Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001161328                                                           17    2528
NP_060653                                                              17    2528
XP_005272668                                                           17    2528
XP_005272667                                                           17    2528
XP_006724596                                                           17    2528
XP_005253633                                                           17    2528
XP_005253632                                                           17    2528
XP_006719537                                                           17    2528
XP_006719536                                                           17    2528
XP_005253630                                                           17    2528
XP_005253629                                                           17    2528
NP_006725                                                              17    3301
XP_005267014                                                           17    3301
NP_003631                                                              17    4312
XP_005252342                                                           17    4312
NP_653081                                                              16    5631
NP_055650                                                              16    5631
NP_001265578                                                           16    5631
XP_005260682                                                           16    5631
NP_000069                                                              16    7355
NP_001273014                                                           16    7355
NP_008937                                                              16    7355
NP_001182682                                                           16    7355
NP_001182681                                                           16    7355
NP_003569                                                              16    7355
XP_005263589                                                           16    7355
NP_004374                                                              15    9605
NP_001120662                                                           15    9605
NP_065077                                                              15    9605
NP_001180217                                                           15    9605
NP_006428                                                              15    9605
NP_001253989                                                           15    9605
NP_001253990                                                           15    9605
NP_001253988                                                           15    9605
NP_055563                                                              15    9605
NP_005301                                                              15    9605
NP_001269340                                                           15    9605
NP_002703                                                              15    9605
NP_001269338                                                           15    9605
NP_001269343                                                           15    9605
NP_001269339                                                           15    9605
NP_001269342                                                           15    9605
NP_647611                                                              15    9605
NP_647612                                                              15    9605
NP_001269341                                                           15    9605
NP_064710                                                              15    9605
NP_002369                                                              15    9605
NP_061927                                                              15    9605
NP_001229371                                                           15    9605
NP_001010979                                                           15    9605
NP_001025173                                                           15    9605
NP_001229372                                                           15    9605
XP_005255493                                                           15    9605
XP_006721128                                                           15    9605
XP_005255492                                                           15    9605
XP_005254222                                                           15    9605
XP_006717968                                                           15    9605
XP_006717967                                                           15    9605
XP_006717966                                                           15    9605
XP_006717965                                                           15    9605
XP_006716813                                                           15    9605
XP_005251482                                                           15    9605
XP_005251483                                                           15    9605
NP_776272                                                              15    9605
>NP_001161328
          Length = 1393

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1227 KKKLFLV 1233
>NP_060653
          Length = 1392

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1226 KKKLFLV 1232
>XP_005272668
          Length = 504

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KKKLFLVRE 9
          K KLFL+R+
Sbjct: 76 KAKLFLIRD 84
>XP_005272667
          Length = 539

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KKKLFLVRE 9
          K KLFL+R+
Sbjct: 76 KAKLFLIRD 84
>XP_006724596
          Length = 562

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KKKLFLVRE 9
          K KLFL+R+
Sbjct: 76 KAKLFLIRD 84
>XP_005253633
          Length = 1392

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1226 KKKLFLV 1232
>XP_005253632
          Length = 1392

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1226 KKKLFLV 1232
>XP_006719537
          Length = 1393

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1227 KKKLFLV 1233
>XP_006719536
          Length = 1393

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1227 KKKLFLV 1233
>XP_005253630
          Length = 1393

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1227 KKKLFLV 1233
>XP_005253629
          Length = 1393

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    KKKLFLV 7
            KKKLFLV
Sbjct: 1227 KKKLFLV 1233
>NP_006725
          Length = 2446

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KKKLFLVRE 9
            +KK FLVR+
Sbjct: 1075 RKKCFLVRQ 1083
>XP_005267014
          Length = 2446

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KKKLFLVRE 9
            +KK FLVR+
Sbjct: 1075 RKKCFLVRQ 1083
>NP_003631
          Length = 1332

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KKKLFLVRE 9
            KK+L +VRE
Sbjct: 1135 KKRLLVVRE 1143
>XP_005252342
          Length = 1218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KKKLFLVRE 9
            KK+L +VRE
Sbjct: 1021 KKRLLVVRE 1029
>NP_653081
          Length = 480

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKKLFLV 7
           KKKLF++
Sbjct: 292 KKKLFMI 298
>NP_055650
          Length = 468

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKKLFLV 7
           KKKLF++
Sbjct: 280 KKKLFMI 286
>NP_001265578
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKKLFLV 7
           KKKLF++
Sbjct: 200 KKKLFMI 206
>XP_005260682
          Length = 267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  KKKLFLV 7
          KKKLF++
Sbjct: 79 KKKLFMI 85
>NP_000069
          Length = 493

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KKKLFL 6
           KKKLFL
Sbjct: 394 KKKLFL 399
>NP_001273014
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KKKLFL 6
           KKKLFL
Sbjct: 334 KKKLFL 339
>NP_008937
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KKLFLVR 8
           KKLFLV+
Sbjct: 172 KKLFLVQ 178
>NP_001182682
          Length = 239

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KKKLFL 6
           KKKLFL
Sbjct: 189 KKKLFL 194
>NP_001182681
          Length = 238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KKKLFL 6
           KKKLFL
Sbjct: 188 KKKLFL 193
>NP_003569
          Length = 522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   KKKLFLVRE 9
           K+K+F++R+
Sbjct: 100 KQKVFIIRK 108
>XP_005263589
          Length = 238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KKKLFL 6
           KKKLFL
Sbjct: 188 KKKLFL 193
>NP_004374
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 103 LFLVRE 108
>NP_001120662
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 103 LFLVRE 108
>NP_065077
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KKKLFLVR 8
          KKKL L+R
Sbjct: 31 KKKLKLIR 38
>NP_001180217
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KKKLFLVR 8
          KKKL L+R
Sbjct: 31 KKKLKLIR 38
>NP_006428
          Length = 1724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKKLFLVRE 9
           KK+ FL+RE
Sbjct: 223 KKQGFLLRE 231
>NP_001253989
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 137 LFLVRE 142
>NP_001253990
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 142 LFLVRE 147
>NP_001253988
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 150 LFLVRE 155
>NP_055563
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 162 LFLVRE 167
>NP_005301
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 454 LFLVRE 459
>NP_001269340
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 124 KKLFLV 129
>NP_002703
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 167 KKLFLV 172
>NP_001269338
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 124 KKLFLV 129
>NP_001269343
          Length = 221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 108 KKLFLV 113
>NP_001269339
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 167 KKLFLV 172
>NP_001269342
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 108 KKLFLV 113
>NP_647611
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 102 LFLVRE 107
>NP_647612
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 143 LFLVRE 148
>NP_001269341
          Length = 344

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KKLFLV 7
           KKLFLV
Sbjct: 151 KKLFLV 156
>NP_064710
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 884 LFLVRE 889
>NP_002369
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 144 LFLVRE 149
>NP_061927
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKKLFLVR 8
           KKK+FL R
Sbjct: 483 KKKVFLWR 490
>NP_001229371
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 477 LFLVRE 482
>NP_001010979
          Length = 101

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  KKKLFLVRE 9
          KK+L +VR+
Sbjct: 63 KKQLLMVRQ 71
>NP_001025173
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 454 LFLVRE 459
>NP_001229372
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 454 LFLVRE 459
>XP_005255493
          Length = 959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 791 LFLVRE 796
>XP_006721128
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 817 LFLVRE 822
>XP_005255492
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 858 LFLVRE 863
>XP_005254222
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFLVRE 9
           LFLVRE
Sbjct: 103 LFLVRE 108
>XP_006717968
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKKLFLVR 8
           KKK+FL R
Sbjct: 483 KKKVFLWR 490
>XP_006717967
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKKLFLVR 8
           KKK+FL R
Sbjct: 483 KKKVFLWR 490
>XP_006717966
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKKLFLVR 8
           KKK+FL R
Sbjct: 483 KKKVFLWR 490
>XP_006717965
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKKLFLVR 8
           KKK+FL R
Sbjct: 489 KKKVFLWR 496
>XP_006716813
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KKKLFLVR 8
          KKKL L+R
Sbjct: 31 KKKLKLIR 38
>XP_005251482
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KKKLFLVR 8
          KKKL L+R
Sbjct: 31 KKKLKLIR 38
>XP_005251483
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KKKLFLVR 8
          KKKL L+R
Sbjct: 31 KKKLKLIR 38
>NP_776272
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKKLFLVRE 9
           KKK  L+RE
Sbjct: 268 KKKRLLLRE 276
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.147    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 528,786
Number of extensions: 949
Number of successful extensions: 64
Number of sequences better than 10000.0: 64
Number of HSP's gapped: 64
Number of HSP's successfully gapped: 64
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package