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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057573                                                              16    5631
NP_001159590                                                           16    5631
NP_001002010                                                           16    5631
NP_001002009                                                           16    5631
NP_006454                                                              16    7355
NP_998787                                                              16    7355
NP_964010                                                              16    7355
XP_005264145                                                           16    7355
XP_006711984                                                           16    7355
XP_006711983                                                           16    7355
NP_689560                                                              15    9605
XP_005274129                                                           15    9605
XP_006718655                                                           15    9605
XP_005274128                                                           15    9605
XP_005274127                                                           15    9605
NP_060327                                                              15    9605

>NP_057573
          Length = 297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILRQASVY 8
           ++RQA VY
Sbjct: 174 VIRQAGVY 181



>NP_001159590
          Length = 285

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILRQASVY 8
           ++RQA VY
Sbjct: 162 VIRQAGVY 169



>NP_001002010
          Length = 336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILRQASVY 8
           ++RQA VY
Sbjct: 213 VIRQAGVY 220



>NP_001002009
          Length = 297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ILRQASVY 8
           ++RQA VY
Sbjct: 174 VIRQAGVY 181



>NP_006454
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILRQASVY 8
          I+R AS+Y
Sbjct: 43 IIRMASIY 50



>NP_998787
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILRQASVY 8
          I+R AS+Y
Sbjct: 43 IIRMASIY 50



>NP_964010
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILRQASVY 8
          I+R AS+Y
Sbjct: 43 IIRMASIY 50



>XP_005264145
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILRQASVY 8
          I+R AS+Y
Sbjct: 43 IIRMASIY 50



>XP_006711984
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILRQASVY 8
          I+R AS+Y
Sbjct: 90 IIRMASIY 97



>XP_006711983
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ILRQASVY 8
          I+R AS+Y
Sbjct: 90 IIRMASIY 97



>NP_689560
          Length = 973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RQASVYM 9
           RQA VYM
Sbjct: 918 RQAVVYM 924



>XP_005274129
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ILRQASVY 8
           ++RQASV+
Sbjct: 553 VVRQASVH 560



>XP_006718655
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ILRQASVY 8
           ++RQASV+
Sbjct: 581 VVRQASVH 588



>XP_005274128
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ILRQASVY 8
           ++RQASV+
Sbjct: 590 VVRQASVH 597



>XP_005274127
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ILRQASVY 8
           ++RQASV+
Sbjct: 653 VVRQASVH 660



>NP_060327
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ILRQASVY 8
           ++RQASV+
Sbjct: 644 VVRQASVH 651



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.138    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 332,862
Number of extensions: 312
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)