Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001119588                                                           18    1135
NP_001119587                                                           18    1135
NP_001119589                                                           18    1135
NP_660350                                                              17    2528
XP_006717758                                                           17    2528
NP_059138                                                              17    3301
NP_001139281                                                           17    3301
NP_001070654                                                           17    3301
NP_079005                                                              17    3301
XP_006723449                                                           17    3301
NP_003142                                                              16    5631
NP_001230764                                                           16    5631
NP_001013680                                                           16    5631
XP_006712782                                                           16    5631
XP_005246874                                                           16    5631
NP_612486                                                              16    7355
XP_005254235                                                           16    7355
XP_005254233                                                           16    7355
XP_005254234                                                           16    7355
XP_006714302                                                           16    7355
XP_005263127                                                           16    7355
NP_061908                                                              16    7355
NP_001245258                                                           15    9605
NP_001245260                                                           15    9605
NP_001245259                                                           15    9605
NP_818932                                                              15    9605
NP_036288                                                              15    9605
XP_005260382                                                           15    9605
XP_005260381                                                           15    9605
XP_006723804                                                           15    9605
XP_005260380                                                           15    9605
XP_006723803                                                           15    9605
XP_005260379                                                           15    9605
XP_005260378                                                           15    9605
XP_005260377                                                           15    9605
XP_006723802                                                           15    9605
XP_005260376                                                           15    9605
XP_006723801                                                           15    9605
XP_006723800                                                           15    9605
XP_006723799                                                           15    9605
XP_006723798                                                           15    9605
XP_005251493                                                           15    9605
>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 154 GTRVRAMAI 162
>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 115 GTRVRAMAI 123
>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 154 GTRVRAMAI 162
>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 154 GTRVRAMAI 162
>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 115 GTRVRAMAI 123
>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 115 GTRVRAMAI 123
>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 115 GTRVRAMAI 123
>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 154 GTRVRAMAI 162
>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTRVRAM I
Sbjct: 115 GTRVRAMAI 123
>NP_001119588
          Length = 209

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRVRAMDI 9
          GTRVRAM I
Sbjct: 22 GTRVRAMAI 30
>NP_001119587
          Length = 261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRVRAMDI 9
          GTRVRAM I
Sbjct: 22 GTRVRAMAI 30
>NP_001119589
          Length = 214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  GTRVRAMDI 9
          GTRVRAM I
Sbjct: 22 GTRVRAMAI 30
>NP_660350
          Length = 609

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TRVRAMD 8
           TR+RAMD
Sbjct: 342 TRIRAMD 348
>XP_006717758
          Length = 630

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TRVRAMD 8
           TR+RAMD
Sbjct: 363 TRIRAMD 369
>NP_059138
          Length = 1032

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GTRVRAMDI 9
           GTR+R +D+
Sbjct: 700 GTRLRRLDV 708
>NP_001139281
          Length = 2036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GTRVRAMD 8
            G+RVRAM+
Sbjct: 1393 GSRVRAME 1400
>NP_001070654
          Length = 2003

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GTRVRAMD 8
            G+RVRAM+
Sbjct: 1360 GSRVRAME 1367
>NP_079005
          Length = 1995

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GTRVRAMD 8
            G+RVRAM+
Sbjct: 1352 GSRVRAME 1359
>XP_006723449
          Length = 2003

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    GTRVRAMD 8
            G+RVRAM+
Sbjct: 1360 GSRVRAME 1367
>NP_003142
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVRAMDI 9
           GT V+AM I
Sbjct: 379 GTNVKAMSI 387
>NP_001230764
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVRAMDI 9
           GT V+AM I
Sbjct: 379 GTNVKAMSI 387
>NP_001013680
          Length = 473

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TRVRAMDI 9
           TR+RA+D+
Sbjct: 115 TRLRALDL 122
>XP_006712782
          Length = 748

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVRAMDI 9
           GT V+AM I
Sbjct: 379 GTNVKAMSI 387
>XP_005246874
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTRVRAMDI 9
           GT V+AM I
Sbjct: 388 GTNVKAMSI 396
>NP_612486
          Length = 1227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTRVRAM 7
           G+R+RAM
Sbjct: 482 GSRIRAM 488
>XP_005254235
          Length = 1017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTRVRAM 7
           G+R+RAM
Sbjct: 483 GSRIRAM 489
>XP_005254233
          Length = 1228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTRVRAM 7
           G+R+RAM
Sbjct: 483 GSRIRAM 489
>XP_005254234
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GTRVRAM 7
           G+R+RAM
Sbjct: 483 GSRIRAM 489
>XP_006714302
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TRVRAMD 8
           TR+RA+D
Sbjct: 601 TRIRAID 607
>XP_005263127
          Length = 1134

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TRVRAMD 8
           TR+RA+D
Sbjct: 601 TRIRAID 607
>NP_061908
          Length = 1135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TRVRAMD 8
           TR+RA+D
Sbjct: 601 TRIRAID 607
>NP_001245258
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>NP_001245260
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 645 TRVSAMD 651
>NP_001245259
          Length = 701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 539 TRVSAMD 545
>NP_818932
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 645 TRVSAMD 651
>NP_036288
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>XP_005260382
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 570 TRVSAMD 576
>XP_005260381
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 582 TRVSAMD 588
>XP_006723804
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 657 TRVSAMD 663
>XP_005260380
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 707 TRVSAMD 713
>XP_006723803
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>XP_005260379
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 706 TRVSAMD 712
>XP_005260378
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 707 TRVSAMD 713
>XP_005260377
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 707 TRVSAMD 713
>XP_006723802
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 718 TRVSAMD 724
>XP_005260376
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>XP_006723801
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>XP_006723800
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>XP_006723799
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 719 TRVSAMD 725
>XP_006723798
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TRVRAMD 8
           TRV AMD
Sbjct: 782 TRVSAMD 788
>XP_005251493
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GTRVRAMDI 9
          G+R R++DI
Sbjct: 9  GSRSRSLDI 17
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.142    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 326,618
Number of extensions: 612
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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