Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245202                                                           19     665
NP_001245200                                                           19     665
NP_001161091                                                           19     665
NP_000240                                                              19     665
NP_001161090                                                           19     665
NP_001161089                                                           19     665
NP_001245203                                                           19     665
XP_005265223                                                           19     665
XP_005265221                                                           19     665
XP_005265220                                                           19     665
XP_005265218                                                           19     665
NP_001273135                                                           17    2528
NP_055710                                                              17    2528
XP_006715443                                                           17    2528
XP_005248732                                                           17    2528
XP_005248731                                                           17    2528
XP_005248231                                                           16    5631
NP_060793                                                              16    5631
NP_001074008                                                           16    7355
NP_006355                                                              16    7355
XP_005267319                                                           16    7355
NP_001180360                                                           15    9605
NP_057051                                                              15    9605
NP_036543                                                              15    9605
NP_001005376                                                           15    9605
NP_001005377                                                           15    9605
NP_002650                                                              15    9605
NP_006368                                                              15    9605
NP_006460                                                              15    9605
NP_001269266                                                           15    9605
NP_001269267                                                           15    9605
NP_077295                                                              15    9605
NP_001074003                                                           15    9605
NP_001073890                                                           15    9605
NP_001165806                                                           15    9605
NP_775871                                                              15    9605
NP_001129497                                                           15    9605
NP_005023                                                              15    9605
XP_005259047                                                           15    9605
XP_005259046                                                           15    9605
XP_006723304                                                           15    9605
XP_005259045                                                           15    9605
XP_006722755                                                           15    9605
XP_005257220                                                           15    9605
XP_005254452                                                           15    9605
XP_005254451                                                           15    9605
XP_006716779                                                           15    9605
XP_005244900                                                           15    9605
XP_005270795                                                           15    9605
NP_031385                                                              15    9605
>NP_001245202
          Length = 515

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 213 GTSEMSEKR 221
>NP_001245200
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 454 GTSEMSEKR 462
>NP_001161091
          Length = 515

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 213 GTSEMSEKR 221
>NP_000240
          Length = 756

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 454 GTSEMSEKR 462
>NP_001161090
          Length = 515

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 213 GTSEMSEKR 221
>NP_001161089
          Length = 658

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 356 GTSEMSEKR 364
>NP_001245203
          Length = 515

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 213 GTSEMSEKR 221
>XP_005265223
          Length = 415

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 113 GTSEMSEKR 121
>XP_005265221
          Length = 515

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 213 GTSEMSEKR 221
>XP_005265220
          Length = 515

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 213 GTSEMSEKR 221
>XP_005265218
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GTSEMS KR
Sbjct: 385 GTSEMSEKR 393
>NP_001273135
          Length = 1327

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GTSEMSAKR 9
            G  EMSAKR
Sbjct: 1050 GREEMSAKR 1058
>NP_055710
          Length = 1403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GTSEMSAKR 9
            G  EMSAKR
Sbjct: 1126 GREEMSAKR 1134
>XP_006715443
          Length = 1327

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GTSEMSAKR 9
            G  EMSAKR
Sbjct: 1050 GREEMSAKR 1058
>XP_005248732
          Length = 1330

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GTSEMSAKR 9
            G  EMSAKR
Sbjct: 1053 GREEMSAKR 1061
>XP_005248731
          Length = 1365

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    GTSEMSAKR 9
            G  EMSAKR
Sbjct: 1088 GREEMSAKR 1096
>XP_005248231
          Length = 476

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GTSEMSA 7
          GTSEM+A
Sbjct: 93 GTSEMNA 99
>NP_060793
          Length = 481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GTSEMSA 7
          GTSEM+A
Sbjct: 93 GTSEMNA 99
>NP_001074008
          Length = 1101

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SEMSAKR 9
           SE+SAKR
Sbjct: 604 SELSAKR 610
>NP_006355
          Length = 765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GT ++SAK+
Sbjct: 191 GTKDLSAKQ 199
>XP_005267319
          Length = 789

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           GT ++SAK+
Sbjct: 191 GTKDLSAKQ 199
>NP_001180360
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSEMSAK 8
           TSE+SAK
Sbjct: 358 TSEISAK 364
>NP_057051
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSEMSAK 8
           TSE+SAK
Sbjct: 358 TSEISAK 364
>NP_036543
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSEMSAK 8
           TSE+SAK
Sbjct: 303 TSEISAK 309
>NP_001005376
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 121 GSSDMSCER 129
>NP_001005377
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 121 GSSDMSCER 129
>NP_002650
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 121 GSSDMSCER 129
>NP_006368
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTSEMSAK 8
           GTS+ SAK
Sbjct: 594 GTSKWSAK 601
>NP_006460
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GTSEMSAK 8
           GT+EM+ K
Sbjct: 362 GTAEMNGK 369
>NP_001269266
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  GTSEMSAK 8
          GTSE+S++
Sbjct: 86 GTSELSSE 93
>NP_001269267
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  GTSEMSAK 8
          GTSE+S++
Sbjct: 63 GTSELSSE 70
>NP_077295
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTSEMSAKR 9
           GTS  SA+R
Sbjct: 159 GTSAASARR 167
>NP_001074003
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GTSEMSAK 8
            GTS+ SAK
Sbjct: 1214 GTSKWSAK 1221
>NP_001073890
          Length = 1703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTSEMSAK 8
           GTS+ SAK
Sbjct: 670 GTSKWSAK 677
>NP_001165806
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  GTSEMSAK 8
          GTSE+S++
Sbjct: 81 GTSELSSE 88
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    GTSEMSAK 8
            GT+E+SA+
Sbjct: 5431 GTTELSAE 5438
>NP_001129497
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GTSEMSAK 8
           GTSE+S++
Sbjct: 108 GTSELSSE 115
>NP_005023
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   GTSEMSAK 8
           GTSE+S++
Sbjct: 108 GTSELSSE 115
>XP_005259047
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 121 GSSDMSCER 129
>XP_005259046
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 121 GSSDMSCER 129
>XP_006723304
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 96  GSSDMSCER 104
>XP_005259045
          Length = 330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTSEMSAKR 9
           G+S+MS +R
Sbjct: 116 GSSDMSCER 124
>XP_006722755
          Length = 1722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTSEMSAK 8
           GTS+ SAK
Sbjct: 670 GTSKWSAK 677
>XP_005257220
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GTSEMSAKR 9
           GT  MSA R
Sbjct: 303 GTGRMSAPR 311
>XP_005254452
          Length = 2188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GTSEMSAK 8
            GTS+ SAK
Sbjct: 1214 GTSKWSAK 1221
>XP_005254451
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GTSEMSAK 8
            GTS+ SAK
Sbjct: 1214 GTSKWSAK 1221
>XP_006716779
          Length = 2399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    GTSEMSAK 8
            GTS+ SAK
Sbjct: 1402 GTSKWSAK 1409
>XP_005244900
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GTSEMSAK 8
           GT+EM+ K
Sbjct: 144 GTAEMNGK 151
>XP_005270795
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSEMSAK 8
           TSE+SAK
Sbjct: 334 TSEISAK 340
>NP_031385
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   GTSEMSAKR 9
           GT  MSA R
Sbjct: 303 GTGRMSAPR 311
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.104    0.245 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,118
Number of extensions: 475
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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