Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           19     665
NP_001020014                                                           19     665
NP_055903                                                              19     665
NP_689810                                                              17    4312
XP_006720481                                                           17    4312
XP_006720480                                                           17    4312
XP_005254266                                                           17    4312
XP_006720479                                                           17    4312
XP_005254265                                                           17    4312
NP_114032                                                              16    5631
NP_004492                                                              16    5631
NP_006717                                                              16    5631
NP_001093591                                                           16    5631
NP_001093590                                                           16    5631
NP_001186211                                                           16    5631
XP_005263433                                                           16    5631
XP_005263432                                                           16    5631
XP_005263431                                                           16    5631
XP_006714492                                                           16    5631
XP_005263430                                                           16    5631
XP_005263429                                                           16    5631
NP_001032401                                                           16    5631
NP_055508                                                              16    5631
NP_954592                                                              16    7355
NP_001229265                                                           16    7355
NP_001128509                                                           16    7355
XP_005259765                                                           16    7355
XP_006714485                                                           16    7355
XP_005263416                                                           16    7355
XP_006714483                                                           16    7355
XP_005263415                                                           16    7355
NP_005918                                                              16    7355
NP_005378                                                              15    9605
NP_624357                                                              15    9605
NP_624358                                                              15    9605
NP_057404                                                              15    9605
NP_003881                                                              15    9605
NP_056465                                                              15    9605
NP_149973                                                              15    9605
NP_001070817                                                           15    9605
NP_071423                                                              15    9605
NP_004897                                                              15    9605
NP_001257460                                                           15    9605
NP_149971                                                              15    9605
XP_006723857                                                           15    9605
XP_005260203                                                           15    9605
XP_006718305                                                           15    9605
XP_006718304                                                           15    9605
XP_006718303                                                           15    9605
XP_005253007                                                           15    9605
XP_006714730                                                           15    9605
XP_005272100                                                           15    9605
XP_006712476                                                           15    9605
XP_006712475                                                           15    9605
>NP_001155973
          Length = 1833

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IGSKSSNAN 9
            IGSKSSNA+
Sbjct: 1467 IGSKSSNAS 1475
>NP_001020014
          Length = 1029

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IGSKSSNAN 9
           IGSKSSNA+
Sbjct: 663 IGSKSSNAS 671
>NP_055903
          Length = 1723

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IGSKSSNAN 9
            IGSKSSNA+
Sbjct: 1357 IGSKSSNAS 1365
>NP_689810
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GSKSSNAN 9
          GS+SSNA+
Sbjct: 23 GSRSSNAS 30
>XP_006720481
          Length = 406

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GSKSSNAN 9
          GS+SSNA+
Sbjct: 23 GSRSSNAS 30
>XP_006720480
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GSKSSNAN 9
          GS+SSNA+
Sbjct: 23 GSRSSNAS 30
>XP_005254266
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GSKSSNAN 9
          GS+SSNA+
Sbjct: 23 GSRSSNAS 30
>XP_006720479
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GSKSSNAN 9
          GS+SSNA+
Sbjct: 23 GSRSSNAS 30
>XP_005254265
          Length = 581

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GSKSSNAN 9
          GS+SSNA+
Sbjct: 23 GSRSSNAS 30
>NP_114032
          Length = 825

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GSKSSNAN 9
           GSK SN N
Sbjct: 686 GSKKSNKN 693
>NP_004492
          Length = 806

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GSKSSNAN 9
           GSK SN N
Sbjct: 667 GSKKSNKN 674
>NP_006717
          Length = 2863

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>NP_001093591
          Length = 765

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKSSNAN 9
           SKSSN+N
Sbjct: 241 SKSSNSN 247
>NP_001093590
          Length = 811

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKSSNAN 9
           SKSSN+N
Sbjct: 287 SKSSNSN 293
>NP_001186211
          Length = 2851

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>XP_005263433
          Length = 2832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1733 GSRSSNA 1739
>XP_005263432
          Length = 2852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>XP_005263431
          Length = 2857

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>XP_006714492
          Length = 2867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>XP_005263430
          Length = 2868

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>XP_005263429
          Length = 2868

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GSKSSNA 8
            GS+SSNA
Sbjct: 1769 GSRSSNA 1775
>NP_001032401
          Length = 736

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKSSNAN 9
           SKSSN+N
Sbjct: 212 SKSSNSN 218
>NP_055508
          Length = 883

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKSSNAN 9
           SKSSN+N
Sbjct: 359 SKSSNSN 365
>NP_954592
          Length = 608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GSKSSN 7
           GSKSSN
Sbjct: 460 GSKSSN 465
>NP_001229265
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   IGSKSSNAN 9
           IGS  SN N
Sbjct: 343 IGSAESNCN 351
>NP_001128509
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   IGSKSSNAN 9
           IGS  SN N
Sbjct: 197 IGSAESNCN 205
>XP_005259765
          Length = 585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GSKSSN 7
           GSKSSN
Sbjct: 460 GSKSSN 465
>XP_006714485
          Length = 1585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IGSKSSNAN 9
            +GS++SN N
Sbjct: 1196 LGSENSNKN 1204
>XP_005263416
          Length = 1693

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IGSKSSNAN 9
            +GS++SN N
Sbjct: 1304 LGSENSNKN 1312
>XP_006714483
          Length = 1695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IGSKSSNAN 9
            +GS++SN N
Sbjct: 1306 LGSENSNKN 1314
>XP_005263415
          Length = 1701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    IGSKSSNAN 9
            +GS++SN N
Sbjct: 1312 LGSENSNKN 1320
>NP_005918
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   IGSKSSNAN 9
           IGS  SN N
Sbjct: 343 IGSAESNCN 351
>NP_005378
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 675 GNKHSNSN 682
>NP_624357
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 658 GNKHSNSN 665
>NP_624358
          Length = 1726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 658 GNKHSNSN 665
>NP_057404
          Length = 1800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 658 GNKHSNSN 665
>NP_003881
          Length = 5405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    GSKSSNAN 9
            GS++ NAN
Sbjct: 1406 GSQAGNAN 1413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    GSKSSNAN 9
            GS++ NAN
Sbjct: 2607 GSQAGNAN 2614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    GSKSSNAN 9
            GS++ NAN
Sbjct: 3808 GSQAGNAN 3815
>NP_056465
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGSKSSNAN 9
           IG+K +N N
Sbjct: 386 IGTKEANIN 394
>NP_149973
          Length = 498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKSSNAN 9
           SKSSN N
Sbjct: 155 SKSSNLN 161
>NP_001070817
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKSSNAN 9
           SKSSN N
Sbjct: 123 SKSSNLN 129
>NP_071423
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           GS+S N+N
Sbjct: 485 GSESENSN 492
>NP_004897
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSKSSNAN 9
           G+KSSN++
Sbjct: 310 GNKSSNSS 317
>NP_001257460
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSKSSNAN 9
           G+KSSN++
Sbjct: 310 GNKSSNSS 317
>NP_149971
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   IGSKSSN 7
           +GSK+SN
Sbjct: 150 LGSKASN 156
>XP_006723857
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  IGSKSSN 7
          +GSKS+N
Sbjct: 8  LGSKSAN 14
>XP_005260203
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  SKSSNAN 9
          SKSSN N
Sbjct: 79 SKSSNLN 85
>XP_006718305
          Length = 1743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 675 GNKHSNSN 682
>XP_006718304
          Length = 1753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 611 GNKHSNSN 618
>XP_006718303
          Length = 1792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 650 GNKHSNSN 657
>XP_005253007
          Length = 1817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           G+K SN+N
Sbjct: 675 GNKHSNSN 682
>XP_006714730
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           GS+S N+N
Sbjct: 112 GSESENSN 119
>XP_005272100
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSKSSNAN 9
           GS+S N+N
Sbjct: 457 GSESENSN 464
>XP_006712476
          Length = 667

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGSKSSNAN 9
           IG+K +N N
Sbjct: 362 IGTKEANIN 370
>XP_006712475
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   IGSKSSNAN 9
           IG+K +N N
Sbjct: 386 IGTKEANIN 394
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.282    0.102    0.240 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 416,719
Number of extensions: 671
Number of successful extensions: 56
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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