Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              17    3301
XP_005271621                                                           17    3301
XP_006718907                                                           17    3301
XP_006718906                                                           17    3301
XP_005271619                                                           17    3301
XP_005271618                                                           17    3301
NP_055068                                                              16    5631
NP_001156750                                                           16    5631
NP_001156751                                                           16    5631
NP_001838                                                              16    5631
NP_001274688                                                           16    5631
NP_378667                                                              16    5631
NP_001274687                                                           16    5631
NP_001274689                                                           16    5631
XP_006724681                                                           16    5631
XP_006724680                                                           16    5631
XP_006718871                                                           16    5631
XP_005250685                                                           16    5631
XP_005250684                                                           16    5631
XP_005250683                                                           16    5631
XP_005250682                                                           16    5631
NP_835228                                                              16    5631
NP_005088                                                              16    7355
NP_060606                                                              16    7355
NP_004058                                                              16    7355
NP_001035025                                                           16    7355
NP_644807                                                              16    7355
NP_543138                                                              16    7355
NP_079382                                                              16    7355
NP_112598                                                              16    7355
XP_005270329                                                           16    7355
XP_005249659                                                           16    7355
XP_005249656                                                           16    7355
XP_005273067                                                           16    7355
NP_006606                                                              16    7355
NP_057536                                                              16    7355
NP_000245                                                              15    9605
NP_001035351                                                           15    9605
NP_001035350                                                           15    9605
NP_001846                                                              15    9605
NP_942014                                                              15    9605
NP_001138899                                                           15    9605
NP_001096045                                                           15    9605
NP_443102                                                              15    9605
XP_005266846                                                           15    9605
XP_005266845                                                           15    9605
XP_005266844                                                           15    9605
XP_005262843                                                           15    9605
XP_005262842                                                           15    9605
XP_005273202                                                           15    9605
XP_006711833                                                           15    9605
XP_006711832                                                           15    9605
XP_005273200                                                           15    9605
XP_005273198                                                           15    9605
>NP_000042
          Length = 3056

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           I+FRG+ SE
Sbjct: 490 ITFRGISSE 498
>XP_005271621
          Length = 2708

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           I+FRG+ SE
Sbjct: 142 ITFRGISSE 150
>XP_006718907
          Length = 3050

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           I+FRG+ SE
Sbjct: 490 ITFRGISSE 498
>XP_006718906
          Length = 3056

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           I+FRG+ SE
Sbjct: 490 ITFRGISSE 498
>XP_005271619
          Length = 3056

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           I+FRG+ SE
Sbjct: 490 ITFRGISSE 498
>XP_005271618
          Length = 3056

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           I+FRG+ SE
Sbjct: 490 ITFRGISSE 498
>NP_055068
          Length = 2725

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGMKSE 9
           RGMKSE
Sbjct: 126 RGMKSE 131
>NP_001156750
          Length = 2732

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGMKSE 9
           RGMKSE
Sbjct: 126 RGMKSE 131
>NP_001156751
          Length = 2731

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RGMKSE 9
           RGMKSE
Sbjct: 126 RGMKSE 131
>NP_001838
          Length = 1691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 166 SFKGMKGD 173
>NP_001274688
          Length = 1666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 165 SFKGMKGD 172
>NP_378667
          Length = 1690

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 165 SFKGMKGD 172
>NP_001274687
          Length = 1707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 165 SFKGMKGD 172
>NP_001274689
          Length = 1633

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 165 SFKGMKGD 172
>XP_006724681
          Length = 1249

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 166 SFKGMKGD 173
>XP_006724680
          Length = 1708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           SF+GMK +
Sbjct: 166 SFKGMKGD 173
>XP_006718871
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           +FRG+K E
Sbjct: 220 AFRGLKKE 227
>XP_005250685
          Length = 193

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISFRGMKSE 9
           I+FRGM+++
Sbjct: 179 ITFRGMENK 187
>XP_005250684
          Length = 204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISFRGMKSE 9
           I+FRGM+++
Sbjct: 190 ITFRGMENK 198
>XP_005250683
          Length = 204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISFRGMKSE 9
           I+FRGM+++
Sbjct: 190 ITFRGMENK 198
>XP_005250682
          Length = 204

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISFRGMKSE 9
           I+FRGM+++
Sbjct: 190 ITFRGMENK 198
>NP_835228
          Length = 388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SFRGMKSE 9
           +FRG+K E
Sbjct: 285 AFRGLKKE 292
>NP_005088
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           +FRG+KS
Sbjct: 134 TFRGLKS 140
>NP_060606
          Length = 3477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    SFRGMKS 8
            +FRGMK+
Sbjct: 2394 AFRGMKT 2400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    SFRGMK 7
            +FRGMK
Sbjct: 2748 AFRGMK 2753
>NP_004058
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           I  RG+KSE
Sbjct: 301 IEARGLKSE 309
>NP_001035025
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           I  RG+KSE
Sbjct: 165 IEARGLKSE 173
>NP_644807
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           +FRG+KS
Sbjct: 131 TFRGLKS 137
>NP_543138
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           ++FRG+K +
Sbjct: 199 VAFRGLKGK 207
>NP_079382
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISFRGMKSE 9
           ++FRG+K +
Sbjct: 199 VAFRGLKGK 207
>NP_112598
          Length = 5088

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    ISFRGMKSE 9
            +SFRG++ +
Sbjct: 2708 VSFRGLRRQ 2716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    ISFRGMKSE 9
            +SFRG++ +
Sbjct: 3239 VSFRGLRCQ 3247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    ISFRGMKSE 9
            +SFRG++ +
Sbjct: 3773 VSFRGLRRQ 3781

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    ISFRGMKSE 9
            +SFRG++ +
Sbjct: 4307 VSFRGLRRQ 4315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    ISFRGMKSE 9
            +SFRG++ +
Sbjct: 4840 VSFRGLRRQ 4848
>XP_005270329
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           +FRG+KS
Sbjct: 134 TFRGLKS 140
>XP_005249659
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           I  RG+KSE
Sbjct: 94  IEARGLKSE 102
>XP_005249656
          Length = 287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           I  RG+KSE
Sbjct: 120 IEARGLKSE 128
>XP_005273067
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           +SFRG + E
Sbjct: 201 VSFRGQRIE 209
>NP_006606
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           +SFRG + E
Sbjct: 264 VSFRGQRIE 272
>NP_057536
          Length = 664

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           +SFRG + E
Sbjct: 264 VSFRGQRIE 272
>NP_000245
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           SFRGM++
Sbjct: 583 SFRGMEA 589
>NP_001035351
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SFRGMK 7
           SFRG+K
Sbjct: 364 SFRGLK 369
>NP_001035350
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SFRGMK 7
           SFRG+K
Sbjct: 347 SFRGLK 352
>NP_001846
          Length = 1388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ISFRGMKSE 9
           I+F G+K E
Sbjct: 980 ITFHGVKGE 988
>NP_942014
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FRGMKSE 9
           FRG+K E
Sbjct: 617 FRGVKGE 623
>NP_001138899
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FRGMKS 8
           F+GMKS
Sbjct: 289 FKGMKS 294
>NP_001096045
          Length = 624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FRGMKS 8
           F+GMKS
Sbjct: 289 FKGMKS 294
>NP_443102
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FRGMKS 8
           F+GMKS
Sbjct: 289 FKGMKS 294
>XP_005266846
          Length = 135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FRGMKSE 9
          +RGMK+E
Sbjct: 8  WRGMKNE 14
>XP_005266845
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FRGMKSE 9
          +RGMK+E
Sbjct: 43 WRGMKNE 49
>XP_005266844
          Length = 179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FRGMKSE 9
          +RGMK+E
Sbjct: 52 WRGMKNE 58
>XP_005262843
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SFRGMK 7
           SFRG+K
Sbjct: 347 SFRGLK 352
>XP_005262842
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SFRGMK 7
           SFRG+K
Sbjct: 364 SFRGLK 369
>XP_005273202
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SFRGMKS 8
          SFRGM++
Sbjct: 37 SFRGMEA 43
>XP_006711833
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           SFRGM++
Sbjct: 271 SFRGMEA 277
>XP_006711832
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           SFRGM++
Sbjct: 583 SFRGMEA 589
>XP_005273200
          Length = 1214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           SFRGM++
Sbjct: 583 SFRGMEA 589
>XP_005273198
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFRGMKS 8
           SFRGM++
Sbjct: 582 SFRGMEA 588
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.133    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 428,379
Number of extensions: 613
Number of successful extensions: 59
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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