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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001276866                                                           18    1935
NP_004439                                                              18    1935
NP_001276865                                                           18    1935
NP_001005862                                                           18    1935
XP_005257197                                                           18    1935
XP_006721828                                                           18    1935
NP_000254                                                              17    2528
NP_899200                                                              16    5631
XP_005247134                                                           16    5631
NP_005290                                                              16    7355
NP_666018                                                              16    7355
NP_001138135                                                           15    9605
NP_004054                                                              15    9605
NP_001296                                                              15    9605
NP_060575                                                              15    9605

>NP_001276866
          Length = 1055

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           ISA VGILL
Sbjct: 655 ISAVVGILL 663



>NP_004439
          Length = 1255

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           ISA VGILL
Sbjct: 655 ISAVVGILL 663



>NP_001276865
          Length = 1240

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           ISA VGILL
Sbjct: 640 ISAVVGILL 648



>NP_001005862
          Length = 1225

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           ISA VGILL
Sbjct: 625 ISAVVGILL 633



>XP_005257197
          Length = 1225

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           ISA VGILL
Sbjct: 625 ISAVVGILL 633



>XP_006721828
          Length = 1336

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           ISA VGILL
Sbjct: 736 ISAVVGILL 744



>NP_000254
          Length = 743

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ISAAVGILL 9
          ++AAVG+LL
Sbjct: 6  VAAAVGVLL 14



>NP_899200
          Length = 1261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISAAVGILL 9
           ++AAVG++L
Sbjct: 274 LAAAVGVIL 282



>XP_005247134
          Length = 1286

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ISAAVGILL 9
           ++AAVG++L
Sbjct: 274 LAAAVGVIL 282



>NP_005290
          Length = 319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ISAAVGILL 9
          ++ AVG+LL
Sbjct: 13 VATAVGVLL 21



>NP_666018
          Length = 610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ISAAVGI 7
          ISAA+GI
Sbjct: 24 ISAAIGI 30



>NP_001138135
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISAAVGILL 9
           +  AVGILL
Sbjct: 785 VGMAVGILL 793



>NP_004054
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ISAAVGILL 9
           +  AVGILL
Sbjct: 785 VGMAVGILL 793



>NP_001296
          Length = 209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ISAAVGILL 9
          I AA+G+LL
Sbjct: 89 IVAALGVLL 97



>NP_060575
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ISAAVGILL 9
           + AA+G+L+
Sbjct: 112 VDAAIGLLM 120



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.147    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 333,934
Number of extensions: 294
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)