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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     509
XP_005251512                                                           20     509
NP_945148                                                              17    3301
NP_689714                                                              17    3301
NP_945147                                                              17    3301
NP_945146                                                              17    3301
XP_006711254                                                           17    3301
NP_079350                                                              15    9605
NP_066969                                                              15    9605
NP_958934                                                              15    9605
XP_006714380                                                           15    9605
XP_006714379                                                           15    9605
XP_006714378                                                           15    9605
XP_006714377                                                           15    9605
NP_005536                                                              15    9605

>NP_004963
          Length = 1132

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  HNMSALMSE 9
          HNM ALMSE
Sbjct: 82 HNMFALMSE 90



>XP_005251512
          Length = 1132

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  HNMSALMSE 9
          HNM ALMSE
Sbjct: 82 HNMFALMSE 90



>NP_945148
          Length = 452

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NMSALMSE 9
           NMS LMSE
Sbjct: 375 NMSKLMSE 382



>NP_689714
          Length = 492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NMSALMSE 9
           NMS LMSE
Sbjct: 384 NMSKLMSE 391



>NP_945147
          Length = 461

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NMSALMSE 9
           NMS LMSE
Sbjct: 384 NMSKLMSE 391



>NP_945146
          Length = 483

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NMSALMSE 9
           NMS LMSE
Sbjct: 375 NMSKLMSE 382



>XP_006711254
          Length = 492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   NMSALMSE 9
           NMS LMSE
Sbjct: 384 NMSKLMSE 391



>NP_079350
          Length = 4012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HNMSALMSE 9
            HN+  L+SE
Sbjct: 3361 HNLHFLLSE 3369



>NP_066969
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  NMSALMSE 9
          N+S+L+SE
Sbjct: 58 NLSSLLSE 65



>NP_958934
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HNMSAL 6
           HN+SAL
Sbjct: 119 HNLSAL 124



>XP_006714380
          Length = 3897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HNMSALMSE 9
            HN+  L+SE
Sbjct: 3246 HNLHFLLSE 3254



>XP_006714379
          Length = 3936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HNMSALMSE 9
            HN+  L+SE
Sbjct: 3285 HNLHFLLSE 3293



>XP_006714378
          Length = 3965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HNMSALMSE 9
            HN+  L+SE
Sbjct: 3314 HNLHFLLSE 3322



>XP_006714377
          Length = 4010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    HNMSALMSE 9
            HN+  L+SE
Sbjct: 3359 HNLHFLLSE 3367



>NP_005536
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   HNMSAL 6
           HN+SAL
Sbjct: 119 HNLSAL 124



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.107    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 237,235
Number of extensions: 260
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)