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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005245596                                                           17    4312
NP_003559                                                              17    4312
NP_065961                                                              16    5631
NP_001158137                                                           16    5631
XP_006716134                                                           16    5631
NP_005268                                                              16    7355
NP_963885                                                              16    7355
NP_001008                                                              16    7355
NP_001248377                                                           16    7355
NP_001248376                                                           16    7355
NP_963886                                                              16    7355
NP_112236                                                              16    7355
XP_006714246                                                           16    7355

>XP_005245596
          Length = 362

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HGAAQIAMV 9
           HG AQ+A++
Sbjct: 254 HGDAQVALL 262



>NP_003559
          Length = 345

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   HGAAQIAMV 9
           HG AQ+A++
Sbjct: 254 HGDAQVALL 262



>NP_065961
          Length = 1934

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HGAAQIAM 8
          H AAQ+A+
Sbjct: 87 HSAAQVAL 94



>NP_001158137
          Length = 1950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HGAAQIAM 8
          H AAQ+A+
Sbjct: 87 HSAAQVAL 94



>XP_006716134
          Length = 1847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  HGAAQIAM 8
          H AAQ+A+
Sbjct: 11 HSAAQVAL 18



>NP_005268
          Length = 278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAA IAMV
Sbjct: 222 GAAVIAMV 229



>NP_963885
          Length = 278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAA IAMV
Sbjct: 222 GAAVIAMV 229



>NP_001008
          Length = 151

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  HGAAQIAMV 9
          HG AQ+  V
Sbjct: 58 HGVAQVRFV 66



>NP_001248377
          Length = 271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAA IAMV
Sbjct: 215 GAAVIAMV 222



>NP_001248376
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAA IAMV
Sbjct: 187 GAAVIAMV 194



>NP_963886
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAA IAMV
Sbjct: 211 GAAVIAMV 218



>NP_112236
          Length = 387

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAAQ+A V
Sbjct: 190 GAAQVAAV 197



>XP_006714246
          Length = 215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GAAQIAMV 9
           GAA IAMV
Sbjct: 159 GAAVIAMV 166



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.127    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 244,836
Number of extensions: 144
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)