Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              20     299
NP_055796                                                              17    2528
NP_001155130                                                           17    4312
NP_001155128                                                           17    4312
NP_001123645                                                           17    4312
NP_001185471                                                           16    5631
NP_001185472                                                           16    5631
NP_060028                                                              16    5631
NP_001257903                                                           16    5631
NP_079225                                                              16    5631
NP_001029345                                                           16    5631
XP_006721269                                                           16    5631
XP_006714376                                                           16    5631
XP_006714375                                                           16    5631
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
NP_001254479                                                           16    7355
NP_001020605                                                           15    9605
NP_001020604                                                           15    9605
NP_000894                                                              15    9605
NP_001273066                                                           15    9605
NP_001257293                                                           15    9605
NP_001535                                                              15    9605
NP_036322                                                              15    9605
NP_001165102                                                           15    9605
NP_001257294                                                           15    9605
NP_443178                                                              15    9605
NP_001165103                                                           15    9605
NP_071772                                                              15    9605
NP_001034813                                                           15    9605
XP_006713545                                                           15    9605
XP_006713544                                                           15    9605
XP_005246327                                                           15    9605
XP_006712296                                                           15    9605
NP_777638                                                              15    9605
NP_001034221                                                           15    9605
>NP_005350
          Length = 552

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   HIGKSVQLE 9
           HIGK VQLE
Sbjct: 382 HIGKGVQLE 390
>NP_055796
          Length = 1946

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   HIGKSVQLE 9
           H GKSV L+
Sbjct: 568 HFGKSVHLQ 576
>NP_001155130
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HIGKSVQLE 9
           H GK++QL+
Sbjct: 410 HTGKALQLQ 418
>NP_001155128
          Length = 758

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HIGKSVQLE 9
           H GK++QL+
Sbjct: 423 HTGKALQLQ 431
>NP_001123645
          Length = 761

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HIGKSVQLE 9
           H GK++QL+
Sbjct: 426 HTGKALQLQ 434
>NP_001185471
          Length = 950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   HIGKSVQLE 9
           ++G+S+QLE
Sbjct: 278 NVGESMQLE 286
>NP_001185472
          Length = 940

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   HIGKSVQLE 9
           ++G+S+QLE
Sbjct: 268 NVGESMQLE 276
>NP_060028
          Length = 1017

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   HIGKSVQLE 9
           ++G+S+QLE
Sbjct: 251 NVGESMQLE 259
>NP_001257903
          Length = 5121

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   HIGKSVQLE 9
           ++G+S+QLE
Sbjct: 251 NVGESMQLE 259
>NP_079225
          Length = 1192

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HIGKSVQLE 9
          HI K+ Q+E
Sbjct: 78 HINKTTQIE 86
>NP_001029345
          Length = 923

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           HIG SVQ
Sbjct: 543 HIGMSVQ 549
>XP_006721269
          Length = 5138

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   HIGKSVQLE 9
           ++G+S+QLE
Sbjct: 268 NVGESMQLE 276
>XP_006714376
          Length = 815

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HIGKSVQLE 9
          HI K+ Q+E
Sbjct: 78 HINKTTQIE 86
>XP_006714375
          Length = 1208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HIGKSVQLE 9
          HI K+ Q+E
Sbjct: 78 HINKTTQIE 86
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IGKSVQLE 9
            +GK VQL+
Sbjct: 8432 VGKEVQLQ 8439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GKSVQLE 9
            G+S+QLE
Sbjct: 7306 GESIQLE 7312
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IGKSVQLE 9
            +GK VQL+
Sbjct: 7505 VGKEVQLQ 7512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GKSVQLE 9
            G+S+QLE
Sbjct: 6379 GESIQLE 6385
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IGKSVQLE 9
            +GK VQL+
Sbjct: 8433 VGKEVQLQ 8440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GKSVQLE 9
            G+S+QLE
Sbjct: 7307 GESIQLE 7313
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IGKSVQLE 9
            +GK VQL+
Sbjct: 8433 VGKEVQLQ 8440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GKSVQLE 9
            G+S+QLE
Sbjct: 7307 GESIQLE 7313
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IGKSVQLE 9
            +GK VQL+
Sbjct: 8749 VGKEVQLQ 8756

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GKSVQLE 9
            G+S+QLE
Sbjct: 7623 GESIQLE 7629
>NP_001020605
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HIGKSV 6
           H+GKS+
Sbjct: 221 HLGKSI 226
>NP_001020604
          Length = 240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HIGKSV 6
           H+GKS+
Sbjct: 225 HLGKSI 230
>NP_000894
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HIGKSV 6
           H+GKS+
Sbjct: 259 HLGKSI 264
>NP_001273066
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   HIGKSV 6
           H+GKS+
Sbjct: 187 HLGKSI 192
>NP_001257293
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           H+G S+Q
Sbjct: 532 HVGMSIQ 538
>NP_001535
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  GKSVQL 8
          GKSVQL
Sbjct: 62 GKSVQL 67
>NP_036322
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           H+G S+Q
Sbjct: 522 HVGMSIQ 528
>NP_001165102
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  IGKSVQLE 9
          +GK+++LE
Sbjct: 89 LGKAIELE 96
>NP_001257294
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           H+G S+Q
Sbjct: 421 HVGMSIQ 427
>NP_443178
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  IGKSVQLE 9
          +GK+++LE
Sbjct: 11 LGKAIELE 18
>NP_001165103
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  IGKSVQLE 9
          +GK+++LE
Sbjct: 61 LGKAIELE 68
>NP_071772
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  GKSVQL 8
          GKSVQL
Sbjct: 62 GKSVQL 67
>NP_001034813
          Length = 506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  IGKSVQLE 9
          +GK+++LE
Sbjct: 89 LGKAIELE 96
>XP_006713545
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           H+G S+Q
Sbjct: 520 HVGMSIQ 526
>XP_006713544
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           H+G S+Q
Sbjct: 522 HVGMSIQ 528
>XP_005246327
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  IGKSVQLE 9
          +GK+++LE
Sbjct: 89 LGKAIELE 96
>XP_006712296
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  IGKSVQLE 9
          +GK+++LE
Sbjct: 89 LGKAIELE 96
>NP_777638
          Length = 587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   HIGKSVQ 7
           H GKS++
Sbjct: 399 HFGKSIE 405
>NP_001034221
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  GKSVQL 8
          GKSVQL
Sbjct: 62 GKSVQL 67
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.132    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 476,651
Number of extensions: 525
Number of successful extensions: 43
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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