Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     665
NP_000240                                                              19     665
NP_001161089                                                           19     665
XP_005265218                                                           19     665
NP_003137                                                              17    4312
XP_005274251                                                           17    4312
NP_002212                                                              16    5631
NP_056153                                                              16    5631
NP_001265577                                                           16    5631
NP_001265576                                                           16    5631
NP_663627                                                              16    5631
NP_005332                                                              16    5631
XP_006723795                                                           16    5631
XP_006723794                                                           16    5631
XP_006723793                                                           16    5631
XP_006723792                                                           16    5631
XP_006723791                                                           16    5631
XP_006723790                                                           16    5631
XP_005273177                                                           16    5631
XP_006710655                                                           16    5631
NP_997217                                                              16    5631
NP_697019                                                              16    5631
NP_001139811                                                           16    7355
NP_937878                                                              16    7355
NP_937875                                                              16    7355
NP_002924                                                              16    7355
NP_937877                                                              16    7355

>NP_001245200
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KCAYRAS 7
           KCAYRAS
Sbjct: 123 KCAYRAS 129



>NP_000240
          Length = 756

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KCAYRAS 7
           KCAYRAS
Sbjct: 123 KCAYRAS 129



>NP_001161089
          Length = 658

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  KCAYRAS 7
          KCAYRAS
Sbjct: 25 KCAYRAS 31



>XP_005265218
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KCAYRAS 7
           KCAYRAS
Sbjct: 123 KCAYRAS 129



>NP_003137
          Length = 709

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   CAYRASS 8
           C+Y+ASS
Sbjct: 343 CSYKASS 349



>XP_005274251
          Length = 712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   CAYRASS 8
           C+Y+ASS
Sbjct: 343 CSYKASS 349



>NP_002212
          Length = 946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CAYRASSQ 9
           C +R+SSQ
Sbjct: 178 CPFRSSSQ 185



>NP_056153
          Length = 1541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 151 SYRASSQ 157



>NP_001265577
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KCAYRASSQ 9
           +C +RASS+
Sbjct: 440 ECGHRASSR 448



>NP_001265576
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KCAYRASSQ 9
           +C +RASS+
Sbjct: 440 ECGHRASSR 448



>NP_663627
          Length = 123

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  KCAYRA 6
          KC YRA
Sbjct: 60 KCCYRA 65



>NP_005332
          Length = 688

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KCAYRASSQ 9
           +C +RASS+
Sbjct: 440 ECGHRASSR 448



>XP_006723795
          Length = 1480

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 141 SYRASSQ 147



>XP_006723794
          Length = 1490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 151 SYRASSQ 157



>XP_006723793
          Length = 1513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 123 SYRASSQ 129



>XP_006723792
          Length = 1513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 123 SYRASSQ 129



>XP_006723791
          Length = 1531

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 141 SYRASSQ 147



>XP_006723790
          Length = 1540

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AYRASSQ 9
           +YRASSQ
Sbjct: 150 SYRASSQ 156



>XP_005273177
          Length = 644

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CAYRASSQ 9
           C +R+SSQ
Sbjct: 178 CPFRSSSQ 185



>XP_006710655
          Length = 346

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KCAYRASSQ 9
           +C +RASS+
Sbjct: 98  ECGHRASSR 106



>NP_997217
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   KCAYR 5
           KCAYR
Sbjct: 243 KCAYR 247



>NP_697019
          Length = 67

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  KCAYRASSQ 9
          KC YR S +
Sbjct: 31 KCRYRCSKK 39



>NP_001139811
          Length = 285

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  CAYRAS 7
          CA+RAS
Sbjct: 49 CAFRAS 54



>NP_937878
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CAYRAS 7
           CAYR S
Sbjct: 123 CAYRTS 128



>NP_937875
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CAYRAS 7
           CAYR S
Sbjct: 123 CAYRTS 128



>NP_002924
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CAYRAS 7
           CAYR S
Sbjct: 123 CAYRTS 128



>NP_937877
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CAYRAS 7
           CAYR S
Sbjct: 123 CAYRTS 128



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.112    0.334 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 476,868
Number of extensions: 434
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)