Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     299
NP_000242                                                              20     299
XP_005264389                                                           20     299
NP_937878                                                              17    4312
NP_937875                                                              17    4312
NP_002924                                                              17    4312
NP_937877                                                              17    4312
NP_056076                                                              16    5631
NP_000013                                                              16    7355
NP_001017975                                                           16    7355
NP_061883                                                              16    7355
XP_005277849                                                           16    7355
XP_005260293                                                           16    7355
XP_006723742                                                           16    7355
XP_005257521                                                           16    7355
XP_006710458                                                           16    7355
NP_001136357                                                           15    9605
NP_003744                                                              15    9605
NP_055363                                                              15    9605
NP_003649                                                              15    9605

>NP_001245210
          Length = 868

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KKFQRQTAN 9
           KKFQRQ AN
Sbjct: 326 KKFQRQAAN 334



>NP_000242
          Length = 934

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KKFQRQTAN 9
           KKFQRQ AN
Sbjct: 392 KKFQRQAAN 400



>XP_005264389
          Length = 921

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KKFQRQTAN 9
           KKFQRQ AN
Sbjct: 392 KKFQRQAAN 400



>NP_937878
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KKFQRQ 6
          KKFQRQ
Sbjct: 34 KKFQRQ 39



>NP_937875
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KKFQRQ 6
          KKFQRQ
Sbjct: 34 KKFQRQ 39



>NP_002924
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KKFQRQ 6
          KKFQRQ
Sbjct: 34 KKFQRQ 39



>NP_937877
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KKFQRQ 6
          KKFQRQ
Sbjct: 34 KKFQRQ 39



>NP_056076
          Length = 1498

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FQRQTAN 9
           +QRQT+N
Sbjct: 512 YQRQTSN 518



>NP_000013
          Length = 363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKFQRQT 7
           KK+Q+QT
Sbjct: 170 KKYQQQT 176



>NP_001017975
          Length = 1435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KFQRQTAN 9
            KFQR+ +N
Sbjct: 1317 KFQREMSN 1324



>NP_061883
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKFQRQTA 8
           ++F+RQTA
Sbjct: 259 EQFERQTA 266



>XP_005277849
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKFQRQTA 8
           ++F+RQTA
Sbjct: 259 EQFERQTA 266



>XP_005260293
          Length = 339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKFQRQT 7
           KK+Q+QT
Sbjct: 170 KKYQQQT 176



>XP_006723742
          Length = 354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKFQRQT 7
           KK+Q+QT
Sbjct: 170 KKYQQQT 176



>XP_005257521
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKFQRQTA 8
           ++F+RQTA
Sbjct: 259 EQFERQTA 266



>XP_006710458
          Length = 846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KFQRQTAN 9
           KFQR+ +N
Sbjct: 728 KFQREMSN 735



>NP_001136357
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KFQRQTAN 9
           KF  +TAN
Sbjct: 148 KFDEETAN 155



>NP_003744
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KKFQRQ 6
           KKFQ+Q
Sbjct: 138 KKFQKQ 143



>NP_055363
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KFQRQTAN 9
           KF  +TAN
Sbjct: 148 KFDEETAN 155



>NP_003649
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KKFQRQ 6
           KKFQ+Q
Sbjct: 150 KKFQKQ 155



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.117    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 409,007
Number of extensions: 488
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)