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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     509
NP_000242                                                              20     509
XP_005264389                                                           20     509
NP_001118230                                                           17    3301
XP_006721512                                                           17    3301
XP_006721511                                                           17    3301
NP_006614                                                              16    5631
NP_056996                                                              15    9605
NP_065186                                                              15    9605
XP_005250985                                                           15    9605
XP_006716634                                                           15    9605
XP_005248177                                                           15    9605
XP_005248176                                                           15    9605
XP_005248175                                                           15    9605

>NP_001245210
          Length = 868

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IIRSATKDS 9
           I+RSATKDS
Sbjct: 669 ILRSATKDS 677



>NP_000242
          Length = 934

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IIRSATKDS 9
           I+RSATKDS
Sbjct: 735 ILRSATKDS 743



>XP_005264389
          Length = 921

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IIRSATKDS 9
           I+RSATKDS
Sbjct: 735 ILRSATKDS 743



>NP_001118230
          Length = 549

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IIRSATKDS 9
           +IR +TKDS
Sbjct: 485 LIRQSTKDS 493



>XP_006721512
          Length = 379

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IIRSATKDS 9
           +IR +TKDS
Sbjct: 315 LIRQSTKDS 323



>XP_006721511
          Length = 398

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IIRSATKDS 9
           +IR +TKDS
Sbjct: 334 LIRQSTKDS 342



>NP_006614
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  IIRSATK 7
          I+RSATK
Sbjct: 52 IVRSATK 58



>NP_056996
          Length = 1406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRSATKDS 9
           IRS+ KDS
Sbjct: 654 IRSSLKDS 661



>NP_065186
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IIRSATKD 8
          +IR AT+D
Sbjct: 15 LIRGATRD 22



>XP_005250985
          Length = 1406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRSATKDS 9
           IRS+ KDS
Sbjct: 654 IRSSLKDS 661



>XP_006716634
          Length = 1420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRSATKDS 9
           IRS+ KDS
Sbjct: 668 IRSSLKDS 675



>XP_005248177
          Length = 1399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IIRSATKD 8
          +IR AT+D
Sbjct: 15 LIRGATRD 22



>XP_005248176
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IIRSATKD 8
          +IR AT+D
Sbjct: 15 LIRGATRD 22



>XP_005248175
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  IIRSATKD 8
          +IR AT+D
Sbjct: 15 LIRGATRD 22



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.120    0.280 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 360,764
Number of extensions: 436
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)