Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           20     509
NP_060785                                                              20     509
NP_361014                                                              20     509
NP_510966                                                              16    5631
NP_001775                                                              16    5631
NP_001020331                                                           16    5631
NP_055747                                                              16    5631
NP_001137419                                                           16    5631
NP_061847                                                              16    5631
XP_005260229                                                           16    5631
XP_005260228                                                           16    5631
XP_005260227                                                           16    5631
XP_005247294                                                           16    5631
XP_005247293                                                           16    5631
XP_005247292                                                           16    5631
XP_005247291                                                           16    5631
XP_005247290                                                           16    5631
XP_006713603                                                           16    5631
XP_005247289                                                           16    5631
NP_001153468                                                           16    7355
NP_001153471                                                           16    7355
NP_001153467                                                           16    7355
NP_039254                                                              16    7355
NP_001153480                                                           16    7355
NP_039250                                                              16    7355
NP_001153473                                                           16    7355
NP_001153476                                                           16    7355
NP_039258                                                              16    7355
NP_039251                                                              16    7355
NP_001127878                                                           16    7355
NP_001127877                                                           16    7355
NP_612147                                                              16    7355
XP_006716398                                                           16    7355
XP_005273544                                                           16    7355
XP_005273543                                                           16    7355
XP_005273542                                                           16    7355
XP_005250215                                                           16    7355
XP_005250214                                                           16    7355
XP_005250213                                                           16    7355
NP_001177203                                                           15    9605
NP_079409                                                              15    9605
NP_055820                                                              15    9605
XP_005267607                                                           15    9605
XP_005264631                                                           15    9605
XP_005264630                                                           15    9605
XP_005264629                                                           15    9605
>NP_001013433
          Length = 589

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  KVSEYTST 8
          KVSEYTST
Sbjct: 71 KVSEYTST 78
>NP_060785
          Length = 627

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KVSEYTST 8
           KVSEYTST
Sbjct: 109 KVSEYTST 116
>NP_361014
          Length = 707

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KVSEYTST 8
           KVSEYTST
Sbjct: 189 KVSEYTST 196
>NP_510966
          Length = 835

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEYTST 8
           K SE+TST
Sbjct: 809 KYSEFTST 816
>NP_001775
          Length = 742

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEYTST 8
           K SE+TST
Sbjct: 716 KYSEFTST 723
>NP_001020331
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEYTST 8
           K SE+TST
Sbjct: 760 KYSEFTST 767
>NP_055747
          Length = 823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 619 ISQYTST 625
>NP_001137419
          Length = 415

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEYTSTA 9
           KVS+YT  A
Sbjct: 270 KVSQYTGRA 278
>NP_061847
          Length = 457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KVSEYTSTA 9
           KVS+YT  A
Sbjct: 312 KVSQYTGRA 320
>XP_005260229
          Length = 801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEYTST 8
           K SE+TST
Sbjct: 775 KYSEFTST 782
>XP_005260228
          Length = 845

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEYTST 8
           K SE+TST
Sbjct: 819 KYSEFTST 826
>XP_005260227
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KVSEYTST 8
           K SE+TST
Sbjct: 868 KYSEFTST 875
>XP_005247294
          Length = 671

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 467 ISQYTST 473
>XP_005247293
          Length = 814

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 610 ISQYTST 616
>XP_005247292
          Length = 823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 619 ISQYTST 625
>XP_005247291
          Length = 823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 619 ISQYTST 625
>XP_005247290
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 630 ISQYTST 636
>XP_006713603
          Length = 843

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 639 ISQYTST 645
>XP_005247289
          Length = 843

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSEYTST 8
           +S+YTST
Sbjct: 639 ISQYTST 645
>NP_001153468
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 181 SHYTSTA 187
>NP_001153471
          Length = 624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 319 SHYTSTA 325
>NP_001153467
          Length = 607

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 302 SHYTSTA 308
>NP_039254
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 335 SHYTSTA 341
>NP_001153480
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 332 SHYTSTA 338
>NP_039250
          Length = 645

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 340 SHYTSTA 346
>NP_001153473
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 285 SHYTSTA 291
>NP_001153476
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 332 SHYTSTA 338
>NP_039258
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 335 SHYTSTA 341
>NP_039251
          Length = 637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 332 SHYTSTA 338
>NP_001127878
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSEYTSTA 9
           +SEY STA
Sbjct: 138 LSEYISTA 145
>NP_001127877
          Length = 456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSEYTSTA 9
           +SEY STA
Sbjct: 138 LSEYISTA 145
>NP_612147
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSEYTSTA 9
           +SEY STA
Sbjct: 155 LSEYISTA 162
>XP_006716398
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 115 SHYTSTA 121
>XP_005273544
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 387 SHYTSTA 393
>XP_005273543
          Length = 695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 390 SHYTSTA 396
>XP_005273542
          Length = 700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SEYTSTA 9
           S YTSTA
Sbjct: 395 SHYTSTA 401
>XP_005250215
          Length = 336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VSEYTSTA 9
          +SEY STA
Sbjct: 18 LSEYISTA 25
>XP_005250214
          Length = 342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VSEYTSTA 9
          +SEY STA
Sbjct: 24 LSEYISTA 31
>XP_005250213
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSEYTSTA 9
           +SEY STA
Sbjct: 138 LSEYISTA 145
>NP_001177203
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KVSEYTS 7
           K+S YTS
Sbjct: 838 KISSYTS 844
>NP_079409
          Length = 843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KVSEYTS 7
           K+S YTS
Sbjct: 754 KISSYTS 760
>NP_055820
          Length = 1519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SEYTSTA 9
            S+YTST+
Sbjct: 1185 SDYTSTS 1191
>XP_005267607
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SEYTSTA 9
            S+YTST+
Sbjct: 1255 SDYTSTS 1261
>XP_005264631
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KVSEYTS 7
           K+S YTS
Sbjct: 837 KISSYTS 843
>XP_005264630
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KVSEYTS 7
           K+S YTS
Sbjct: 837 KISSYTS 843
>XP_005264629
          Length = 954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KVSEYTS 7
           K+S YTS
Sbjct: 838 KISSYTS 844
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.286   0.0974    0.224 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 399,535
Number of extensions: 241
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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