Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1482
XP_006718908                                                           18    1482
XP_005271621                                                           18    1482
XP_006718907                                                           18    1482
XP_006718906                                                           18    1482
XP_005271619                                                           18    1482
XP_005271618                                                           18    1482
NP_073754                                                              17    3301
NP_004777                                                              16    5631
NP_002798                                                              16    5631
NP_001177966                                                           16    5631
NP_149100                                                              16    5631
XP_006722644                                                           16    5631
XP_006722643                                                           16    5631
XP_005264686                                                           16    5631
NP_076994                                                              16    7355
XP_005254133                                                           16    7355
XP_005254132                                                           16    7355
XP_005254131                                                           16    7355
NP_005899                                                              15    9605
>NP_000042
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IMVKLFVNL 9
            IMVKL VNL
Sbjct: 1643 IMVKLVVNL 1651
>XP_006718908
          Length = 1708

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IMVKLFVNL 9
           IMVKL VNL
Sbjct: 295 IMVKLVVNL 303
>XP_005271621
          Length = 2708

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IMVKLFVNL 9
            IMVKL VNL
Sbjct: 1295 IMVKLVVNL 1303
>XP_006718907
          Length = 3050

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IMVKLFVNL 9
            IMVKL VNL
Sbjct: 1637 IMVKLVVNL 1645
>XP_006718906
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IMVKLFVNL 9
            IMVKL VNL
Sbjct: 1643 IMVKLVVNL 1651
>XP_005271619
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IMVKLFVNL 9
            IMVKL VNL
Sbjct: 1643 IMVKLVVNL 1651
>XP_005271618
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    IMVKLFVNL 9
            IMVKL VNL
Sbjct: 1643 IMVKLVVNL 1651
>NP_073754
          Length = 951

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IMVKLFVN 8
           +MV LFVN
Sbjct: 837 VMVNLFVN 844
>NP_004777
          Length = 289

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VKLFVNL 9
           VK+F+NL
Sbjct: 194 VKIFINL 200
>NP_002798
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IMVKLFVNL 9
           ++VK+++NL
Sbjct: 200 VLVKIYMNL 208
>NP_001177966
          Length = 922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IMVKLFVNL 9
           ++VK+++NL
Sbjct: 200 VLVKIYMNL 208
>NP_149100
          Length = 783

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IMVKLFVNL 9
           +MVKL+ N+
Sbjct: 694 VMVKLYPNM 702
>XP_006722644
          Length = 255

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VKLFVNL 9
           VK+F+NL
Sbjct: 194 VKIFINL 200
>XP_006722643
          Length = 292

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VKLFVNL 9
           VK+F+NL
Sbjct: 194 VKIFINL 200
>XP_005264686
          Length = 703

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IMVKLFVNL 9
           +MVKL+ N+
Sbjct: 614 VMVKLYPNM 622
>NP_076994
          Length = 502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VKLFVNL 9
           V+LFVNL
Sbjct: 230 VELFVNL 236
>XP_005254133
          Length = 283

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  VKLFVNL 9
          V+LFVNL
Sbjct: 11 VELFVNL 17
>XP_005254132
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VKLFVNL 9
           V+LFVNL
Sbjct: 230 VELFVNL 236
>XP_005254131
          Length = 476

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VKLFVNL 9
           V+LFVNL
Sbjct: 204 VELFVNL 210
>NP_005899
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MVKLFVNL 9
           +V+LFVN+
Sbjct: 274 IVELFVNI 281
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.350    0.160    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 293,995
Number of extensions: 314
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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