Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_075391                                                              17    2528
XP_006726267                                                           17    2528
XP_005276792                                                           17    2528
XP_006711553                                                           17    2528
XP_005245516                                                           17    2528
NP_065914                                                              16    5631
NP_001167628                                                           16    5631
NP_001276323                                                           16    5631
NP_001025110                                                           16    5631
NP_001161055                                                           16    5631
NP_065961                                                              16    5631
NP_001158137                                                           16    5631
XP_006716674                                                           16    5631
XP_006716134                                                           16    5631
XP_005245596                                                           16    5631
NP_003559                                                              16    5631
NP_001167629                                                           16    5631
NP_758952                                                              16    7355
NP_000200                                                              15    9605
NP_003097                                                              15    9605
NP_001166938                                                           15    9605
NP_003301                                                              15    9605
NP_690599                                                              15    9605
XP_006711956                                                           15    9605
>NP_075391
          Length = 1042

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HHGAAQI 7
           HHG AQ+
Sbjct: 219 HHGTAQL 225
>XP_006726267
          Length = 1053

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HHGAAQI 7
           HHG AQ+
Sbjct: 164 HHGTAQL 170
>XP_005276792
          Length = 1108

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HHGAAQI 7
           HHG AQ+
Sbjct: 219 HHGTAQL 225
>XP_006711553
          Length = 1053

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HHGAAQI 7
           HHG AQ+
Sbjct: 164 HHGTAQL 170
>XP_005245516
          Length = 1108

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   HHGAAQI 7
           HHG AQ+
Sbjct: 219 HHGTAQL 225
>NP_065914
          Length = 1243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 481 HGAAQEAL 488
>NP_001167628
          Length = 1181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 419 HGAAQEAL 426
>NP_001276323
          Length = 1231

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 469 HGAAQEAL 476
>NP_001025110
          Length = 1231

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 469 HGAAQEAL 476
>NP_001161055
          Length = 1231

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 469 HGAAQEAL 476
>NP_065961
          Length = 1934

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  HGAAQIAM 9
          H AAQ+A+
Sbjct: 87 HSAAQVAL 94
>NP_001158137
          Length = 1950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  HGAAQIAM 9
          H AAQ+A+
Sbjct: 87 HSAAQVAL 94
>XP_006716674
          Length = 845

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 481 HGAAQEAL 488
>XP_006716134
          Length = 1847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  HGAAQIAM 9
          H AAQ+A+
Sbjct: 11 HSAAQVAL 18
>XP_005245596
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HG AQ+A+
Sbjct: 254 HGDAQVAL 261
>NP_003559
          Length = 345

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HG AQ+A+
Sbjct: 254 HGDAQVAL 261
>NP_001167629
          Length = 1145

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HGAAQIAM 9
           HGAAQ A+
Sbjct: 469 HGAAQEAL 476
>NP_758952
          Length = 1137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   HHGAAQ 6
           HH AAQ
Sbjct: 947 HHSAAQ 952
>NP_000200
          Length = 283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  HHGAAQIAM 9
          HH  AQ+A+
Sbjct: 84 HHLPAQLAL 92
>NP_003097
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HGAAQI 7
           HGAAQ+
Sbjct: 189 HGAAQM 194
>NP_001166938
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  HHGAAQIAM 9
          H+G++ +AM
Sbjct: 11 HYGSSPLAM 19
>NP_003301
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 1   HHGAAQIA 8
           HH   Q+A
Sbjct: 195 HHNCTQVA 202
>NP_690599
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  HHGAAQIAM 9
          H+G++ +AM
Sbjct: 11 HYGSSPLAM 19
>XP_006711956
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 1   HHGAAQIA 8
           HH   Q+A
Sbjct: 95  HHNCTQVA 102
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.124    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 438,538
Number of extensions: 234
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package