Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001185761                                                           22     134
NP_055459                                                              22     134
XP_006726410                                                           22     134
XP_006711719                                                           22     134
XP_006711716                                                           22     134
XP_005273038                                                           22     134
XP_006711164                                                           22     134
NP_004474                                                              18    1935
XP_003960941                                                           18    1935
NP_056477                                                              17    2528
XP_006712050                                                           17    2528
XP_005264311                                                           17    2528
XP_006712049                                                           17    2528
NP_005520                                                              17    3301
NP_006728                                                              17    3301
NP_001229796                                                           17    3301
NP_076991                                                              17    3301
XP_006710661                                                           17    3301
XP_005245920                                                           17    3301
XP_006710660                                                           17    3301
XP_006710659                                                           17    3301
XP_006710658                                                           17    3301
XP_006710657                                                           17    3301
NP_065883                                                              16    5631
NP_060382                                                              16    5631
NP_258260                                                              16    5631
NP_001158608                                                           16    5631
XP_006714867                                                           16    5631
XP_006714866                                                           16    5631
XP_005268581                                                           16    5631
XP_005263140                                                           16    5631
XP_005245423                                                           16    5631
XP_005245425                                                           16    5631
NP_001697                                                              16    7355
NP_001128210                                                           16    7355
NP_001124317                                                           16    7355
NP_001157915                                                           16    7355
NP_114130                                                              16    7355
XP_005267210                                                           16    7355
XP_005247751                                                           16    7355
NP_036581                                                              15    9605
NP_006370                                                              15    9605
NP_064581                                                              15    9605
XP_005250170                                                           15    9605
>NP_001185761
          Length = 2240

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2229 KSLRALPCT 2237
>NP_055459
          Length = 2346

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2335 KSLRALPCT 2343
>XP_006726410
          Length = 2087

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2076 KSLRALPCT 2084
>XP_006711719
          Length = 2302

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2291 KSLRALPCT 2299
>XP_006711716
          Length = 2411

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2400 KSLRALPCT 2408
>XP_005273038
          Length = 2509

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2498 KSLRALPCT 2506
>XP_006711164
          Length = 2087

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSVRALPCT 9
            KS+RALPCT
Sbjct: 2076 KSLRALPCT 2084
>NP_004474
          Length = 173

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KSVRALPCT 9
          +SVRAL CT
Sbjct: 7  RSVRALLCT 15
>XP_003960941
          Length = 173

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KSVRALPCT 9
          +SVRAL CT
Sbjct: 7  RSVRALLCT 15
>NP_056477
          Length = 1749

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VRALPC 8
            VRALPC
Sbjct: 1681 VRALPC 1686
>XP_006712050
          Length = 1716

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VRALPC 8
            VRALPC
Sbjct: 1648 VRALPC 1653
>XP_005264311
          Length = 1727

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VRALPC 8
            VRALPC
Sbjct: 1659 VRALPC 1664
>XP_006712049
          Length = 1728

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VRALPC 8
            VRALPC
Sbjct: 1660 VRALPC 1665
>NP_005520
          Length = 4391

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 279 SVRPLPC 285
>NP_006728
          Length = 455

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           S RALPC
Sbjct: 296 SFRALPC 302
>NP_001229796
          Length = 438

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           S RALPC
Sbjct: 296 SFRALPC 302
>NP_076991
          Length = 562

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KSVRALPCT 9
          ++VRAL CT
Sbjct: 50 EAVRALTCT 58
>XP_006710661
          Length = 4057

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 296 SVRPLPC 302
>XP_005245920
          Length = 4392

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 279 SVRPLPC 285
>XP_006710660
          Length = 4397

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 279 SVRPLPC 285
>XP_006710659
          Length = 4556

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 296 SVRPLPC 302
>XP_006710658
          Length = 4563

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 279 SVRPLPC 285
>XP_006710657
          Length = 4579

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           SVR LPC
Sbjct: 296 SVRPLPC 302
>NP_065883
          Length = 1237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   KSVRALPC 8
           KSV+  PC
Sbjct: 988 KSVKKFPC 995
>NP_060382
          Length = 1022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRALPC 8
           +RALPC
Sbjct: 466 LRALPC 471
>NP_258260
          Length = 690

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           + RALPC
Sbjct: 465 ATRALPC 471
>NP_001158608
          Length = 986

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRALPC 8
           +RALPC
Sbjct: 466 LRALPC 471
>XP_006714867
          Length = 632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           + RALPC
Sbjct: 465 ATRALPC 471
>XP_006714866
          Length = 647

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           + RALPC
Sbjct: 422 ATRALPC 428
>XP_005268581
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SVRALPC 8
           + RALPC
Sbjct: 463 ATRALPC 469
>XP_005263140
          Length = 1034

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VRALPC 8
           +RALPC
Sbjct: 478 LRALPC 483
>XP_005245423
          Length = 1246

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    KSVRALPC 8
            KSV+  PC
Sbjct: 997  KSVKKFPC 1004
>XP_005245425
          Length = 1299

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    KSVRALPC 8
            KSV+  PC
Sbjct: 1050 KSVKKFPC 1057
>NP_001697
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RALPC 8
           RALPC
Sbjct: 228 RALPC 232
>NP_001128210
          Length = 650

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RALPC 8
           RALPC
Sbjct: 228 RALPC 232
>NP_001124317
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RALPC 8
           RALPC
Sbjct: 228 RALPC 232
>NP_001157915
          Length = 1873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    KSVRALPC 8
            KSV  +PC
Sbjct: 1657 KSVSVVPC 1664
>NP_114130
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RALPC 8
           RALPC
Sbjct: 165 RALPC 169
>XP_005267210
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RALPC 8
           RALPC
Sbjct: 165 RALPC 169
>XP_005247751
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RALPC 8
           RALPC
Sbjct: 228 RALPC 232
>NP_036581
          Length = 339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   KSVRALPC 8
           KS+  LPC
Sbjct: 305 KSILFLPC 312
>NP_006370
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVRALP 7
           SVRALP
Sbjct: 682 SVRALP 687
>NP_064581
          Length = 855

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSVRALPCT 9
           +++RALP T
Sbjct: 135 RALRALPIT 143
>XP_005250170
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SVRALP 7
           SVRALP
Sbjct: 624 SVRALP 629
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.128    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 525,321
Number of extensions: 592
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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