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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     665
NP_056081                                                              16    5631
NP_001247420                                                           16    5631
XP_006723819                                                           16    5631
NP_001026                                                              15    9605
NP_001276866                                                           15    9605
NP_004439                                                              15    9605
NP_001276865                                                           15    9605
NP_001005862                                                           15    9605
NP_001230925                                                           15    9605
NP_001265524                                                           15    9605
NP_877577                                                              15    9605
NP_005907                                                              15    9605
NP_001027                                                              15    9605
NP_689841                                                              15    9605
XP_005257197                                                           15    9605
XP_006721828                                                           15    9605
XP_006716579                                                           15    9605
XP_005250871                                                           15    9605
XP_006716578                                                           15    9605
XP_005250870                                                           15    9605
XP_006716577                                                           15    9605
XP_005250869                                                           15    9605
XP_005250868                                                           15    9605
XP_005250405                                                           15    9605
XP_006711873                                                           15    9605
XP_006711872                                                           15    9605
XP_006711871                                                           15    9605
XP_006711870                                                           15    9605
XP_006711869                                                           15    9605
XP_006711868                                                           15    9605
XP_006711867                                                           15    9605
XP_006711866                                                           15    9605
XP_006711865                                                           15    9605

>NP_005219
          Length = 1210

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IIEFSKMAQ 9
           IIEFSKMA+
Sbjct: 965 IIEFSKMAR 973



>NP_056081
          Length = 581

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IIEFSKMA 8
           IIEF K+A
Sbjct: 106 IIEFKKLA 113



>NP_001247420
          Length = 597

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IIEFSKMA 8
           IIEF K+A
Sbjct: 106 IIEFKKLA 113



>XP_006723819
          Length = 614

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   IIEFSKMA 8
           IIEF K+A
Sbjct: 106 IIEFKKLA 113



>NP_001026
          Length = 4967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>NP_001276866
          Length = 1055

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           + EFS+MA+
Sbjct: 973 VSEFSRMAR 981



>NP_004439
          Length = 1255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           + EFS+MA+
Sbjct: 973 VSEFSRMAR 981



>NP_001276865
          Length = 1240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           + EFS+MA+
Sbjct: 958 VSEFSRMAR 966



>NP_001005862
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           + EFS+MA+
Sbjct: 943 VSEFSRMAR 951



>NP_001230925
          Length = 4865

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 905 LVEFSKLPE 913



>NP_001265524
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IIEFSKMAQ 9
           I EF KMA+
Sbjct: 268 IDEFDKMAE 276



>NP_877577
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IIEFSKMAQ 9
           I EF KMA+
Sbjct: 268 IDEFDKMAE 276



>NP_005907
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IIEFSKMAQ 9
           I EF KMA+
Sbjct: 444 IDEFDKMAE 452



>NP_001027
          Length = 4870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 905 LVEFSKLPE 913



>NP_689841
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_005257197
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           + EFS+MA+
Sbjct: 943 VSEFSRMAR 951



>XP_006721828
          Length = 1336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IIEFSKMAQ 9
            + EFS+MA+
Sbjct: 1054 VSEFSRMAR 1062



>XP_006716579
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IEFSKMAQ 9
          +EF K+AQ
Sbjct: 12 VEFLKLAQ 19



>XP_005250871
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_006716578
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_005250870
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_006716577
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_005250869
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_005250868
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IEFSKMAQ 9
           +EF K+AQ
Sbjct: 99  VEFLKLAQ 106



>XP_005250405
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IIEFSKMAQ 9
           I EF KMA+
Sbjct: 337 IDEFDKMAE 345



>XP_006711873
          Length = 4954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711872
          Length = 2735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711871
          Length = 4906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711870
          Length = 4965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711869
          Length = 4973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711868
          Length = 4975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711867
          Length = 4977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711866
          Length = 4984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



>XP_006711865
          Length = 4985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   IIEFSKMAQ 9
           ++EFSK+ +
Sbjct: 918 LVEFSKLPE 926



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.134    0.323 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 349,964
Number of extensions: 454
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)