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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_116214                                                              17    3301
NP_001243767                                                           17    3301
XP_005269218                                                           16    5631
NP_114432                                                              16    7355
NP_056290                                                              16    7355
XP_006726461                                                           16    7355
NP_061971                                                              15    9605
NP_001032370                                                           15    9605
NP_443749                                                              15    9605

>NP_116214
          Length = 550

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSIRQKTAI 9
           K+ RQKTA+
Sbjct: 331 KAFRQKTAL 339



>NP_001243767
          Length = 549

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSIRQKTAI 9
           K+ RQKTA+
Sbjct: 330 KAFRQKTAL 338



>XP_005269218
          Length = 2009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SIRQKTAI 9
          S+RQK+AI
Sbjct: 5  SLRQKSAI 12



>NP_114432
          Length = 1249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSIRQKTAI 9
           K  RQKTA+
Sbjct: 578 KRSRQKTAV 586



>NP_056290
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIRQKTA 8
           SIR+KTA
Sbjct: 285 SIREKTA 291



>XP_006726461
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KSIRQKTA 8
           +++RQKTA
Sbjct: 273 RTLRQKTA 280



>NP_061971
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KSIRQKTAI 9
           K +RQ+TA+
Sbjct: 137 KRMRQRTAL 145



>NP_001032370
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KSIRQKT 7
           KS+R+KT
Sbjct: 317 KSLREKT 323



>NP_443749
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KSIRQKT 7
           KS+R+KT
Sbjct: 349 KSLREKT 355



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.121    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,625
Number of extensions: 423
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)