Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1482
NP_001263689                                                           18    1482
NP_001119584                                                           18    1482
NP_001119586                                                           18    1482
NP_001263625                                                           18    1482
NP_001119590                                                           18    1482
NP_001263690                                                           18    1482
NP_001119585                                                           18    1482
NP_001263624                                                           18    1482
NP_001119588                                                           18    1482
NP_001119587                                                           18    1482
NP_001119589                                                           18    1482
NP_001263627                                                           18    1482
NP_001263628                                                           18    1482
NP_001263626                                                           18    1482
NP_001239553                                                           16    7355
NP_005206                                                              16    7355
NP_001106272                                                           16    7355
NP_001106271                                                           16    7355
NP_066920                                                              16    7355
XP_005258261                                                           16    7355
XP_006712148                                                           16    7355
XP_006712147                                                           16    7355
XP_006712146                                                           16    7355
XP_006712145                                                           16    7355
XP_006712144                                                           16    7355
XP_006712143                                                           16    7355
XP_005264569                                                           16    7355
NP_001158159                                                           15    9605
NP_062873                                                              15    9605
NP_001158157                                                           15    9605
NP_062874                                                              15    9605
NP_001277024                                                           15    9605
XP_006725081                                                           15    9605
XP_006725080                                                           15    9605
XP_006715539                                                           15    9605
XP_005267031                                                           15    9605
NP_001010923                                                           15    9605
NP_000863                                                              15    9605
>NP_000537
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 251 ILTIITLED 259
>NP_001263689
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 212 ILTIITLED 220
>NP_001119584
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 251 ILTIITLED 259
>NP_001119586
          Length = 341

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 251 ILTIITLED 259
>NP_001263625
          Length = 302

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 212 ILTIITLED 220
>NP_001119590
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 212 ILTIITLED 220
>NP_001263690
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 212 ILTIITLED 220
>NP_001119585
          Length = 346

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 251 ILTIITLED 259
>NP_001263624
          Length = 307

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 212 ILTIITLED 220
>NP_001119588
          Length = 209

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 119 ILTIITLED 127
>NP_001119587
          Length = 261

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 119 ILTIITLED 127
>NP_001119589
          Length = 214

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 119 ILTIITLED 127
>NP_001263627
          Length = 182

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 92  ILTIITLED 100
>NP_001263628
          Length = 187

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 92  ILTIITLED 100
>NP_001263626
          Length = 234

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ILTIITLAD 9
           ILTIITL D
Sbjct: 92  ILTIITLED 100
>NP_001239553
          Length = 965

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 666 TVITIAD 672
>NP_005206
          Length = 1447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           +T++TL+D
Sbjct: 613 ITVVTLSD 620
>NP_001106272
          Length = 960

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 661 TVITIAD 667
>NP_001106271
          Length = 968

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 669 TVITIAD 675
>NP_066920
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 674 TVITIAD 680
>XP_005258261
          Length = 1447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           +T++TL+D
Sbjct: 613 ITVVTLSD 620
>XP_006712148
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 674 TVITIAD 680
>XP_006712147
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 674 TVITIAD 680
>XP_006712146
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 674 TVITIAD 680
>XP_006712145
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 674 TVITIAD 680
>XP_006712144
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 674 TVITIAD 680
>XP_006712143
          Length = 996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 697 TVITIAD 703
>XP_005264569
          Length = 1003

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIITLAD 9
           T+IT+AD
Sbjct: 704 TVITIAD 710
>NP_001158159
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 533 LTLLTLAE 540
>NP_062873
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ILTIITL 7
          ILT+ITL
Sbjct: 87 ILTLITL 93
>NP_001158157
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 568 LTLLTLAE 575
>NP_062874
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ILTIITL 7
          ILT+ITL
Sbjct: 87 ILTLITL 93
>NP_001277024
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ILTIITLA 8
           +LT++TLA
Sbjct: 99  LLTMVTLA 106
>XP_006725081
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 471 LTLLTLAE 478
>XP_006725080
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 533 LTLLTLAE 540
>XP_006715539
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 471 LTLLTLAE 478
>XP_005267031
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 533 LTLLTLAE 540
>NP_001010923
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LTIITLAD 9
           LT++TLA+
Sbjct: 568 LTLLTLAE 575
>NP_000863
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  ILTIITL 7
          ILT+ITL
Sbjct: 87 ILTLITL 93
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.151    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,817
Number of extensions: 514
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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