Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001094871                                                           17    3301
XP_006723390                                                           17    3301
NP_001004470                                                           17    4312
NP_003626                                                              17    4312
XP_005270313                                                           17    4312
XP_005270312                                                           17    4312
XP_006718109                                                           17    4312
NP_000101                                                              16    5631
NP_060787                                                              16    5631
XP_005248229                                                           16    5631
XP_006714030                                                           16    5631
XP_006710460                                                           16    5631
XP_005270619                                                           16    5631
NP_116213                                                              16    7355
NP_001004125                                                           16    7355
NP_001135918                                                           16    7355
NP_065208                                                              16    7355
NP_000669                                                              16    7355
NP_000557                                                              16    7355
NP_001004340                                                           16    7355
NP_001017986                                                           16    7355
NP_001231839                                                           16    7355
XP_005273533                                                           16    7355
XP_005263884                                                           16    7355
XP_006712870                                                           16    7355
XP_005245015                                                           16    7355
XP_005245014                                                           16    7355
NP_065210                                                              16    7355
NP_065209                                                              16    7355
NP_000028                                                              16    7355
NP_005682                                                              15    9605
NP_064693                                                              15    9605
NP_001035830                                                           15    9605
NP_689498                                                              15    9605
NP_115486                                                              15    9605
XP_005253348                                                           15    9605
XP_005253347                                                           15    9605
XP_006719088                                                           15    9605
XP_005253346                                                           15    9605
XP_005253344                                                           15    9605
XP_005253345                                                           15    9605
XP_005253343                                                           15    9605
XP_005253341                                                           15    9605
XP_005266178                                                           15    9605
XP_005266176                                                           15    9605
>NP_001094871
          Length = 348

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GTPLALR 7
          GTPLAL+
Sbjct: 85 GTPLALK 91
>XP_006723390
          Length = 348

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  GTPLALR 7
          GTPLAL+
Sbjct: 85 GTPLALK 91
>NP_001004470
          Length = 398

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GTPLALRAV 9
          GTP ALR +
Sbjct: 46 GTPAALRTL 54
>NP_003626
          Length = 883

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TPLALRAV 9
           TPLALR++
Sbjct: 739 TPLALRSL 746
>XP_005270313
          Length = 509

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TPLALRAV 9
           TPLALR++
Sbjct: 365 TPLALRSL 372
>XP_005270312
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TPLALRAV 9
           TPLALR++
Sbjct: 739 TPLALRSL 746
>XP_006718109
          Length = 821

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TPLALRAV 9
           TPLALR++
Sbjct: 677 TPLALRSL 684
>NP_000101
          Length = 1025

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+ALRAV
Sbjct: 772 PIALRAV 778
>NP_060787
          Length = 526

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPLALRA 8
           G+P ALRA
Sbjct: 251 GSPTALRA 258
>XP_005248229
          Length = 461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPLALRA 8
           G+P ALRA
Sbjct: 186 GSPTALRA 193
>XP_006714030
          Length = 495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPLALRA 8
           G+P ALRA
Sbjct: 220 GSPTALRA 227
>XP_006710460
          Length = 889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+ALRAV
Sbjct: 772 PIALRAV 778
>XP_005270619
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+ALRAV
Sbjct: 700 PIALRAV 706
>NP_116213
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TPLALRAV 9
          TP A+RAV
Sbjct: 36 TPAAVRAV 43
>NP_001004125
          Length = 212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GTPLALRA 8
           G+P ALRA
Sbjct: 152 GSPRALRA 159
>NP_001135918
          Length = 1897

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TPLALR 7
            TPLALR
Sbjct: 1421 TPLALR 1426
>NP_065208
          Length = 1856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TPLALR 7
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_000669
          Length = 572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GTPLALRAV 9
           G PLAL A+
Sbjct: 459 GAPLALTAL 467
>NP_000557
          Length = 374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GTPLALR 7
           G PLALR
Sbjct: 117 GEPLALR 123
>NP_001004340
          Length = 188

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GTPLALR 7
          G PLALR
Sbjct: 25 GEPLALR 31
>NP_001017986
          Length = 280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GTPLALR 7
           G PLALR
Sbjct: 117 GEPLALR 123
>NP_001231839
          Length = 224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GTPLALR 7
           G PLALR
Sbjct: 117 GEPLALR 123
>XP_005273533
          Length = 1921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TPLALR 7
            TPLALR
Sbjct: 1421 TPLALR 1426
>XP_005263884
          Length = 554

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TPLALRAV 9
          TP A+RAV
Sbjct: 36 TPAAVRAV 43
>XP_006712870
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TPLALRAV 9
          TP A+RAV
Sbjct: 36 TPAAVRAV 43
>XP_005245015
          Length = 282

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  GTPLALR 7
          G PLALR
Sbjct: 25 GEPLALR 31
>XP_005245014
          Length = 375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GTPLALR 7
           G PLALR
Sbjct: 118 GEPLALR 124
>NP_065210
          Length = 1719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TPLALR 7
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_065209
          Length = 1881

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TPLALR 7
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_000028
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    TPLALR 7
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_005682
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>NP_064693
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>NP_001035830
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GTPLALRAV 9
          G P ALRA+
Sbjct: 48 GAPEALRAL 56
>NP_689498
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GTPLALRAV 9
          G PL  RA+
Sbjct: 33 GAPLPFRAI 41
>NP_115486
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  GTPLALRA 8
          G PL LRA
Sbjct: 53 GPPLLLRA 60
>XP_005253348
          Length = 1229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253347
          Length = 1502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_006719088
          Length = 1536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253346
          Length = 1536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253344
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253345
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253343
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253341
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PLALRAV 9
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005266178
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GTPLALRAV 9
          G PL  RA+
Sbjct: 33 GAPLPFRAI 41
>XP_005266176
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GTPLALRAV 9
          G PL  RA+
Sbjct: 33 GAPLPFRAI 41
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.139    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 617,483
Number of extensions: 861
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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