Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              19     665
NP_001005489                                                           17    4312
NP_077306                                                              16    5631
XP_005276791                                                           16    5631
XP_005244909                                                           16    5631
NP_001010860                                                           16    7355
NP_001182180                                                           15    9605
NP_778239                                                              15    9605
NP_001182181                                                           15    9605
NP_001128527                                                           15    9605
NP_001244957                                                           15    9605
NP_001055                                                              15    9605
NP_004059                                                              15    9605
NP_001020376                                                           15    9605
NP_001182183                                                           15    9605
NP_001182178                                                           15    9605
NP_778243                                                              15    9605
NP_001182179                                                           15    9605
NP_001182177                                                           15    9605
XP_006716855                                                           15    9605
XP_006716854                                                           15    9605
XP_006713382                                                           15    9605
NP_001088                                                              15    9605
>NP_115803
          Length = 1156

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KIVIKSKYS 9
           KIVIKS+YS
Sbjct: 361 KIVIKSEYS 369
>NP_001005489
          Length = 314

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KIVIKSKYS 9
           K+V K+KYS
Sbjct: 301 KVVEKAKYS 309
>NP_077306
          Length = 730

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IVIKSKYS 9
           +V+KSK+S
Sbjct: 396 VVLKSKFS 403
>XP_005276791
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IVIKSKYS 9
           +V+KSK+S
Sbjct: 156 VVLKSKFS 163
>XP_005244909
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IVIKSKYS 9
           +V+KSK+S
Sbjct: 156 VVLKSKFS 163
>NP_001010860
          Length = 674

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   IKSKYS 9
           IKSKYS
Sbjct: 428 IKSKYS 433
>NP_001182180
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 189 VVIKDKY 195
>NP_778239
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 203 VVIKDKY 209
>NP_001182181
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 131 VVIKDKY 137
>NP_001128527
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KIVIKSK 7
           K+V+KSK
Sbjct: 493 KVVLKSK 499
>NP_001244957
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KIVIKSK 7
           K+V+KSK
Sbjct: 501 KVVLKSK 507
>NP_001055
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KIVIKSK 7
           K+V+KSK
Sbjct: 493 KVVLKSK 499
>NP_004059
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIVIKSKY 8
           K+VIKS +
Sbjct: 304 KVVIKSNF 311
>NP_001020376
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIVIKSKY 8
           K+VIKS +
Sbjct: 302 KVVIKSNF 309
>NP_001182183
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 135 VVIKDKY 141
>NP_001182178
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 189 VVIKDKY 195
>NP_778243
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 189 VVIKDKY 195
>NP_001182179
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 149 VVIKDKY 155
>NP_001182177
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 203 VVIKDKY 209
>XP_006716855
          Length = 254

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 101 VVIKDKY 107
>XP_006716854
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IVIKSKY 8
           +VIK KY
Sbjct: 189 VVIKDKY 195
>XP_006713382
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KIVIKSK 7
           K+V+KSK
Sbjct: 493 KVVLKSK 499
>NP_001088
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KIVIKSKYS 9
           KI+I  KY+
Sbjct: 127 KIIIHEKYN 135
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.128    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 401,890
Number of extensions: 481
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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