
NP_001073896                                                           15    9605
NP_001129494                                                           15    9605
NP_001185896                                                           15    9605
NP_996882                                                              15    9605
NP_064706                                                              15    9605
>NP_001245200
          Length = 687

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LASISHAAH 9
           LASISH AH
Sbjct: 104 LASISHVAH 112
>NP_000240
          Length = 756

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LASISHAAH 9
           LASISH AH
Sbjct: 104 LASISHVAH 112
>NP_001161089
          Length = 658

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LASISHAAH 9
          LASISH AH
Sbjct: 6  LASISHVAH 14
>XP_005265218
          Length = 687

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LASISHAAH 9
           LASISH AH
Sbjct: 104 LASISHVAH 112
>NP_004629
          Length = 2157

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>NP_542417
          Length = 2157

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006726175
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006726174
          Length = 2156

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006726087
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006726086
          Length = 2156

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725985
          Length = 2156

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725897
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725896
          Length = 2156

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725787
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725786
          Length = 2156

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725571
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006725570
          Length = 2156

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>XP_006715254
          Length = 2086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           AS++H AH
Sbjct: 145 ASVTHGAH 152
>NP_057336
          Length = 4857

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1762 AISHASH 1768
>NP_001254513
          Length = 512

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SISHAAH 9
           S+SH AH
Sbjct: 166 SLSHVAH 172
>NP_055763
          Length = 541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SISHAAH 9
           S+SH AH
Sbjct: 166 SLSHVAH 172
>NP_001252541
          Length = 2371

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    LASISHAAH 9
            +++I+H+AH
Sbjct: 2234 MSTITHSAH 2242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LASISH 6
           LASISH
Sbjct: 970 LASISH 975
>NP_057368
          Length = 2376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    LASISHAAH 9
            +++I+H+AH
Sbjct: 2239 MSTITHSAH 2247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LASISH 6
           LASISH
Sbjct: 975 LASISH 980
>NP_955447
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SISHAAH 9
           S+SH AH
Sbjct: 166 SLSHVAH 172
>XP_005263859
          Length = 1606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  ISHAAH 9
          +SHAAH
Sbjct: 28 LSHAAH 33

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SISHAA 8
            SISHAA
Sbjct: 1336 SISHAA 1341
>XP_006712120
          Length = 4812

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1780 AISHASH 1786
>XP_005264512
          Length = 4840

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1736 AISHASH 1742
>XP_005264511
          Length = 4847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1780 AISHASH 1786
>XP_006712119
          Length = 4851

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1747 AISHASH 1753
>XP_005264510
          Length = 4861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1766 AISHASH 1772
>XP_006712118
          Length = 4864

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1780 AISHASH 1786
>XP_005264509
          Length = 4870

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1766 AISHASH 1772
>XP_005264508
          Length = 4875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1780 AISHASH 1786
>XP_006712117
          Length = 4878

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1780 AISHASH 1786
>XP_005264507
          Length = 4880

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1776 AISHASH 1782
>XP_005264506
          Length = 4884

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            +ISHA+H
Sbjct: 1780 AISHASH 1786
>NP_060848
          Length = 374

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LASISHA 7
           LAS+SHA
Sbjct: 200 LASLSHA 206
>NP_751898
          Length = 834

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LASISHAAH 9
           +A  SHA+H
Sbjct: 748 VAKASHASH 756
>NP_002432
          Length = 834

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LASISHAAH 9
           +A  SHA+H
Sbjct: 748 VAKASHASH 756
>NP_751897
          Length = 835

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LASISHAAH 9
           +A  SHA+H
Sbjct: 749 VAKASHASH 757
>NP_068744
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SISHAAH 9
          +ISHA H
Sbjct: 79 TISHAGH 85
>NP_001269652
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SISHAAH 9
          +ISHA H
Sbjct: 79 TISHAGH 85
>NP_001121186
          Length = 2405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            ++SH+AH
Sbjct: 998  NVSHSAH 1004
>NP_078779
          Length = 2406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            ++SH+AH
Sbjct: 998  NVSHSAH 1004
>NP_689753
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   LASISHAAH 9
           + ++SHA H
Sbjct: 104 VTTVSHAVH 112
>XP_006710872
          Length = 1590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            ++SH+AH
Sbjct: 998  NVSHSAH 1004
>XP_006710871
          Length = 2406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    SISHAAH 9
            ++SH+AH
Sbjct: 998  NVSHSAH 1004
>NP_078780
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   SHAAH 9
           SHAAH
Sbjct: 528 SHAAH 532
>NP_001073896
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SISHAA 8
            SISHAA
Sbjct: 1307 SISHAA 1312
>NP_001129494
          Length = 964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LASISH 6
          LASISH
Sbjct: 13 LASISH 18
>NP_001185896
          Length = 857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LASISH 6
          LASISH
Sbjct: 13 LASISH 18
>NP_996882
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LASISH 6
           LASISH
Sbjct: 975 LASISH 980
>NP_064706
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   ASISHAAH 9
           A+++H AH
Sbjct: 496 ATLNHVAH 503
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.114    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 564,383
Number of extensions: 813
Number of successful extensions: 56
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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