Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_001254500                                                           17    2528
NP_001254501                                                           17    2528
NP_060891                                                              17    2528
NP_573444                                                              17    4312
NP_078802                                                              17    4312
NP_620641                                                              17    4312
XP_006710957                                                           17    4312
NP_001265371                                                           15    9605
NP_004508                                                              15    9605
NP_001014795                                                           15    9605
NP_001014794                                                           15    9605
NP_001265370                                                           15    9605
NP_057937                                                              15    9605
NP_057941                                                              15    9605
NP_057936                                                              15    9605
NP_000793                                                              15    9605
XP_005252962                                                           15    9605
XP_005252961                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IKLMNVAMN 9
           IKLMNVA+N
Sbjct: 488 IKLMNVAIN 496
>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IKLMNVAMN 9
           IKLMNVA+N
Sbjct: 488 IKLMNVAIN 496
>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IKLMNVAMN 9
           IKLMNVA+N
Sbjct: 488 IKLMNVAIN 496
>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IKLMNVAMN 9
           IKLMNVA+N
Sbjct: 488 IKLMNVAIN 496
>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IKLMNVAMN 9
           IKLMNVA+N
Sbjct: 488 IKLMNVAIN 496
>NP_001254500
          Length = 928

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLMNVAMN 9
           KLMN+A N
Sbjct: 861 KLMNIAFN 868
>NP_001254501
          Length = 468

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLMNVAMN 9
           KLMN+A N
Sbjct: 401 KLMNIAFN 408
>NP_060891
          Length = 917

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KLMNVAMN 9
           KLMN+A N
Sbjct: 850 KLMNIAFN 857
>NP_573444
          Length = 593

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IKLMNVAMN 9
           I+ MNVA+N
Sbjct: 96  IQAMNVALN 104
>NP_078802
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IKLMNVAMN 9
           I+ MNVA+N
Sbjct: 96  IQAMNVALN 104
>NP_620641
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IKLMNVAMN 9
           I+ MNVA+N
Sbjct: 96  IQAMNVALN 104
>XP_006710957
          Length = 564

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IKLMNVAMN 9
           I+ MNVA+N
Sbjct: 96  IQAMNVALN 104
>NP_001265371
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 289 KLMKICMN 296
>NP_004508
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 423 KLMKICMN 430
>NP_001014795
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 423 KLMKICMN 430
>NP_001014794
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 423 KLMKICMN 430
>NP_001265370
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 362 KLMKICMN 369
>NP_057937
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KLMNVAMN 9
          +L+NV MN
Sbjct: 32 ELLNVCMN 39
>NP_057941
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KLMNVAMN 9
          +L+NV MN
Sbjct: 32 ELLNVCMN 39
>NP_057936
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KLMNVAMN 9
          +L+NV MN
Sbjct: 32 ELLNVCMN 39
>NP_000793
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KLMNVAMN 9
          +L+NV MN
Sbjct: 32 ELLNVCMN 39
>XP_005252962
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 289 KLMKICMN 296
>XP_005252961
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLMNVAMN 9
           KLM + MN
Sbjct: 423 KLMKICMN 430
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.132    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 249,838
Number of extensions: 219
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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