Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              19     869
XP_006718907                                                           19     869
XP_006718906                                                           19     869
XP_005271619                                                           19     869
XP_005271618                                                           19     869
NP_003016                                                              17    3301
NP_001186872                                                           17    3301
XP_005251606                                                           17    3301
NP_003017                                                              17    3301
NP_060762                                                              17    4312
NP_003706                                                              17    4312
NP_001276978                                                           17    4312
XP_006714460                                                           17    4312
XP_006714459                                                           17    4312
NP_060710                                                              16    5631
NP_001273278                                                           16    5631
NP_001138600                                                           16    5631
NP_001277251                                                           16    5631
NP_005500                                                              16    5631
NP_001277250                                                           16    5631
NP_872312                                                              16    5631
XP_006715439                                                           16    5631
XP_005271970                                                           16    5631
XP_005271969                                                           16    5631
XP_005271967                                                           16    5631
XP_005271966                                                           16    5631
XP_006711493                                                           16    5631
XP_005245363                                                           16    5631
XP_005245362                                                           16    5631
NP_001273191                                                           16    7355
NP_689985                                                              16    7355
NP_001123492                                                           16    7355
NP_001123493                                                           16    7355
NP_060024                                                              16    7355
NP_005168                                                              16    7355
XP_005257520                                                           16    7355
XP_005257518                                                           16    7355
XP_005257516                                                           16    7355
XP_005268826                                                           16    7355
XP_006719386                                                           16    7355
XP_005268825                                                           16    7355
XP_005268823                                                           16    7355
XP_005268824                                                           16    7355
XP_005247602                                                           16    7355
NP_001275993                                                           15    9605
NP_000225                                                              15    9605
NP_001275992                                                           15    9605
NP_060855                                                              15    9605
NP_061141                                                              15    9605
NP_665879                                                              15    9605
XP_006723280                                                           15    9605
XP_006723279                                                           15    9605
XP_005258991                                                           15    9605
XP_006723278                                                           15    9605
XP_005255020                                                           15    9605
XP_005265476                                                           15    9605
NP_057200                                                              15    9605
NP_003232                                                              15    9605
NP_001034764                                                           15    9605
NP_612151                                                              15    9605
>NP_000042
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           IAVKEN IE
Sbjct: 339 IAVKENLIE 347
>XP_006718907
          Length = 3050

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           IAVKEN IE
Sbjct: 339 IAVKENLIE 347
>XP_006718906
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           IAVKEN IE
Sbjct: 339 IAVKENLIE 347
>XP_005271619
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           IAVKEN IE
Sbjct: 339 IAVKENLIE 347
>XP_005271618
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           IAVKEN IE
Sbjct: 339 IAVKENLIE 347
>NP_003016
          Length = 368

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           I VK+NFI+
Sbjct: 133 IEVKQNFID 141
>NP_001186872
          Length = 320

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  IAVKENFIE 9
          I VK+NFI+
Sbjct: 85 IEVKQNFID 93
>XP_005251606
          Length = 340

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           I VK+NFI+
Sbjct: 121 IEVKQNFID 129
>NP_003017
          Length = 352

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   IAVKENFIE 9
           I VK+NFI+
Sbjct: 133 IEVKQNFID 141
>NP_060762
          Length = 528

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  IAVKENF 7
          IAVK+NF
Sbjct: 73 IAVKDNF 79
>NP_003706
          Length = 962

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KENFIE 9
           KENFIE
Sbjct: 574 KENFIE 579
>NP_001276978
          Length = 973

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KENFIE 9
           KENFIE
Sbjct: 585 KENFIE 590
>XP_006714460
          Length = 966

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KENFIE 9
           KENFIE
Sbjct: 578 KENFIE 583
>XP_006714459
          Length = 969

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KENFIE 9
           KENFIE
Sbjct: 581 KENFIE 586
>NP_060710
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A KENF+E
Sbjct: 427 AWKENFLE 434
>NP_001273278
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A KENF+E
Sbjct: 327 AWKENFLE 334
>NP_001138600
          Length = 1911

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IAVKENFI 8
           + +KENFI
Sbjct: 938 VVLKENFI 945
>NP_001277251
          Length = 2854

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2245 LAVKEKFI 2252
>NP_005500
          Length = 3027

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2418 LAVKEKFI 2425
>NP_001277250
          Length = 3048

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2418 LAVKEKFI 2425
>NP_872312
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           VKEN++E
Sbjct: 253 VKENYLE 259
>XP_006715439
          Length = 1911

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IAVKENFI 8
           + +KENFI
Sbjct: 938 VVLKENFI 945
>XP_005271970
          Length = 2931

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2456 LAVKEKFI 2463
>XP_005271969
          Length = 3020

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2390 LAVKEKFI 2397
>XP_005271967
          Length = 3065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2456 LAVKEKFI 2463
>XP_005271966
          Length = 3086

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    IAVKENFI 8
            +AVKE FI
Sbjct: 2456 LAVKEKFI 2463
>XP_006711493
          Length = 673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A KENF+E
Sbjct: 343 AWKENFLE 350
>XP_005245363
          Length = 723

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A KENF+E
Sbjct: 393 AWKENFLE 400
>XP_005245362
          Length = 773

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A KENF+E
Sbjct: 443 AWKENFLE 450
>NP_001273191
          Length = 266

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 81 IAVNENF 87
>NP_689985
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 81 IAVNENF 87
>NP_001123492
          Length = 838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A+K NF+E
Sbjct: 117 ALKRNFLE 124
>NP_001123493
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A+K NF+E
Sbjct: 117 ALKRNFLE 124
>NP_060024
          Length = 1801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   AVKENF 7
           A+KENF
Sbjct: 940 AIKENF 945
>NP_005168
          Length = 831

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A+K NF+E
Sbjct: 117 ALKRNFLE 124
>XP_005257520
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A+K NF+E
Sbjct: 117 ALKRNFLE 124
>XP_005257518
          Length = 790

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A+K NF+E
Sbjct: 117 ALKRNFLE 124
>XP_005257516
          Length = 844

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A+K NF+E
Sbjct: 117 ALKRNFLE 124
>XP_005268826
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 16 IAVNENF 22
>XP_006719386
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 81 IAVNENF 87
>XP_005268825
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 81 IAVNENF 87
>XP_005268823
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 81 IAVNENF 87
>XP_005268824
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          IAV ENF
Sbjct: 81 IAVNENF 87
>XP_005247602
          Length = 1665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   AVKENF 7
           A+KENF
Sbjct: 804 AIKENF 809
>NP_001275993
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 614 LRENFVE 620
>NP_000225
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 682 LRENFVE 688
>NP_001275992
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 651 LRENFVE 657
>NP_060855
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AVKENFIE 9
           A K+NF+E
Sbjct: 131 AEKDNFLE 138
>NP_061141
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VKENFIE 9
           VKE F+E
Sbjct: 560 VKEEFVE 566
>NP_665879
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  VKENFIE 9
          VK NF+E
Sbjct: 17 VKRNFLE 23
>XP_006723280
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 276 LRENFVE 282
>XP_006723279
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 682 LRENFVE 688
>XP_005258991
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 652 LRENFVE 658
>XP_006723278
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VKENFIE 9
           ++ENF+E
Sbjct: 681 LRENFVE 687
>XP_005255020
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VKENFIE 9
           VKE F+E
Sbjct: 415 VKEEFVE 421
>XP_005265476
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VKENFI 8
           VKENF+
Sbjct: 385 VKENFL 390
>NP_057200
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  VKENFIE 9
          VK NF+E
Sbjct: 17 VKRNFLE 23
>NP_003232
          Length = 684

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VKENFI 8
           VKENF+
Sbjct: 468 VKENFL 473
>NP_001034764
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VKENFIE 9
           VKE F+E
Sbjct: 545 VKEEFVE 551
>NP_612151
          Length = 126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  IAVKENF 7
          +AVK NF
Sbjct: 34 VAVKNNF 40
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.140    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 502,043
Number of extensions: 611
Number of successful extensions: 60
Number of sequences better than 10000.0: 60
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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