Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_644807                                                              18    1935
NP_112598                                                              17    2528
XP_005250685                                                           17    4312
XP_005250684                                                           17    4312
XP_005250683                                                           17    4312
XP_005250682                                                           17    4312
NP_874358                                                              16    5631
NP_005088                                                              16    7355
NP_060606                                                              16    7355
NP_056340                                                              16    7355
XP_005270329                                                           16    7355
XP_006715518                                                           16    7355
XP_006715517                                                           16    7355
XP_006715516                                                           16    7355
NP_000245                                                              15    9605
NP_001035351                                                           15    9605
NP_001035350                                                           15    9605
NP_068566                                                              15    9605
NP_001138899                                                           15    9605
NP_001096045                                                           15    9605
NP_443102                                                              15    9605
NP_001838                                                              15    9605
NP_543138                                                              15    9605
NP_079382                                                              15    9605
NP_001274688                                                           15    9605
NP_378667                                                              15    9605
NP_001274687                                                           15    9605
NP_001274689                                                           15    9605
XP_006724681                                                           15    9605
XP_006724680                                                           15    9605
XP_005262843                                                           15    9605
XP_005262842                                                           15    9605
XP_005273202                                                           15    9605
XP_006711833                                                           15    9605
XP_006711832                                                           15    9605
XP_005273200                                                           15    9605
XP_005273198                                                           15    9605

>NP_644807
          Length = 548

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KISFRGMKS 9
           K +FRG+KS
Sbjct: 129 KFTFRGLKS 137



>NP_112598
          Length = 5088

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KISFRGMK 8
            K+SFRG++
Sbjct: 2707 KVSFRGLR 2714




 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KISFRGMK 8
            K+SFRG++
Sbjct: 3238 KVSFRGLR 3245




 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KISFRGMK 8
            K+SFRG++
Sbjct: 3772 KVSFRGLR 3779




 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KISFRGMK 8
            K+SFRG++
Sbjct: 4306 KVSFRGLR 4313




 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KISFRGMK 8
            K+SFRG++
Sbjct: 4839 KVSFRGLR 4846



>XP_005250685
          Length = 193

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KISFRGMKS 9
           +I+FRGM++
Sbjct: 178 RITFRGMEN 186



>XP_005250684
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KISFRGMKS 9
           +I+FRGM++
Sbjct: 189 RITFRGMEN 197



>XP_005250683
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KISFRGMKS 9
           +I+FRGM++
Sbjct: 189 RITFRGMEN 197



>XP_005250682
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KISFRGMKS 9
           +I+FRGM++
Sbjct: 189 RITFRGMEN 197



>NP_874358
          Length = 631

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KISFRGMKS 9
           K S++G+KS
Sbjct: 318 KYSYKGLKS 326



>NP_005088
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           +FRG+KS
Sbjct: 134 TFRGLKS 140



>NP_060606
          Length = 3477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    SFRGMKS 9
            +FRGMK+
Sbjct: 2394 AFRGMKT 2400




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SFRGMK 8
            +FRGMK
Sbjct: 2748 AFRGMK 2753



>NP_056340
          Length = 1353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    KISFRGMKS 9
            K+SF+G+++
Sbjct: 1226 KVSFKGIEN 1234



>XP_005270329
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           +FRG+KS
Sbjct: 134 TFRGLKS 140



>XP_006715518
          Length = 1338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    KISFRGMKS 9
            K+SF+G+++
Sbjct: 1211 KVSFKGIEN 1219



>XP_006715517
          Length = 1379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    KISFRGMKS 9
            K+SF+G+++
Sbjct: 1211 KVSFKGIEN 1219



>XP_006715516
          Length = 1394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1    KISFRGMKS 9
            K+SF+G+++
Sbjct: 1226 KVSFKGIEN 1234



>NP_000245
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           SFRGM++
Sbjct: 583 SFRGMEA 589



>NP_001035351
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SFRG+K
Sbjct: 364 SFRGLK 369



>NP_001035350
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SFRG+K
Sbjct: 347 SFRGLK 352



>NP_068566
          Length = 790

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  KISFRG 6
          K+SFRG
Sbjct: 48 KLSFRG 53



>NP_001138899
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMKS 9
           F+GMKS
Sbjct: 289 FKGMKS 294



>NP_001096045
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMKS 9
           F+GMKS
Sbjct: 289 FKGMKS 294



>NP_443102
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FRGMKS 9
           F+GMKS
Sbjct: 289 FKGMKS 294



>NP_001838
          Length = 1691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 166 SFKGMK 171



>NP_543138
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   ISFRGMK 8
           ++FRG+K
Sbjct: 199 VAFRGLK 205



>NP_079382
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   ISFRGMK 8
           ++FRG+K
Sbjct: 199 VAFRGLK 205



>NP_001274688
          Length = 1666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 165 SFKGMK 170



>NP_378667
          Length = 1690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 165 SFKGMK 170



>NP_001274687
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 165 SFKGMK 170



>NP_001274689
          Length = 1633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 165 SFKGMK 170



>XP_006724681
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 166 SFKGMK 171



>XP_006724680
          Length = 1708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SF+GMK
Sbjct: 166 SFKGMK 171



>XP_005262843
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SFRG+K
Sbjct: 347 SFRGLK 352



>XP_005262842
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SFRGMK 8
           SFRG+K
Sbjct: 364 SFRGLK 369



>XP_005273202
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SFRGMKS 9
          SFRGM++
Sbjct: 37 SFRGMEA 43



>XP_006711833
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           SFRGM++
Sbjct: 271 SFRGMEA 277



>XP_006711832
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           SFRGM++
Sbjct: 583 SFRGMEA 589



>XP_005273200
          Length = 1214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           SFRGM++
Sbjct: 583 SFRGMEA 589



>XP_005273198
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SFRGMKS 9
           SFRGM++
Sbjct: 582 SFRGMEA 588



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.134    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 394,102
Number of extensions: 511
Number of successful extensions: 42
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)