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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245202                                                           18    1135
NP_001245200                                                           18    1135
NP_001161091                                                           18    1135
NP_000240                                                              18    1135
NP_001161090                                                           18    1135
NP_001161089                                                           18    1135
NP_001245203                                                           18    1135
XP_005265221                                                           18    1135
XP_005265220                                                           18    1135
NP_000043                                                              17    4312
NP_001269153                                                           17    4312
NP_653319                                                              16    5631
XP_005248295                                                           16    5631
XP_006713578                                                           16    5631
NP_009208                                                              16    5631
NP_006050                                                              16    7355
XP_005248148                                                           16    7355
XP_005248147                                                           16    7355
XP_005248146                                                           16    7355
XP_005248145                                                           16    7355
XP_005248142                                                           16    7355
XP_005248141                                                           16    7355
XP_005248140                                                           16    7355
XP_005248139                                                           16    7355
NP_037397                                                              15    9605
NP_004121                                                              15    9605
NP_001171649                                                           15    9605
NP_060921                                                              15    9605
NP_001171650                                                           15    9605
NP_037498                                                              15    9605

>NP_001245202
          Length = 515

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  SLRKAIET 9
          SLRKAIET
Sbjct: 30 SLRKAIET 37



>NP_001245200
          Length = 687

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKAIET 9
           SLRKAIET
Sbjct: 271 SLRKAIET 278



>NP_001161091
          Length = 515

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  SLRKAIET 9
          SLRKAIET
Sbjct: 30 SLRKAIET 37



>NP_000240
          Length = 756

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKAIET 9
           SLRKAIET
Sbjct: 271 SLRKAIET 278



>NP_001161090
          Length = 515

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  SLRKAIET 9
          SLRKAIET
Sbjct: 30 SLRKAIET 37



>NP_001161089
          Length = 658

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SLRKAIET 9
           SLRKAIET
Sbjct: 173 SLRKAIET 180



>NP_001245203
          Length = 515

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  SLRKAIET 9
          SLRKAIET
Sbjct: 30 SLRKAIET 37



>XP_005265221
          Length = 515

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  SLRKAIET 9
          SLRKAIET
Sbjct: 30 SLRKAIET 37



>XP_005265220
          Length = 515

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  SLRKAIET 9
          SLRKAIET
Sbjct: 30 SLRKAIET 37



>NP_000043
          Length = 1500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   SLRKAIE 8
           SLRKAIE
Sbjct: 330 SLRKAIE 336



>NP_001269153
          Length = 1422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   SLRKAIE 8
           SLRKAIE
Sbjct: 330 SLRKAIE 336



>NP_653319
          Length = 855

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISLRKAIET 9
           I LRK +ET
Sbjct: 388 IQLRKEVET 396



>XP_005248295
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LRKAIET 9
           LRKA+ET
Sbjct: 395 LRKALET 401



>XP_006713578
          Length = 855

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISLRKAIET 9
           I LRK +ET
Sbjct: 388 IQLRKEVET 396



>NP_009208
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LRKAIET 9
           LRKA+ET
Sbjct: 395 LRKALET 401



>NP_006050
          Length = 1575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    ISLRKAIET 9
            ISL K IET
Sbjct: 1476 ISLEKDIET 1484



>XP_005248148
          Length = 3028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 351 SIRKQIET 358



>XP_005248147
          Length = 3028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 351 SIRKQIET 358



>XP_005248146
          Length = 3073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 443 SIRKQIET 450



>XP_005248145
          Length = 3079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 402 SIRKQIET 409



>XP_005248142
          Length = 3114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 443 SIRKQIET 450



>XP_005248141
          Length = 3119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 443 SIRKQIET 450



>XP_005248140
          Length = 3119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 443 SIRKQIET 450



>XP_005248139
          Length = 3120

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLRKAIET 9
           S+RK IET
Sbjct: 443 SIRKQIET 450



>NP_037397
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LRKAIET 9
           LRK++ET
Sbjct: 591 LRKSVET 597



>NP_004121
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SLRKAIET 9
          S+RK +ET
Sbjct: 46 SMRKVLET 53



>NP_001171649
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SLRKAIET 9
          S+RK +ET
Sbjct: 46 SMRKVLET 53



>NP_060921
          Length = 1338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISLRKAIET 9
           +SL+K +ET
Sbjct: 555 VSLQKKLET 563



>NP_001171650
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SLRKAIET 9
          S+RK +ET
Sbjct: 46 SMRKVLET 53



>NP_037498
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ISLRKAIET 9
           +SL+ A+ET
Sbjct: 376 VSLKAALET 384



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.130    0.297 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,444
Number of extensions: 703
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)