Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005849                                                              17    3301
NP_001138882                                                           17    3301
NP_001138880                                                           17    3301
XP_005259763                                                           17    3301
NP_031389                                                              17    3301
NP_001138881                                                           17    3301
NP_056370                                                              17    4312
NP_001026794                                                           17    4312
NP_061159                                                              17    4312
NP_001273428                                                           17    4312
NP_001273425                                                           17    4312
NP_079325                                                              17    4312
NP_001007541                                                           17    4312
NP_001273426                                                           17    4312
NP_001073918                                                           17    4312
XP_005255915                                                           17    4312
XP_005254625                                                           17    4312
XP_005254624                                                           17    4312
XP_006717743                                                           17    4312
XP_005249051                                                           17    4312
NP_003811                                                              16    5631
NP_002717                                                              16    5631
NP_009224                                                              16    5631
NP_060060                                                              16    5631
NP_001239133                                                           16    5631
NP_001002029                                                           16    5631
NP_001229752                                                           16    5631
XP_006725011                                                           16    5631
XP_006725010                                                           16    5631
XP_005267101                                                           16    5631
XP_005265873                                                           16    5631
XP_005265872                                                           16    5631
XP_005245315                                                           16    5631
XP_006711447                                                           16    5631
XP_005245314                                                           16    5631
XP_006711083                                                           16    5631
XP_006711082                                                           16    5631
NP_002637                                                              16    5631
NP_114114                                                              16    5631
NP_776216                                                              16    7355
NP_006776                                                              16    7355
NP_000303                                                              16    7355
XP_006712696                                                           16    7355
XP_005263774                                                           16    7355
XP_005263773                                                           16    7355
XP_005263772                                                           16    7355
NP_004102                                                              15    9605
NP_973726                                                              15    9605
NP_001186148                                                           15    9605
XP_006718748                                                           15    9605
XP_006718747                                                           15    9605
XP_006718746                                                           15    9605
XP_006718745                                                           15    9605
NP_006765                                                              15    9605
>NP_005849
          Length = 692

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVKFACGV 9
           R++FAC V
Sbjct: 387 RIQFACSV 394
>NP_001138882
          Length = 382

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IRVKFAC 7
          +RV+FAC
Sbjct: 50 LRVRFAC 56
>NP_001138880
          Length = 471

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IRVKFAC 7
           +RV+FAC
Sbjct: 139 LRVRFAC 145
>XP_005259763
          Length = 679

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVKFACGV 9
           R++FAC V
Sbjct: 374 RIQFACSV 381
>NP_031389
          Length = 471

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IRVKFAC 7
           +RV+FAC
Sbjct: 139 LRVRFAC 145
>NP_001138881
          Length = 471

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IRVKFAC 7
           +RV+FAC
Sbjct: 139 LRVRFAC 145
>NP_056370
          Length = 1013

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IRVKFACGV 9
           I++K+ CG+
Sbjct: 663 IKIKYFCGL 671
>NP_001026794
          Length = 1061

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IRVKFACGV 9
           I++K+ CG+
Sbjct: 663 IKIKYFCGL 671
>NP_061159
          Length = 1361

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   RVKFACG 8
           RV+F CG
Sbjct: 398 RVRFLCG 404
>NP_001273428
          Length = 588

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVKFACGV 9
           +V+F CGV
Sbjct: 460 QVRFLCGV 467
>NP_001273425
          Length = 641

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVKFACGV 9
           +V+F CGV
Sbjct: 513 QVRFLCGV 520
>NP_079325
          Length = 641

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVKFACGV 9
           +V+F CGV
Sbjct: 513 QVRFLCGV 520
>NP_001007541
          Length = 788

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IRVKFACG 8
           +++KF CG
Sbjct: 99  VQLKFTCG 106
>NP_001273426
          Length = 707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RVKFACGV 9
           +V+F CGV
Sbjct: 513 QVRFLCGV 520
>NP_001073918
          Length = 1060

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IRVKFACG 8
           +R+ +ACG
Sbjct: 662 VRILYACG 669
>XP_005255915
          Length = 1099

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RVKFACGV 9
          RV+F CG+
Sbjct: 25 RVEFLCGL 32
>XP_005254625
          Length = 1361

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   RVKFACG 8
           RV+F CG
Sbjct: 398 RVRFLCG 404
>XP_005254624
          Length = 1361

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   RVKFACG 8
           RV+F CG
Sbjct: 398 RVRFLCG 404
>XP_006717743
          Length = 1090

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IRVKFACG 8
           +R+ +ACG
Sbjct: 692 VRILYACG 699
>XP_005249051
          Length = 975

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IRVKFACGV 9
           I++K+ CG+
Sbjct: 663 IKIKYFCGL 671
>NP_003811
          Length = 283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RVKFACG 8
          RVK ACG
Sbjct: 62 RVKEACG 68
>NP_002717
          Length = 710

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RVKFAC 7
           RVKF+C
Sbjct: 170 RVKFSC 175
>NP_009224
          Length = 1744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RVKFAC 7
            R+KFAC
Sbjct: 1613 RMKFAC 1618
>NP_060060
          Length = 977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   IRVKFACG 8
           +++KF CG
Sbjct: 691 LQMKFVCG 698
>NP_001239133
          Length = 1698

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RVKFAC 7
            R+KFAC
Sbjct: 1567 RMKFAC 1572
>NP_001002029
          Length = 1744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RVKFAC 7
            R+KFAC
Sbjct: 1613 RMKFAC 1618
>NP_001229752
          Length = 1744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    RVKFAC 7
            R+KFAC
Sbjct: 1613 RMKFAC 1618
>XP_006725011
          Length = 193

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RVKFACG 8
          RVK ACG
Sbjct: 62 RVKEACG 68
>XP_006725010
          Length = 197

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RVKFACG 8
          RVK ACG
Sbjct: 62 RVKEACG 68
>XP_005267101
          Length = 449

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RVKFAC 7
           RVKF+C
Sbjct: 170 RVKFSC 175
>XP_005265873
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KFACGV 9
           KF CGV
Sbjct: 163 KFVCGV 168
>XP_005265872
          Length = 303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KFACGV 9
           KF CGV
Sbjct: 204 KFVCGV 209
>XP_005245315
          Length = 1607

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IRVKFACG 8
            IRV F CG
Sbjct: 1051 IRVIFKCG 1058
>XP_006711447
          Length = 1634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IRVKFACG 8
            IRV F CG
Sbjct: 1078 IRVIFKCG 1085
>XP_005245314
          Length = 1634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IRVKFACG 8
            IRV F CG
Sbjct: 1078 IRVIFKCG 1085
>XP_006711083
          Length = 193

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RVKFACG 8
          RVK ACG
Sbjct: 62 RVKEACG 68
>XP_006711082
          Length = 197

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RVKFACG 8
          RVK ACG
Sbjct: 62 RVKEACG 68
>NP_002637
          Length = 1634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    IRVKFACG 8
            IRV F CG
Sbjct: 1078 IRVIFKCG 1085
>NP_114114
          Length = 330

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KFACGV 9
           KF CGV
Sbjct: 231 KFVCGV 236
>NP_776216
          Length = 813

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VKFACGV 9
           +KF CGV
Sbjct: 581 LKFDCGV 587
>NP_006776
          Length = 824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VKFACGV 9
           +KF CGV
Sbjct: 592 LKFDCGV 598
>NP_000303
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VKFACG 8
           VKF CG
Sbjct: 179 VKFPCG 184
>XP_006712696
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VKFACG 8
           VKF CG
Sbjct: 187 VKFPCG 192
>XP_005263774
          Length = 482

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VKFACG 8
           VKF CG
Sbjct: 200 VKFPCG 205
>XP_005263773
          Length = 516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VKFACG 8
           VKF CG
Sbjct: 234 VKFPCG 239
>XP_005263772
          Length = 522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   VKFACG 8
           VKF CG
Sbjct: 240 VKFPCG 245
>NP_004102
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 3   VKFACG 8
           +KF CG
Sbjct: 307 IKFMCG 312
>NP_973726
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   KFACGV 9
           K+ACG+
Sbjct: 454 KYACGL 459
>NP_001186148
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   VKFAC 7
           VKFAC
Sbjct: 110 VKFAC 114
>XP_006718748
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   KFACGV 9
           K+ACG+
Sbjct: 458 KYACGL 463
>XP_006718747
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   KFACGV 9
           K+ACG+
Sbjct: 455 KYACGL 460
>XP_006718746
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   KFACGV 9
           K+ACG+
Sbjct: 458 KYACGL 463
>XP_006718745
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   KFACGV 9
           K+ACG+
Sbjct: 458 KYACGL 463
>NP_006765
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   VKFAC 7
           VKFAC
Sbjct: 111 VKFAC 115
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.154    0.522 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 554,309
Number of extensions: 647
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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