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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     390
NP_000170                                                              20     390
NP_001268422                                                           20     390
NP_001268423                                                           20     390
XP_005264328                                                           20     390
NP_002449                                                              16    5631
NP_008915                                                              16    5631
NP_005594                                                              16    7355
NP_001018066                                                           16    7355
NP_003374                                                              16    7355
XP_006722545                                                           16    7355
XP_006722544                                                           16    7355
XP_006716928                                                           16    7355
XP_006716927                                                           16    7355
NP_001007256                                                           16    7355
NP_001185480                                                           15    9605
NP_077742                                                              15    9605
NP_077744                                                              15    9605
NP_001185481                                                           15    9605
NP_000369                                                              15    9605
NP_001177874                                                           15    9605
NP_733828                                                              15    9605
NP_001177876                                                           15    9605
NP_005649                                                              15    9605
NP_062547                                                              15    9605
NP_851782                                                              15    9605
XP_006720918                                                           15    9605
XP_005262782                                                           15    9605
XP_006714141                                                           15    9605
XP_005262780                                                           15    9605
XP_005262779                                                           15    9605
NP_001397                                                              15    9605

>NP_001268421
          Length = 1230

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ATFDGTAI 9
            ATFDGTAI
Sbjct: 1090 ATFDGTAI 1097



>NP_000170
          Length = 1360

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ATFDGTAI 9
            ATFDGTAI
Sbjct: 1220 ATFDGTAI 1227



>NP_001268422
          Length = 1058

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   ATFDGTAI 9
           ATFDGTAI
Sbjct: 918 ATFDGTAI 925



>NP_001268423
          Length = 1058

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   ATFDGTAI 9
           ATFDGTAI
Sbjct: 918 ATFDGTAI 925



>XP_005264328
          Length = 1261

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ATFDGTAI 9
            ATFDGTAI
Sbjct: 1121 ATFDGTAI 1128



>NP_002449
          Length = 5762

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    ATFDGTA 8
            +TFDGT+
Sbjct: 5084 STFDGTS 5090



>NP_008915
          Length = 228

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IATFDGTAI 9
          ++T DGT I
Sbjct: 32 VSTIDGTVI 40



>NP_005594
          Length = 1251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   IATFDG 6
           +ATFDG
Sbjct: 256 LATFDG 261



>NP_001018066
          Length = 845

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IATFDGT 7
           +AT DGT
Sbjct: 539 VATLDGT 545



>NP_003374
          Length = 873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IATFDGT 7
           +AT DGT
Sbjct: 539 VATLDGT 545



>XP_006722545
          Length = 982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   IATFDG 6
           +ATFDG
Sbjct: 256 LATFDG 261



>XP_006722544
          Length = 1251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   IATFDG 6
           +ATFDG
Sbjct: 256 LATFDG 261



>XP_006716928
          Length = 850

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IATFDGT 7
           +AT DGT
Sbjct: 544 VATLDGT 550



>XP_006716927
          Length = 878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IATFDGT 7
           +AT DGT
Sbjct: 544 VATLDGT 550



>NP_001007256
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ATFDGTA 8
           ++FDGTA
Sbjct: 242 SSFDGTA 248



>NP_001185480
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3  TFDGT 7
          TFDGT
Sbjct: 9  TFDGT 13



>NP_077742
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   TFDGT 7
           TFDGT
Sbjct: 221 TFDGT 225



>NP_077744
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   TFDGT 7
           TFDGT
Sbjct: 221 TFDGT 225



>NP_001185481
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3  TFDGT 7
          TFDGT
Sbjct: 9  TFDGT 13



>NP_000369
          Length = 497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   TFDGT 7
           TFDGT
Sbjct: 221 TFDGT 225



>NP_001177874
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 263 ASFDGT 268



>NP_733828
          Length = 1322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 412 ASFDGT 417



>NP_001177876
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 141 ASFDGT 146



>NP_005649
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 263 ASFDGT 268



>NP_062547
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   TFDGT 7
           TFDGT
Sbjct: 457 TFDGT 461



>NP_851782
          Length = 1283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 388 ASFDGT 393



>XP_006720918
          Length = 175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IATFDGTAI 9
           ++ +DGTAI
Sbjct: 103 VSHWDGTAI 111



>XP_005262782
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 314 ASFDGT 319



>XP_006714141
          Length = 1259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 412 ASFDGT 417



>XP_005262780
          Length = 1307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 412 ASFDGT 417



>XP_005262779
          Length = 1314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ATFDGT 7
           A+FDGT
Sbjct: 412 ASFDGT 417



>NP_001397
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   IATFDGT 7
           IAT DGT
Sbjct: 138 IATSDGT 144



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.140    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 600,245
Number of extensions: 830
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)