Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              19     869
XP_006718908                                                           19     869
XP_005271621                                                           19     869
XP_006718907                                                           19     869
XP_006718906                                                           19     869
XP_005271619                                                           19     869
XP_005271618                                                           19     869
NP_006538                                                              17    4312
NP_775774                                                              17    4312
XP_006715702                                                           17    4312
XP_006715701                                                           17    4312
NP_001138405                                                           17    4312
NP_775859                                                              16    5631
NP_000581                                                              16    7355
NP_644671                                                              16    7355
NP_009330                                                              16    7355
NP_000405                                                              16    7355
NP_001186221                                                           16    7355
NP_001186220                                                           16    7355
NP_001269961                                                           16    7355
NP_001269960                                                           16    7355
NP_001269962                                                           16    7355
NP_057190                                                              16    7355
NP_878255                                                              16    7355
NP_001244305                                                           16    7355
XP_005267526                                                           16    7355
XP_006712781                                                           16    7355
NP_006493                                                              15    9605
NP_001254514                                                           15    9605
NP_001254515                                                           15    9605
XP_006721485                                                           15    9605
XP_006721484                                                           15    9605
XP_006721483                                                           15    9605
XP_006715181                                                           15    9605
XP_005249243                                                           15    9605
>NP_000042
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSILEIISK 9
            KSILEI+SK
Sbjct: 1402 KSILEILSK 1410
>XP_006718908
          Length = 1708

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  KSILEIISK 9
          KSILEI+SK
Sbjct: 54 KSILEILSK 62
>XP_005271621
          Length = 2708

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSILEIISK 9
            KSILEI+SK
Sbjct: 1054 KSILEILSK 1062
>XP_006718907
          Length = 3050

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSILEIISK 9
            KSILEI+SK
Sbjct: 1396 KSILEILSK 1404
>XP_006718906
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSILEIISK 9
            KSILEI+SK
Sbjct: 1402 KSILEILSK 1410
>XP_005271619
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSILEIISK 9
            KSILEI+SK
Sbjct: 1402 KSILEILSK 1410
>XP_005271618
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KSILEIISK 9
            KSILEI+SK
Sbjct: 1402 KSILEILSK 1410
>NP_006538
          Length = 579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           KSILEI+ K
Sbjct: 258 KSILEIMHK 266
>NP_775774
          Length = 438

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KSI+EI+S
Sbjct: 156 KSIIEIVS 163
>XP_006715702
          Length = 422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           KSILEI+ K
Sbjct: 101 KSILEIMHK 109
>XP_006715701
          Length = 527

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           KSILEI+ K
Sbjct: 258 KSILEIMHK 266
>NP_001138405
          Length = 490

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KSI+EI+S
Sbjct: 208 KSIIEIVS 215
>NP_775859
          Length = 1326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           K +LEI+SK
Sbjct: 189 KEMLEILSK 197
>NP_000581
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KSILEIISK 9
           KS+LEI  K
Sbjct: 126 KSLLEIFQK 134
>NP_644671
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           +SILE+I K
Sbjct: 559 ESILELIKK 567
>NP_009330
          Length = 750

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           +SILE+I K
Sbjct: 559 ESILELIKK 567
>NP_000405
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           SI+E++SK
Sbjct: 294 SIIEVLSK 301
>NP_001186221
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           SI+E++SK
Sbjct: 276 SIIEVLSK 283
>NP_001186220
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           SI+E++SK
Sbjct: 319 SIIEVLSK 326
>NP_001269961
          Length = 583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 377 KSLLDIIS 384
>NP_001269960
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 251 KSLLDIIS 258
>NP_001269962
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 251 KSLLDIIS 258
>NP_057190
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 436 KSLLDIIS 443
>NP_878255
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 369 KSLLDIIS 376
>NP_001244305
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 344 KSLLDIIS 351
>XP_005267526
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KSILEIIS 8
           KS+L+IIS
Sbjct: 411 KSLLDIIS 418
>XP_006712781
          Length = 750

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KSILEIISK 9
           +SILE+I K
Sbjct: 559 ESILELIKK 567
>NP_006493
          Length = 713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KSILEIISK 9
           KS+LE+  K
Sbjct: 611 KSVLEVTQK 619
>NP_001254514
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           S++EI+SK
Sbjct: 711 SLMEILSK 718
>NP_001254515
          Length = 999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           S++EI+SK
Sbjct: 658 SLMEILSK 665
>XP_006721485
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           S++EI+SK
Sbjct: 711 SLMEILSK 718
>XP_006721484
          Length = 997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           S++EI+SK
Sbjct: 656 SLMEILSK 663
>XP_006721483
          Length = 1061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SILEIISK 9
           S++EI+SK
Sbjct: 740 SLMEILSK 747
>XP_006715181
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KSILEIISK 9
           KS+LE+  K
Sbjct: 459 KSVLEVTQK 467
>XP_005249243
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KSILEIISK 9
           KS+LE+  K
Sbjct: 549 KSVLEVTQK 557
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.133    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 363,876
Number of extensions: 456
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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