Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060712                                                              17    4312
NP_001121367                                                           17    4312
NP_001121368                                                           17    4312
NP_071912                                                              17    4312
NP_660207                                                              16    7355
NP_001071096                                                           16    7355
NP_057106                                                              16    7355
NP_001230700                                                           16    7355
NP_001001671                                                           16    7355
NP_001230701                                                           16    7355
XP_005269600                                                           16    7355
XP_006711425                                                           16    7355
NP_000675                                                              15    9605
NP_001265713                                                           15    9605
NP_001430                                                              15    9605
NP_001248370                                                           15    9605
NP_001248371                                                           15    9605
NP_001248369                                                           15    9605
NP_001028197                                                           15    9605
NP_005818                                                              15    9605
NP_000031                                                              15    9605
XP_005256986                                                           15    9605
XP_006721695                                                           15    9605
XP_005270679                                                           15    9605
XP_005270678                                                           15    9605
>NP_060712
          Length = 570

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          +R LLV+AG
Sbjct: 35 LRALLVLAG 43
>NP_001121367
          Length = 424

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RVLLVIAG 9
           RVL+V+AG
Sbjct: 207 RVLMVLAG 214
>NP_001121368
          Length = 424

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RVLLVIAG 9
           RVL+V+AG
Sbjct: 207 RVLMVLAG 214
>NP_071912
          Length = 424

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   RVLLVIAG 9
           RVL+V+AG
Sbjct: 207 RVLMVLAG 214
>NP_660207
          Length = 217

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLLVIAG 9
           R+LLVI G
Sbjct: 192 RILLVILG 199
>NP_001071096
          Length = 265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  IRVLLVIAG 9
          +RV++++AG
Sbjct: 30 LRVIILVAG 38
>NP_057106
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  IRVLLVIAG 9
          +RV++++AG
Sbjct: 30 LRVIILVAG 38
>NP_001230700
          Length = 190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  IRVLLVIAG 9
          +RV++++AG
Sbjct: 30 LRVIILVAG 38
>NP_001001671
          Length = 1313

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IRVLLVIAG 9
           + VLL++AG
Sbjct: 406 LAVLLIVAG 414
>NP_001230701
          Length = 195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  IRVLLVIAG 9
          +RV++++AG
Sbjct: 30 LRVIILVAG 38
>XP_005269600
          Length = 224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RVLLVIAG 9
           R+LLVI G
Sbjct: 199 RILLVILG 206
>XP_006711425
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  IRVLLVIAG 9
          +RV++++AG
Sbjct: 30 LRVIILVAG 38
>NP_000675
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLLVIAG 9
          VLL++AG
Sbjct: 69 VLLIVAG 75
>NP_001265713
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLLVIAG 9
          VLL++AG
Sbjct: 77 VLLIVAG 83
>NP_001430
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 26 ILVLLLVAG 34
>NP_001248370
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 34 ILVLLLVAG 42
>NP_001248371
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 26 ILVLLLVAG 34
>NP_001248369
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 26 ILVLLLVAG 34
>NP_001028197
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 26 ILVLLLVAG 34
>NP_005818
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VLLVIAG 9
           VLL++AG
Sbjct: 181 VLLIVAG 187
>NP_000031
          Length = 99

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  RVLLVIA 8
          RVLLV+A
Sbjct: 4  RVLLVVA 10
>XP_005256986
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLLVIAG 9
          VLL++AG
Sbjct: 77 VLLIVAG 83
>XP_006721695
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VLLVIAG 9
           VLL++AG
Sbjct: 215 VLLIVAG 221
>XP_005270679
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 26 ILVLLLVAG 34
>XP_005270678
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IRVLLVIAG 9
          I VLL++AG
Sbjct: 26 ILVLLLVAG 34
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.166    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 273,389
Number of extensions: 241
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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