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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              19     869
XP_005268094                                                           19     869
NP_852111                                                              18    1935
NP_000136                                                              18    1935
NP_000224                                                              17    2528
XP_005264366                                                           17    2528
XP_006712078                                                           17    2528
XP_005274043                                                           16    7355
XP_006718611                                                           16    7355
XP_006718610                                                           16    7355
NP_001034841                                                           16    7355
NP_068587                                                              15    9605

>NP_000360
          Length = 764

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           KRMAVLIF
Sbjct: 624 KRMAVLIF 631



>XP_005268094
          Length = 764

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           KRMAVLIF
Sbjct: 624 KRMAVLIF 631



>NP_852111
          Length = 669

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           KRMA+LIF
Sbjct: 546 KRMAMLIF 553



>NP_000136
          Length = 695

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           KRMA+LIF
Sbjct: 572 KRMAMLIF 579



>NP_000224
          Length = 699

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           K+MA+LIF
Sbjct: 569 KKMAILIF 576



>XP_005264366
          Length = 380

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           K+MA+LIF
Sbjct: 250 KKMAILIF 257



>XP_006712078
          Length = 389

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KRMAVLIF 8
           K+MA+LIF
Sbjct: 259 KKMAILIF 266



>XP_005274043
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KRMAVLIFI 9
           KR+ +L+F+
Sbjct: 348 KRICILVFL 356



>XP_006718611
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KRMAVLIFI 9
           KR+ +L+F+
Sbjct: 240 KRICILVFL 248



>XP_006718610
          Length = 489

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KRMAVLIFI 9
           KR+ +L+F+
Sbjct: 296 KRICILVFL 304



>NP_001034841
          Length = 541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KRMAVLIFI 9
           KR+ +L+F+
Sbjct: 348 KRICILVFL 356



>NP_068587
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KRMAVLIFI 9
           KRM  L+FI
Sbjct: 124 KRMGGLLFI 132



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.361    0.163    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 268,343
Number of extensions: 337
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.2 bits)
S2: 28 (15.4 bits)