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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055876                                                              19     869
XP_006716804                                                           19     869
NP_055626                                                              18    1935
XP_006717405                                                           18    1935
XP_006717404                                                           18    1935
XP_006717403                                                           18    1935
XP_005263469                                                           18    1935
XP_005263468                                                           18    1935
NP_001128668                                                           17    4312
XP_006717666                                                           17    4312
XP_005269535                                                           17    4312
XP_006717665                                                           17    4312
XP_005269533                                                           17    4312
XP_005269534                                                           17    4312
NP_001159579                                                           15    9605
NP_005509                                                              15    9605
NP_201586                                                              15    9605

>NP_055876
          Length = 1056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KARFSTAS 9
            KARFSTAS
Sbjct: 995  KARFSTAS 1002



>XP_006716804
          Length = 1089

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    KARFSTAS 9
            KARFSTAS
Sbjct: 1028 KARFSTAS 1035



>NP_055626
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KKARFSTA 8
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_006717405
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KKARFSTA 8
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_006717404
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KKARFSTA 8
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_006717403
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KKARFSTA 8
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_005263469
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KKARFSTA 8
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>XP_005263468
          Length = 1209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KKARFSTA 8
           KK RFSTA
Sbjct: 720 KKVRFSTA 727



>NP_001128668
          Length = 1435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KARFSTAS 9
           KA+FST+S
Sbjct: 725 KAKFSTSS 732



>XP_006717666
          Length = 1289

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KARFSTAS 9
           KA+FST+S
Sbjct: 579 KAKFSTSS 586



>XP_005269535
          Length = 1435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KARFSTAS 9
           KA+FST+S
Sbjct: 725 KAKFSTSS 732



>XP_006717665
          Length = 1435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KARFSTAS 9
           KA+FST+S
Sbjct: 725 KAKFSTSS 732



>XP_005269533
          Length = 1435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KARFSTAS 9
           KA+FST+S
Sbjct: 725 KAKFSTSS 732



>XP_005269534
          Length = 1435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KARFSTAS 9
           KA+FST+S
Sbjct: 725 KAKFSTSS 732



>NP_001159579
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  RFSTAS 9
          RFSTAS
Sbjct: 35 RFSTAS 40



>NP_005509
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  RFSTAS 9
          RFSTAS
Sbjct: 35 RFSTAS 40



>NP_201586
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  RFSTAS 9
          RFSTAS
Sbjct: 21 RFSTAS 26



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.107    0.255 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 288,112
Number of extensions: 252
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)