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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           19     869
NP_060785                                                              19     869
NP_361014                                                              19     869
XP_006710739                                                           17    3301
XP_005270979                                                           17    3301
NP_056972                                                              17    3301
NP_001750                                                              16    5631
XP_005253870                                                           16    5631
NP_056171                                                              16    7355
NP_874365                                                              16    7355
NP_000065                                                              16    7355
NP_056993                                                              16    7355
NP_001127880                                                           15    9605
NP_000824                                                              15    9605
NP_001127879                                                           15    9605
NP_005151                                                              15    9605
XP_006724221                                                           15    9605
XP_005255325                                                           15    9605
XP_005255324                                                           15    9605
NP_065172                                                              15    9605

>NP_001013433
          Length = 589

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           I+NLVTLES
Sbjct: 539 IRNLVTLES 547



>NP_060785
          Length = 627

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           I+NLVTLES
Sbjct: 577 IRNLVTLES 585



>NP_361014
          Length = 707

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           I+NLVTLES
Sbjct: 657 IRNLVTLES 665



>XP_006710739
          Length = 560

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IQNLVTLE 8
           IQN++TLE
Sbjct: 138 IQNIMTLE 145



>XP_005270979
          Length = 728

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IQNLVTLE 8
           IQN++TLE
Sbjct: 138 IQNIMTLE 145



>NP_056972
          Length = 728

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IQNLVTLE 8
           IQN++TLE
Sbjct: 138 IQNIMTLE 145



>NP_001750
          Length = 289

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IQNLVTLE 8
          +QNL+T+E
Sbjct: 27 LQNLLTIE 34



>XP_005253870
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  IQNLVTLE 8
          +QNL+T+E
Sbjct: 27 LQNLLTIE 34



>NP_056171
          Length = 1630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IQNLVTLE 8
           + NLVTLE
Sbjct: 150 LANLVTLE 157



>NP_874365
          Length = 1655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IQNLVTLE 8
           + NLVTLE
Sbjct: 150 LANLVTLE 157



>NP_000065
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   NLVTLES 9
           NLVTLE+
Sbjct: 151 NLVTLEN 157



>NP_056993
          Length = 2371

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   NLVTLES 9
           NLVTLE+
Sbjct: 884 NLVTLET 890



>NP_001127880
          Length = 1281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           +QN++TL++
Sbjct: 200 MQNVITLDT 208



>NP_000824
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           +QN++TL++
Sbjct: 200 MQNVITLDT 208



>NP_001127879
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           +QN++TL++
Sbjct: 200 MQNVITLDT 208



>NP_005151
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QNLVTLE 8
           QNL+T+E
Sbjct: 598 QNLLTME 604



>XP_006724221
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   QNLVTLE 8
           QNL+T+E
Sbjct: 485 QNLLTME 491



>XP_005255325
          Length = 1333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           +QN++TL++
Sbjct: 252 MQNVITLDT 260



>XP_005255324
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IQNLVTLES 9
           +QN++TL++
Sbjct: 252 MQNVITLDT 260



>NP_065172
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  QNLVTLE 8
          +N+VTLE
Sbjct: 78 ENIVTLE 84



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.126    0.291 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 347,623
Number of extensions: 460
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)