Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001238830                                                           17    3301
NP_001035370                                                           17    3301
NP_000360                                                              17    3301
NP_001157849                                                           17    3301
XP_005268094                                                           17    3301
NP_004224                                                              17    3301
NP_001129047                                                           16    5631
NP_060051                                                              16    5631
NP_061722                                                              16    7355
NP_115655                                                              16    7355
NP_001248770                                                           16    7355
XP_005261433                                                           16    7355
XP_005255642                                                           16    7355
XP_005247886                                                           16    7355
XP_006713849                                                           16    7355
NP_001098718                                                           16    7355
NP_079056                                                              16    7355
NP_114089                                                              16    7355
NP_004358                                                              15    9605
NP_113597                                                              15    9605
NP_001231875                                                           15    9605
NP_076406                                                              15    9605
NP_001004479                                                           15    9605
>NP_001238830
          Length = 146

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IVFVLTLII 9
           ++F LTLII
Sbjct: 93  VIFYLTLII 101
>NP_001035370
          Length = 204

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IVFVLTLII 9
           ++F LTLII
Sbjct: 152 VIFYLTLII 160
>NP_000360
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IVFVLTLII 9
           IVFVLTL I
Sbjct: 583 IVFVLTLNI 591
>NP_001157849
          Length = 289

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IVFVLTLII 9
          +VF LTLI+
Sbjct: 14 VVFYLTLIV 22
>XP_005268094
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IVFVLTLII 9
           IVFVLTL I
Sbjct: 583 IVFVLTLNI 591
>NP_004224
          Length = 205

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IVFVLTLII 9
           ++F LTLII
Sbjct: 152 VIFYLTLII 160
>NP_001129047
          Length = 91

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IVFVLTLII 9
          +VFV TL+I
Sbjct: 10 LVFVATLLI 18
>NP_060051
          Length = 479

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   IVFVLTLII 9
           +VFV+T++I
Sbjct: 466 MVFVMTILI 474
>NP_061722
          Length = 1007

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VFVLTLII 9
           +F+LT+II
Sbjct: 718 IFLLTIII 725
>NP_115655
          Length = 1196

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VFVLTLII 9
            +FVL LII
Sbjct: 1071 IFVLVLII 1078
>NP_001248770
          Length = 1006

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FVLTLII 9
           F+LTLI+
Sbjct: 909 FILTLIV 915
>XP_005261433
          Length = 289

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IVFVLTLI 8
          +VF+LTL+
Sbjct: 7  VVFLLTLL 14
>XP_005255642
          Length = 647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FVLTLII 9
           F+LTLI+
Sbjct: 550 FILTLIV 556
>XP_005247886
          Length = 1127

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VFVLTLII 9
            +FVL LII
Sbjct: 1071 IFVLVLII 1078
>XP_006713849
          Length = 1147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VFVLTLII 9
            +FVL LII
Sbjct: 1022 IFVLVLII 1029
>NP_001098718
          Length = 1006

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FVLTLII 9
           F+LTLI+
Sbjct: 909 FILTLIV 915
>NP_079056
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FVLTLII 9
           F+LTLI+
Sbjct: 663 FILTLIV 669
>NP_114089
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VFVLTLII 9
           +F+LT+II
Sbjct: 718 IFLLTIII 725
>NP_004358
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  IVFVLTL 7
          I+FVLTL
Sbjct: 93 ILFVLTL 99
>NP_113597
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  IVFVLTL 7
          I+FVLTL
Sbjct: 93 ILFVLTL 99
>NP_001231875
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VFVLTLII 9
           VF+LTL++
Sbjct: 412 VFLLTLLL 419
>NP_076406
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IVFVLTLII 9
           I+F+L LI+
Sbjct: 235 IIFLLLLIV 243
>NP_001004479
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  IVFVLTLI 8
          +++VLTL+
Sbjct: 43 VIYVLTLL 50
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.362    0.179    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 315,132
Number of extensions: 551
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.1 bits)
S2: 28 (15.4 bits)

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