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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001276866                                                           18    1935
NP_004439                                                              18    1935
NP_001276865                                                           18    1935
NP_001005862                                                           18    1935
XP_005257197                                                           18    1935
XP_006721828                                                           18    1935
NP_666018                                                              17    3301
NP_000254                                                              16    7355
NP_899200                                                              16    7355
XP_005247134                                                           16    7355

>NP_001276866
          Length = 1055

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IISAAVGIL 9
           IISA VGIL
Sbjct: 654 IISAVVGIL 662



>NP_004439
          Length = 1255

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IISAAVGIL 9
           IISA VGIL
Sbjct: 654 IISAVVGIL 662



>NP_001276865
          Length = 1240

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IISAAVGIL 9
           IISA VGIL
Sbjct: 639 IISAVVGIL 647



>NP_001005862
          Length = 1225

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IISAAVGIL 9
           IISA VGIL
Sbjct: 624 IISAVVGIL 632



>XP_005257197
          Length = 1225

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IISAAVGIL 9
           IISA VGIL
Sbjct: 624 IISAVVGIL 632



>XP_006721828
          Length = 1336

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IISAAVGIL 9
           IISA VGIL
Sbjct: 735 IISAVVGIL 743



>NP_666018
          Length = 610

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  IISAAVGI 8
          +ISAA+GI
Sbjct: 23 VISAAIGI 30



>NP_000254
          Length = 743

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ISAAVGIL 9
          ++AAVG+L
Sbjct: 6  VAAAVGVL 13



>NP_899200
          Length = 1261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IISAAVGIL 9
           +++AAVG++
Sbjct: 273 VLAAAVGVI 281



>XP_005247134
          Length = 1286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IISAAVGIL 9
           +++AAVG++
Sbjct: 273 VLAAAVGVI 281



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.149    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 317,527
Number of extensions: 258
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)